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Agro-industry vegetable residues valorisation : a waste biorefinery

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Presentation on theme: "Agro-industry vegetable residues valorisation : a waste biorefinery"— Presentation transcript:

1 Agro-industry vegetable residues valorisation : a waste biorefinery
Paola Di Donato Associate Researcher Institute of Biomolecular Chemistry Via Campi Flegrei 34, (80078) Napoli - ITALY November 20th, 2012 Città della Scienza, Naples, Italy

2 Institute of Biomolecular Chemistry Pozzuoli, Napoli
31 institutes  Padova Institute of Biomolecular Chemistry Pozzuoli, Napoli 45 institutes Rome  Naples  Sassari  31 institutes  Catania November 20th, 2012 Città della Scienza, Naples, Italy

3 November 20th, 2012 Città della Scienza, Naples, Italy
Born in 1967 as “Istituto per la Chimica e Fisica delle Molecole di Interesse Biologico” (ICMIB), “Institute for the Chemistry and Physics of Biologically Interesting Molecules”. In 2002 it moved to the new building in Pozzuoli (NA) and took the name of “Istituto di Chimica Biomolecolare (ICB-CNR)”. Institute of Biomolecular Chemistry that today includes also the Roma, Catania, Sassari and Padova branches. Main research activities in ICB are in the fields of organic chemistry, biochemistry and microbiology with main topics including environmental, pharmaceutical, food and biotechnological applications. November 20th, 2012 Città della Scienza, Naples, Italy

4 Agro-industry residues: a waste biomass
According the Directive 2001/77/EC of the European Community biomass is “the biodegradable fraction of products, waste and residues from agriculture (including vegetal and animal substances), forestry and related industries, as well as the biodegradable fraction of industrial and municipal waste” According the Directive 2001/77/EC of the European Community biomass is “the biodegradable fraction of products, waste and residues from agriculture (including vegetal and animal substances), forestry and related industries, as well as the biodegradable fraction of industrial and municipal waste” Source: November 20th, 2012 Città della Scienza, Naples, Italy

5 Residual biomass: yearly production in Italy
(source: C.R.P.A., Animal Production Research Centre, Italy)   November 20th, 2012 Città della Scienza, Naples, Italy

6 Agro-industry residual biomass in Italy: yearly amounts
Biomass available (thousand tons/year) Actual re-use strategy Wood transformation 4.400 Energy production Wood recycling 8.000 Paper Industry 270 Food Oil 450 Energy, composting Winery Canning and market packaging 340 Rice transformation 330 (source: Itabia )   November 20th, 2012 Città della Scienza, Naples, Italy

7 Agro-industry residual biomass: a resource that can be valorised
Environmental problem Economical issue …. but a significant fraction of biomolecules is lost after industrial processing, being discarded into the residual matter….. proteins, fibres, polysaccharides, sugars, polyphenols, carotenoids drugs nutraceuticals cosmetics biopolymers biofuels by-products that can represent the starting material for other processes raw materials not in competition with food chain November 20th, 2012 Città della Scienza, Naples, Italy

8 The biorefinery approach BIOMASS
Agro-industry residual biomass: a resource that can be valorised The biorefinery approach Food industry wastes (vegetables processing residues) Agriculture and crop residues BIOMASS Bioprocesses (bacteria, enzymes) Chemical Processes Physical Processes Transformation technologies Biofuels Antioxidants Biopolymers Energy and chemicals Adapted from Kamm, B.; Kamm, M. (2004). Principles of Biorefineries. Appl. Microbiol. Biotechnol. (AMB), 64: November 20th, 2012 Città della Scienza, Naples, Italy

9 dry waste gross composition
Agro-industry wastes biorefinery: ongoing research at the Institute of Biomolecular Chemistry dry waste gross composition Tomato Lemon Carrot Fennel Data from INRAN (Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione) November 20th, 2012 Città della Scienza, Naples, Italy

10 Agro-industry wastes biorefinery: ongoing research
at the Institute of Biomolecular Chemistry Polysaccharides recovery II generation bioethanol Antioxidants recovery November 20th, 2012 Città della Scienza, Naples, Italy

11 Isolation of tomato’s wastes polysaccharides
Tomato waste peels Chemical and physical characterization of isolated polysaccharides Alkali treatment at r.t. Polysaccharide MW (Da)> ; High viscosity; High thermal resistance; [a]D25-0,189.0 Glc/Xil/Gal/GalN/GlcN/Fuc (1:0.9:0.5:0.4:0.2:tr) Collaboration with Dr. Mario Malinconico and Dr. Barbara Immirzi (ICTP – CNR) Container and object construction Innovative material for containers or for green house applications November 20th, 2012 Città della Scienza, Naples, Italy

12 Isolation of tomato’s wastes polysaccharides
biotechnological applications Biodegradable film for solarization or mulching applications Collaboration with Dr. Mario Malinconico and Dr. Barbara Immirzi (ICTP – CNR) Tomato waste peels Alkali treatment at r.t. Polysaccharide Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, et cetera biological properties Inhibition of NF-kB/DNA binding activity November 20th, 2012 Città della Scienza, Naples, Italy

13 vegetable wastes polysaccharides extraction: ongoing research
fennel residues lemon wastes (pomace) carrot residues Maceration Ultrasound assisted Microwaves assisted alkali/acid extraction Structural analysis (MW; monomer composition; spectroscopic analysis) Thermogravimetrical analysis POLYSACCHARIDES Biotechnological applications Biological properties November 20th, 2012 Città della Scienza, Naples, Italy

14 Agro-industry wastes biorefinery: ongoing research
at the Institute of Biomolecular Chemistry Polysaccharides recovery II generation bioethanol Antioxidants recovery November 20th, 2012 Città della Scienza, Naples, Italy

15 vegetable wastes polyphenols extraction: ongoing research
tomato residues fennel residues lemon wastes (pomace) carrot residues Maceration Ultrasound assisted Microwaves assisted MeOH; EtOH; MeOH/H2O; EtOH/H2O Structural analysis Antioxidant and radical scavenging activities Biological activity assay Biotechnological applications Biological properties POLYPHENOLS November 20th, 2012 Città della Scienza, Naples, Italy

16 Vegetable wastes polyphenols extraction
work in progress….. Project PON01_01966 Integrated agro-industrial chains with high energy efficiency for the development of eco-compatible processes of energy and biochemicals production from renewable sources and for the land valorisation (EnerbioChem) Naples University “Federico II” November 20th, 2012 Città della Scienza, Naples, Italy

17 Agro-industry wastes biorefinery: ongoing research
at the Institute of Biomolecular Chemistry Polysaccharides recovery II generation bioethanol Antioxidants recovery November 20th, 2012 Città della Scienza, Naples, Italy

18 Agro-industry wastes: production of enzymes for second generation bioethanol from extremophiles
Bilateral Project Italy-Turkey “Extremophiles for Next-Generation Biofuels ” Dr. Barbara Nicolaus Prof. Ebru Toksoy Oner Vegetable wastes under investigation: potato peels, carrot, tomato, sugar beet pulp, starch and sugar beet molasses November 20th, 2012 Città della Scienza, Naples, Italy

19 Agro-industry wastes: production of second generation bioethanol
I generation bioethanol II generation bioethanol Waste Biomass* glucose * Not in competition with food chain CH3CH2OH Starch (energy crops) liquefaction by thermostable amylases (starch cooking) Starch degradation and sugar Fermentation by engineered yeasts: expression of raw starch degrading enzymes in Saccharomyces spp Simultaneous Saccharification and Fermentation T = °C dextrins saccharification by thermostable amylases T = 65°C glucose T = 45°C Yeasts Fermentation (Saccharomyces spp) CH3CH2OH November 20th, 2012 Città della Scienza, Naples, Italy

20 Amylolytic thermophile
Agro-industry wastes: production of enzymes for second generation bioethanol from extremophiles Amylolytic thermophile synthetic media Agro-industry wastes as culture media for extremophiles’ fermentation Geobacillus thermoleovorans (strain PIZZOT) lemon tomato raw starch degrading amylase (starch digestion to glucose without cooking pre-treatment) wastes as culture media for amylase production Complex medium Lemon medium 16.25 U/mg protein 6.375 U/mg protein Di Donato P., G. Fiorentino G., Anzelmo G., Tommonaro G., Nicolaus B., Poli A “Waste Biomass Valorization” 2: Optical microscopy observation of raw starch before (a) and after (b) digestion by G. thermoleovorans amylase November 20th, 2012 Città della Scienza, Naples, Italy

21 November 20th, 2012 Città della Scienza, Naples, Italy
In conclusion….. by means of an integrated biorefinery approach waste biomass from agro-industry can be valorised to give Fibers and polysaccharides for biodegradable polymers production Biomolecules for several applications: antioxidants and bioactive molecules for pharmaceutical, cosmetic or food applications Microorganisms and enzymes for second generation biofuels production November 20th, 2012 Città della Scienza, Naples, Italy

22 November 20th, 2012 Città della Scienza, Naples, Italy
Thanks to…. Dott. Barbara Nicolaus Dott. Annarita Poli Dott. Licia Lama Dott. Giuseppina Tommonaro Prof. Ebru Toksoy Oner “If you want to go fast, go alone.  If you want to go far, go together” African proverb November 20th, 2012 Città della Scienza, Naples, Italy

23 Thanks for your attention!
Dott. Paola Di Donato Grönlund-Nisunen - Wave of Matter 2012


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