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Paola Di Donato Paola Di Donato Associate Researcher Agro-industry vegetable residues valorisation : a waste biorefinery Institute.

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Presentation on theme: "Paola Di Donato Paola Di Donato Associate Researcher Agro-industry vegetable residues valorisation : a waste biorefinery Institute."— Presentation transcript:

1 Paola Di Donato Paola Di Donato Associate Researcher Agro-industry vegetable residues valorisation : a waste biorefinery Institute of Biomolecular Chemistry Via Campi Flegrei 34, (80078) Napoli - ITALY November 20th, 2012 Città della Scienza, Naples, Italy

2 31 institutes 45 institutes Naples Rome Sassari Catania Padova Institute of Biomolecular Chemistry Pozzuoli, Napoli November 20th, 2012 Città della Scienza, Naples, Italy

3 Born in 1967 as Istituto per la Chimica e Fisica delle Molecole di Interesse Biologico (ICMIB), Institute for the Chemistry and Physics of Biologically Interesting Molecules. In 2002 it moved to the new building in Pozzuoli (NA) and took the name of Istituto di Chimica Biomolecolare (ICB-CNR). Institute of Biomolecular Chemistry that today includes also the Roma, Catania, Sassari and Padova branches. Main research activities in ICB are in the fields of organic chemistry, biochemistry and microbiology with main topics including environmental, pharmaceutical, food and biotechnological applications. November 20th, 2012 Città della Scienza, Naples, Italy

4 According the Directive 2001/77/EC of the European Community biomass is the biodegradable fraction of products, waste and residues from agriculture (including vegetal and animal substances), forestry and related industries, as well as the biodegradable fraction of industrial and municipal waste Agro-industry residues: a waste biomass Source: November 20th, 2012 Città della Scienza, Naples, Italy

5 (source: C.R.P.A., Animal Production Research Centre, Italy) Residual biomass: yearly production in Italy November 20th, 2012 Città della Scienza, Naples, Italy

6 IndustryBiomass available (thousand tons/year) Actual re-use strategy Wood transformation 4.400Energy production Wood recycling 8.000Energy production Paper Industry 270Energy production Food Oil 450Energy, composting Winery 450 Energy, composting Canning and market packaging 340 Energy, composting Rice transformation 330 Energy, composting (source: Itabia ) Agro-industry residual biomass in Italy: yearly amounts November 20th, 2012 Città della Scienza, Naples, Italy

7 Environmental problem Economical issue by-products that can represent the starting material for other processes raw materials not in competition with food chain proteins, fibres, polysaccharides, sugars, polyphenols, carotenoids drugsnutraceuticalscosmeticsbiopolymersbiofuels Agro-industry residual biomass: a resource that can be valorised …. but a significant fraction of biomolecules is lost after industrial processing, being discarded into the residual matter….. November 20th, 2012 Città della Scienza, Naples, Italy

8 The biorefinery approach Adapted from Kamm, B.; Kamm, M. (2004). Principles of Biorefineries. Appl. Microbiol. Biotechnol. (AMB), 64: Food industry wastes (vegetables processing residues) Agriculture and crop residues Bioprocesses (bacteria, enzymes) Chemical Processes Physical Processes Biofuels Antioxidants Biopolymers Agro-industry residual biomass: a resource that can be valorised November 20th, 2012 Città della Scienza, Naples, Italy

9 Agro-industry wastes biorefinery: ongoing research at the Institute of Biomolecular Chemistry Data from INRAN (Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione) dry waste gross composition TomatoLemon Carrot Fennel November 20th, 2012 Città della Scienza, Naples, Italy

10 Polysaccharides recovery II generation bioethanol Antioxidants recovery Agro-industry wastes biorefinery: ongoing research at the Institute of Biomolecular Chemistry November 20th, 2012 Città della Scienza, Naples, Italy

11 Isolation of tomatos wastes polysaccharides Tomato waste peels November 20th, 2012 Città della Scienza, Naples, Italy Alkali treatment at r.t. Polysaccharide MW (Da)> ; High viscosity; High thermal resistance; [ ]D25-0,189.0 Glc/Xil/Gal/GalN/GlcN/Fuc (1:0.9:0.5:0.4:0.2:tr) Chemical and physical characterization of isolated polysaccharides Container and object construction Innovative material for containers or for green house applications Collaboration with Dr. Mario Malinconico and Dr. Barbara Immirzi (ICTP – CNR)

12 November 20th, 2012 Città della Scienza, Naples, Italy Isolation of tomatos wastes polysaccharides Tomato waste peels Alkali treatment at r.t. Polysaccharide biotechnological applications Biodegradable film for solarization or mulching applications Collaboration with Dr. Mario Malinconico and Dr. Barbara Immirzi (ICTP – CNR) Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, et cetera biological properties Inhibition of NF- k B/DNA binding activity

13 alkali/acid extraction POLYSACCHARIDES vegetable wastes polysaccharides extraction: ongoing research fennel residues Structural analysis (MW; monomer composition; spectroscopic analysis) Thermogravimetrical analysis November 20th, 2012 Città della Scienza, Naples, Italy lemon wastes (pomace) carrot residues Biotechnological applications Biological properties Maceration Ultrasound assisted Microwaves assisted

14 Polysaccharides recovery II generation bioethanol Antioxidants recovery Agro-industry wastes biorefinery: ongoing research at the Institute of Biomolecular Chemistry November 20th, 2012 Città della Scienza, Naples, Italy

15 MeOH; EtOH; MeOH/H2O; EtOH/H2O POLYPHENOLS vegetable wastes polyphenols extraction: ongoing research Structural analysis Antioxidant and radical scavenging activities Biological activity assay November 20th, 2012 Città della Scienza, Naples, Italy Biotechnological applications Biological properties Maceration Ultrasound assisted Microwaves assisted tomato residues fennel residues lemon wastes (pomace) carrot residues

16 Vegetable wastes polyphenols extraction Project PON01_01966 Integrated agro-industrial chains with high energy efficiency for the development of eco-compatible processes of energy and biochemicals production from renewable sources and for the land valorisation (EnerbioChem) work in progress….. Naples University Federico II November 20th, 2012 Città della Scienza, Naples, Italy

17 Agro-industry wastes biorefinery: ongoing research at the Institute of Biomolecular Chemistry Polysaccharides recovery II generation bioethanol Antioxidants recovery November 20th, 2012 Città della Scienza, Naples, Italy

18 Agro-industry wastes: production of enzymes for second generation bioethanol from extremophiles Vegetable wastes under investigation: potato peels, carrot, tomato, Vegetable wastes under investigation: potato peels, carrot, tomato, sugar beet pulp, starch and sugar beet molasses Bilateral Project Italy-Turkey Extremophiles Extremophiles for Next-Generation Biofuels Dr. Barbara Nicolaus Prof. Ebru Toksoy Oner November 20th, 2012 Città della Scienza, Naples, Italy

19 Yeasts Fermentation (Saccharomyces spp) I generation bioethanol dextrins CH 3 CH 2 OH Agro-industry wastes: production of second generation bioethanol Starch degradation and sugar Fermentation by engineered yeasts: expression of raw starch degrading enzymes in Saccharomyces spp Simultaneous Saccharification and Fermentation II generation bioethanol Waste Biomass* glucose * Not in competition with food chain CH 3 CH 2 OH Starch (energy crops) November 20th, 2012 Città della Scienza, Naples, Italy liquefaction by thermostable amylases (starch cooking) T = °C glucose T = 45°C saccharification by thermostable amylases T = 65°C

20 synthetic media Agro-industry wastes as culture media for extremophiles fermentation Di Donato P., G. Fiorentino G., Anzelmo G., Tommonaro G., Nicolaus B., Poli A Waste Biomass Valorization 2: tomato lemon Complex mediumLemon medium U/mg protein6.375 U/mg protein Agro-industry wastes: production of enzymes for second generation bioethanol from extremophiles Geobacillus thermoleovorans (strain PIZZO T ) Amylolytic thermophile raw starch degrading amylase (starch digestion to glucose without cooking pre-treatment) Optical microscopy observation of raw starch before (a) and after (b) digestion by G. thermoleovorans amylase November 20th, 2012 Città della Scienza, Naples, Italy wastes as culture media for amylase production

21 Fibers and polysaccharides for biodegradable polymers production Biomolecules for several applications: antioxidants and bioactive molecules for pharmaceutical, cosmetic or food applications Microorganisms and enzymes for second generation biofuels production by means of an integrated biorefinery approach waste biomass from agro-industry can be valorised to give In conclusion….. November 20th, 2012 Città della Scienza, Naples, Italy

22 Dott. Barbara Nicolaus Dott. Annarita Poli Dott. Licia Lama Dott. Giuseppina Tommonaro If you want to go fast, go alone. If you want to go far, go together African proverb Thanks to…. November 20th, 2012 Città della Scienza, Naples, Italy Prof. Ebru Toksoy Oner

23 Thanks for your attention! Dott. Paola Di Donato Grönlund-Nisunen - Wave of Matter 2012


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