Presentation is loading. Please wait.

Presentation is loading. Please wait.

Journal of Allergy and Clinical Immunology

Published byPiers Perry Modified over 4 years ago

Similar presentations

Presentation on theme: "Journal of Allergy and Clinical Immunology"— Presentation transcript:

1 Journal of Allergy and Clinical Immunology
Development of a novel Ara h 2 hypoallergen with no IgE binding or anaphylactogenic activity  Angelika Tscheppe, MSc, Dieter Palmberger, PhD, Leonie van Rijt, PhD, Tanja Kalic, MSc, Vanessa Mayr, MSc, Chiara Palladino, MSc, Claudia Kitzmüller, PhD, Wolfgang Hemmer, PhD, Christine Hafner, MD, Merima Bublin, PhD, Ronald van Ree, PhD, Reingard Grabherr, PhD, Christian Radauer, PhD, Heimo Breiteneder, PhD  Journal of Allergy and Clinical Immunology  DOI: /j.jaci Copyright © 2019 The Authors Terms and Conditions

2 Journal of Allergy and Clinical Immunology DOI: (10.1016/j.jaci.2019.08.036)
Copyright © 2019 The Authors Terms and Conditions

3 Fig 1 Construction of Ara h 2 derivatives. A, Structural representations of native and red/alk proteins. B, Amino acid sequences of wtAra h 2 and mtAra h 2 showing the IgE-binding epitopes removed in mtAra h 2. Previously determined linear IgE epitopes are boxed, immunodominant T-cell epitopes are shown in boldface, and helices are shown in gray. Journal of Allergy and Clinical Immunology DOI: ( /j.jaci ) Copyright © 2019 The Authors Terms and Conditions

4 Fig 2 IgE binding to Ara h 2 derivatives tested by means of ELISA with 48 Ara h 2–sensitized patients with peanut allergy. A, Distributions of ELISA OD values in all patients. *P < .05 and ***P < .001. B, IgE ELISA and inhibition ELISA results of 5 representative patients. For the inhibition ELISA, nAra h 2 was immobilized, and residual IgE binding after preincubating sera with Ara h 2 derivatives was measured. C, Comparison of IgE binding to native and red/alk proteins (lacking conformational epitopes) in individual patients. D, Individual epitope recognition patterns demonstrated by comparing IgE binding to wtAra h 2, mtAra h 2 (lacking linear epitopes), and red/alk wtAra h 2 (lacking conformational epitopes). Journal of Allergy and Clinical Immunology DOI: ( /j.jaci ) Copyright © 2019 The Authors Terms and Conditions

5 Fig 3 Correlation of Ara h 2–specific IgE levels with measures of epitope recognition patterns derived from IgE ELISA data. A, The mtAra h 2 to wtAra h 2 ratio represents IgE binding to conformational epitopes. B, The red/alk to native wtAra h 2 ratio represents IgE binding to linear epitopes. Journal of Allergy and Clinical Immunology DOI: ( /j.jaci ) Copyright © 2019 The Authors Terms and Conditions

6 Fig 4 Basophil activation tests (n = 7) with native and red/alk proteins. Areas under the concentration-dependent activation curves are shown. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology DOI: ( /j.jaci ) Copyright © 2019 The Authors Terms and Conditions

7 Fig 5 T cell–stimulating abilities of Ara h 2 derivatives. A, In vitro proliferation assays with PBMCs from Ara h 2–sensitized patients with peanut allergy (n = 9). Data are presented as relative SIs normalized to the SIs of cells treated with nAra h 2. B, Flow cytometry–based proliferation assay with PBMCs of a representative patient. Data represent percentages of proliferating (carboxyfluorescein N-succinimidyl ester–low) cells among CD4+ T cells (CD3+CD8− cells). Journal of Allergy and Clinical Immunology DOI: ( /j.jaci ) Copyright © 2019 The Authors Terms and Conditions

8 Fig 6 Anaphylactic response in C3H/HeOuJ mice with peanut allergy after challenge with native and red/alk proteins. A, Mice were sensitized intragastrically (i. g.) with PE and challenged intraperitoneally (i. p.) on day 37 with native and red/alk proteins. B, Ara h 2–specific IgE and IgG1 levels of peanut-sensitized mice and control mice sensitized by PBS. **P < .01. C, Maximum core body temperature decrease after intraperitoneal challenge measured over the period of 1 hour every 10 minutes. *P < .05. Journal of Allergy and Clinical Immunology DOI: ( /j.jaci ) Copyright © 2019 The Authors Terms and Conditions

Download ppt "Journal of Allergy and Clinical Immunology"

Similar presentations

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,
Luise Westernberg, PhD, Véronique Schulten, PhD, Jason A

Luise Westernberg, PhD, Véronique Schulten, PhD, Jason A
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3  Merima Bublin, PhD, Maria Kostadinova,

IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3  Merima Bublin, PhD, Maria Kostadinova,
Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.
Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.
Molecular mimicry between cockroach and helminth glutathione S-transferases promotes cross-reactivity and cross-sensitization  Helton C. Santiago, MD,

Molecular mimicry between cockroach and helminth glutathione S-transferases promotes cross-reactivity and cross-sensitization  Helton C. Santiago, MD,
Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,

Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,
Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,

Mapping of conformational IgE epitopes on Phl p 5a by using mimotopes from a phage display library  Brigitte Hantusch, MSc, Sigurd Krieger, TA, Eva Untersmayr,
Enhanced Pru p 3 IgE-binding activity by selective free fatty acid-interaction  Pawel Dubiela, MSc, Roberta Aina, PhD, Dominika Polak, MSc, Sabine Geiselhart,

Enhanced Pru p 3 IgE-binding activity by selective free fatty acid-interaction  Pawel Dubiela, MSc, Roberta Aina, PhD, Dominika Polak, MSc, Sabine Geiselhart,
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.
Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,
Protein corona–mediated targeting of nanocarriers to B cells allows redirection of allergic immune responses  Limei Shen, PhD, Stefan Tenzer, PhD, Wiebke.

Protein corona–mediated targeting of nanocarriers to B cells allows redirection of allergic immune responses  Limei Shen, PhD, Stefan Tenzer, PhD, Wiebke.
Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,
Rush immunotherapy results in allergen-specific alterations in lymphocyte function and interferon-γ production in CD4+ T cells  Gideon Lack, MD, Harold.

Rush immunotherapy results in allergen-specific alterations in lymphocyte function and interferon-γ production in CD4+ T cells  Gideon Lack, MD, Harold.

Similar presentations

Ads by Google