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Improvement of insect ecdysome on the hatching rate of Artemia eggs Jia Qinxian 1 Chen Lijing 2 Zhen Mianping 1 1 Open Laboratory of Saline Lake Resources.

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Presentation on theme: "Improvement of insect ecdysome on the hatching rate of Artemia eggs Jia Qinxian 1 Chen Lijing 2 Zhen Mianping 1 1 Open Laboratory of Saline Lake Resources."— Presentation transcript:

1 Improvement of insect ecdysome on the hatching rate of Artemia eggs Jia Qinxian 1 Chen Lijing 2 Zhen Mianping 1 1 Open Laboratory of Saline Lake Resources and Environment, The Chinese Academy of Geological Sciences, Beijing, China 2 Shanghai Fisheries University, Shanghai, China

2 Content u 1. Introduction u 2.Treatment and Establish normal criterion method u 3. Results u 4. Conclusion

3 1.Introduction C oncept Diapause and Dormancy Diapause----- Dormancy----

4 1. Introduction: Understanding of Artemia eggs structure Outer membrane Outer lay shuck Inner lay shuck Embryon Embryonic membrane Inner membrane

5 1. Introduction: Understanding of Artemia eggs structure Embryon Embryonic membrane

6 1. Introduction :Study progress of Artemia eggs diapause (1) Structure Outer membrane Diapause Activation Morris and Afzelius (1967): Outer membrane broken Anderson et al. (1970) : shuck thinned

7 1.Introduction :Study progress of Artemia eggs diapause (2) Structure Diapause Lavens and Sorgeloos (1987) Diapause New productive eggs Decapsulation but no activation

8 1.Introduction :Study progress of Artemia eggs diapause (3) pHi pH i pHi>7.9 diapause eggs Diapause Activation pHi Some scholars find that the eggs was diapause at pHi over7.9 and guess the diapause eggs will activation at pHi reduced to acidity

9 1. Introduction :Study progress of Artemia eggs diapause (4) Biotic active matter Biotic active matter Dutrieu (1960) Nelis et al. (1984 a,b; 1987) Konrad Dabrowski (19991) Biotic active mater have important regulation for diapause eggs activition

10 1. Introduction :Study progress of Artemia eggs diapause (5) problem The Artemia eggs diapause mechanism not be complete comprehended The methods of activation of diapause eggs was not always efficiency for all strains Up to now, there are a great lot of low hatching quality eggs produced in Asia,Europe and other places. Which should be improvement.

11 1. Introduction : Some study progress of diapause in insects and other animal 1976 insects hormone The diapause eggs become active eggs when treatment by insect hormone

12 2. Materials Artemia strains

13 u Chemical method: u 3% H 2 O 2 5min 10% NaClO 5min u 5% NH 4 OH 5min 5% MgCl 2 60min u 5% CH 3 OH 30min 1% NaOH 5min u 1M HCl 60min 5%CH2O 5min u Physical method : u -25 freeze 4 to 20 weeks u re-dehydrate 3 times u Hormone: Insect ecdysome [ 2, 3, 14, 22(R), 25- u Pentahydroxy -7- cholesten-6-one]

14 2.Methods (1) Hormone treatment u Concentration u 0~90ppb 5~25ppm u Dry Dipping Hormone Hatching u eggs 180 min treatment u 30~150 min u time Fixing 24 h and 48h hatching rate Fixing

15 u Dehydration Paraffine embedFixing Slice up DyeingObservation under microscope Dry eggs Observation

16 2. Methods (2) Observation : dye cells/not dye cells by Gimmsa biotic pigment cell division (dye cell) cell no division (not dye cell) Aim : Establish normal criterion for more times examining and could be calculated easily

17 3.Results -- (1) Establish normal criterion Comparison of dye/not dye cells of dry eggs* *Collected and experimental time of Artemia eggs: 1995~1996

18 (1) Establish normal criterion Comparison of dye/not dye cells of wet eggs* *Collected and experimental time of Artemia eggs: 1995~1996

19 (1) Establish normal criterion Hatching rate (%) and Dye cells/not Dye cells Strains Dye cells / not dye cells Hatching rate (%) (Collected and experimental time of Artemia eggs: 1995~1996)

20 (1) Establish normal criterion Quality grading criterion Hatching rate The rate of dye cells / not dye cells

21 3. Results (2) Hormone treatment SHY strain (from Haiyang of Shangdong, China ) min Treatment time The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 1999~2000)

22 (2) Hormone treatment SHY strain( Haiyang of Shangdong, China ) Treatment dosage ppb The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 1999~2000)

23 (2) Hormone treatment RUS strain( Russia) Treatment time min The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 2000~2001)

24 (2) Hormone treatment RUS strain ( Russia ) ppb Treatment dosage The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 2000~2001)

25 (2) Hormone treatment QGH strain(fromGahai of Qinghai,China ) Treatment time min The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 1999~2001)

26 (2) Hormone treatment QGH strain(fromGahai of Qinghai,China ) Treatment dosage ppb The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 1999~2001)

27 (2) Hormone treatment KSK strain(from Kazakhstan) Treatment time min The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 2000~2001)

28 (2) Hormone treatment KSK strain (from Kazakhstan) Treatment dosage 45 ppb The rate of dye cells / not dye cells (Collected and experimental time of Artemia eggs: 2000~2001)

29 Efficiency compare of different treatment method Hatching rate

30 4.Conclusion u The superexcellent concentration and fitness treatment time of each strain is difference. u The raise of the hatching quality is very obvious at its fit concentration and time. u The shortage concentration of hormone will no obvious effect on most strains u

31 4.Conclusion u More over concentration of hormone will obvious decrease the hatching rate. When the concentration over 35 ppb the hatching rate was maybe littler than that of no treatment u At the ecdysome concentration range 10 to 20 ppb and the treatment time range 30 to 60 min, the hatching rate of most strains was greatly improved.

32 4.Conclusion u At superexcellent concentration and fitness treatment time, the hormone treatment was higher than the treatment by chemical reagent and physical method (-25 freeze 4 to 20 weeks). u The highest hatching of Haiyang strain was over 57.1% than no treatment, over 10.5% than treatment by H 2 O 2 and freeze.

33 Thank you very much END


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