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Bio 115 Ecology and Evolution MENDELIAN GENETICS.

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1 Bio 115 Ecology and Evolution MENDELIAN GENETICS

2 Bio 161Lesson 01 Ovists and Spermists are both Preformationists. Although people realized that traits were passed on from parents to children by way of reproduction, the underlying mechanisms remained largely unknown. The preformationists believed that all life was preformed at the moment of creation and that successive generations of individuals were encased, one inside the other, in increasingly small versions of their adult selves. Thus, children started as miniaturized adults whose development was triggered by the act of mating. The ovists (Paracelsus, Jan Swammerdam, and Reinier de Graaf, accepted that the offspring was contained in a preformed state in the egg cell and that its development was triggered by the juices of the sperm cell. In contrast, the spermists claimed that the offspring sprung from the sperm cell and that the female only provided a nurturing womb for the new life to develop. Spermism had to await the discovery of sperm cells by the first microscopists (Anthony van Leeuwenhoek and Nicolas Hartsoeker) who discovered the homunculus or little man hidden within the sperm cell. Jan SwammerdamReinier de Graaf Anthony van Leeuwenhoiek Nicolas Hartsoeker Mendelian heredity: Ovists and Spermists. Her103

3 Bio 161Lesson 01 Pangenesis. Pangenesis states that traits are inherited directly. Elements or information of each of the parents body parts are transmitted to the offspring directly, and independently of the information of other parts. The child is formed after this hereditary material from all parts of the parents bodies has come together. Hippocrates called this reproductive material gonos or seed. Darwin (1868) suggested that all cells and tissues excrete this material as microscopic granules or gemmules. The pangenesis theory assumes that both male and female contribute to the offspring and that the traits blend in the offspring. Conclusion: The two classical assumptions lead to a paradox. Indeed, if variation and heredity occur within the boundaries of the species (no variation enters from outside the species) and the traits of the parents are blended in the offspring, than sooner or later all members of the same species should have the same appearance. Which is not the case. In addition, early experimental work of Josef Kolreuter (1760) and TA Knight (1790) casted doubt on the classical assumptions. Mendelian heredity: Pangenesis and Blending Her104 Distinction between soma and germ line. In pangenesis, information from the somatic cells (gemmules) is collected in the germ cells and passed on to the offspring.

4 Bio 161Lesson 01 Observations cast doubt on the classic assumptions: Josef Kolreuter (1760) crossed different strains of tobacco. The resulting hybrids differed in appearance from both parent strains. When individuals of the hybrid generation were crossed, their offspring were highly variable: some resembling the hybrid generation (their parents) others resembling the original strains (their grandparents). T.A. Knight (1790) crossed a strain of peas with purple flowers with one with white flowers. All hybrid offspring had purple flowers. Among the offspring of these hybrids, however, were some plants with purple and some with white flowers. Because traits could be masked in one generation to reappear in the next, inheritance could not occur by direct transmission of traits (How could a trait that is transmitted directly disappear and reappear?). Similarly, blending theory can not explain differences between offspring of the same parents. Mendelian heredity: classic assumptions fail Her105 Josef kolreuter

5 Bio 161Lesson 01 Mendelian heredity: lamarckism. Her106 Lamarckism: inheritance of acquired traits: Aristole ( BC) proposed that the physical, intellectual, and personality characteristics acquired by people during their life time were passed on to their offspring. According to this theory, musical talent, cycling performance, or soccer aptitute can be passed on from the parents to their children if the parents dedicate their life to the pursuit of these activities because their genetic material is affected by their fervent dedication. Jean-Baptiste Lamarck ( ) used the idea to explain the mechanism of heredity and suggested that by simply using or not using certain organs, organs and body parts may be highly developed or atrophioed and the offspring can then inherit these acquired traits. Lamarckism rejected

6 Bio 161Lesson 01 Lamarckism rejected; Lamarckism, or the theory of inheritance of acquired traits, influenced scientific thinking for over 200 years. However, there are obvious problems to this idea. Children with normal limbs are begot from deformed parents. Also, think of the jewish practice of circumcisuin. August Weismann ( ) nipped the idea of inheriting acquired characteristics in the bud so to speak when he cut the tails off of rats and mated them, showing that the offspring still had tails. Mendelian heredity: lamarckism rejected Her106a

7 Bio 161Lesson 02 Who is Mendel? Gregor Johann Mendel (born on 22 July 1822 in Heizendorf, Austria [Hyncice, Czech Republic] and died on January 6, 1884 in Brno [Brunn], Austria) was the only son of a farmer and attended local schools and the Philosophic Institute at Olomouc. In 1843, he entered the Augustinian Order at st. Thomas Monastery in Brunn and began his theological studies. He was ordained in From 1851 to 1853 he studied zoology, botany, chemistry, and physics at the University of Vienna. Two professors were especially influentiual: the physicist Christian Doppler (who instilled a sense for experimental work and mathematical modeling) and the botanist Franz Unger (who aroused interest in the causes of variation in plants). Mendel returned to the monastery in 1854 to teach part-time at a school where other teachers shared his enthusiasm for sciences. Moreover, St. Thomas Monastery was a center of creative interest in the sciences and culture. There were well-known philosophers, a musicologist, mathematicians, mineralogists, and botanists who were heavily engaged in scientific research and teaching. Most important, the monks had a long- standing interest in the breeding of plants. Mendel completed his experimental work with the garden peas between 1854 and Mendel tried to determine whether it was possible to obtain new variants of peas by cross-breeding. He presented his work in two lectures before the Society for the Study of the Natural Sciences in Brunn in His paper, Versuche uber Pflanzen-Hybriden (Experiments in plant hybridization) was published in the Societys Proceedings in His work, however, was largely ignored. In the spring of 1900, three botanists (Hugo de Vries, Karl Correns, and E. von Tschermak) reported independent verifications of Mendels work. Her201

8 Bio 161Lesson 02 Why Mendel chose the garden pea: Earlier investigators had produced hybrids by crossing different strains. A large number of true-breeding varieties were available. Pea plants are small, easy to grow, and have a relative short generation time. It is easy to control self fertilization and cross-fertilization. Self-fertilization: Both male and female sexual organs are enclosed in the same flower and the male and female gametes produced can fuse to form viable offspring. Self- fertilization takes place automatically within an individual flower if it is not disturbed. Cross-fertilization: Cross-fertilization is the result of cross-pollination. The latter occurs when pollen of a different flower is introduced to the female stigma of a flower after first removal of the petals and male anthers of the flower. Why Mendel chose the garden pea. Her202

9 Bio 161Lesson 02 Mendels experimental design: Mendel selected pea varieties that differed in 7 traits from each other. He conducted his crosses in three stages:pea varieties that differed in 7 traits (1)He allowed pea plants of a given variety to produce progeny by self-fertilization for several generations. Mendel confirmed that the traits of the variants he studied were true (pure)-breeding (or homozygous). All white flowered plants produced white-flowered offspring regardless of the number of generations. He called these plants the Parental generation or P generation. (2) Mendel than performed crosses between varieties showing alternative forms of the trait in which he was interested. E.g., cross a white flowered female with a purple flowered male. (He also carried out the reciprocal cross!) He called the offspring of the P generation the first Filial or F1 generation.(He also carried out the reciprocal cross!) (3) Finally, Mendel permitted the F1 hybrids to self-fertilize for several generations. He carefully counted and recorded the numbers of offspring exhibiting each trait in each succeeding generation. Mendels experimental design. Her203

10 Bio 161Lesson 02 What Mendel found: In a cross of white-flowered plants with purple- flowered plants, the F1 offspring all had purple flowers. Of the F2 offspring 25% had the recessive white flowers and 75% were purple-flowered. All recessive, white-flowered offspring were true- breeding (homozygous). In contrast only 1/3 of the dominant, purple-flowered offspring were true- breeding, while 2/3 were not. What Mendel found: purple- x white- flowers. In general: Her204

11 Bio 161Lesson 02 What Mendel found: In a cross between two contrasting varieties of peas the F1 offspring did not show properties intermediate of those of the parents, as the blending theory predicted. In every case, the offspring resembled one of the parents. The form of the trait expressed in the F1 is said to be dominant; the alternative form of the trait is said to be recessive. Some human traits. ¾ (75%) of the F2 exhibited the dominant trait, and ¼ (25%) of the F2 offspring displayed the recessive trait. The dominant: recessive ratio among the F2 plants was always 3:1. The 3:1 ratio of the F2 generation was really a disguised 1:2:1 ratio with ¼ true-breeding dominant, 2/4 non-true-breeding dominant, and ¼ true-breeding recessive individuals. What Mendel found: in general Her204a Results of Mendels other experiments.

12 Bio 161Lesson 02 Results of Mendels other experiments. Her204a1

13 Bio 161Lesson 02 To explain his results/ observations, Mendel proposed a simple model for inheritance of traits. Parents do not transmit physiological traits directly to their offspring. Rather, they transmit discrete packages of information (Mendels factors, or genes) that encode the trait. Mendel expressed the factors by a set of symbols. The dominant purple color is represented by P (upper case) and the recessive white color by p (lower case). Each individual receives two factors of the same trait, one from the father and one from the mother. Individuals are said to be diploid. There are alternative forms or alleles of a factor. An individual with thwo identical alleles of a trait is homozygous; an individual with two different alleles of a trait is heterozygous. The genotype of an individual that is true-breeding white (pp) and purple (PP) are homozygous. Heterozygous individuals have the genotype Pp. (continued(continued) Mendels Model of Heredity (part 1) Her205

14 Bio 161Lesson 02 To explain his results/ observations, Mendel proposed a simple model for inheritance of traits. (continued) The two alleles, one contributed by the male gamete and one by the female gamete, remain discrete units in the cells that develop into the new individual. Thus, when the individual matures and produces its own gametes, the alleles for each trait segregate randomly into these gametes. In heterozygous individuals, only one allele (the dominant) is expressed, while the other (the recessive) allele is present but not expressed. The genotype is the totality of the alleles of an individual and the phenotype is the actual physical appearance of the individual. The presence of a particular allele does not ensure that the alternative form of the trait encoded by it will be expressed in an individual carrying that allele. Mendels Model of Heredity (part 1) Apply to the Purple x White cross. Apply to the Yellow x Green cross. Her205a Examples of human traits.

15 Bio 161Lesson 02 Mendels cross of Purple x white flowered peas. Her205a01 Note. The cross between two individuals each heterozygous for a single trait is called a monohybrid cross.

16 Bio 161Lesson 02 Mendels cross of Green x yellow peas. A cross of true-breeding green and yellow pea pods yields all green offspring. The F2 generation yields 75% green and 25% yellow pea-pods. Her205a02

17 Bio 161Lesson 02 Human dominant and recessive traits. Many traits in humans also exhibit simple dominance or recessive inheritance, similar to the traits Mendel studied in peas. Her205a03

18 Bio 161Lesson 02 A.Parental cross (P) and F 1 generation: Cross of true-breeding purple-flowered female and true-breeding white-flowered male. P PP (female genotype) (female gametes) p p pp (male genotype) (male gametes) Place the different possible types of female gametes along the top of the square (because the female is homozygous all gametes are P, purple) and place all possible different types of male gametes (all are white, p) along the side of the square. PP p p PpPp PpPp PpPp PpPp Each potential zygote can be represented by combining a female (column) and male (row) gamete. Mendels model, analyzed by way of a Punnett square, clearly predicts that all F 1 offspring will be heterozugous and purple. Mendels interpretation of the crosses: the Punnett square (P cross). Her206

19 Bio 161Lesson 02 B. The F 1 cross and F 2 generation: Cross (or self-fertilization) of heterozygous, purple, male and female F 1 plants. PpPp pP (female genotype) (female gametes) P p PpPp (male genotype) (male gametes) Place the different possible types of female gametes (P and p) along the top of the square and place all possible different types of male gametes (P and p) along the side of the square. Pp P p P PpPp PpPp pp Each potential zygote can be represented by combining a female (column) and male (row) gamete. Mendels interpretation of the crosses: the Punnett square (F 1 cross) Her206a

20 Bio 161Lesson 02 1 st Law of Heredity or Law of Segregation. Mendels 1st l\Law of Heredity or the Law of Segregation. Mendels model thus accounts perfectly for the phenotypic ratio of offspring observed in each cross, and thereby, explains how traits are inherited, that is, the process of heredity. His model is based on the fact that alleles of a trait segregate from each other and are randomly distributed over the gametes during gametogenesis. Law of Segregation: Alleles segregate randomly during gametogenesis. As we will study later (Bio185), the segregation behavior of the alternative alleles is rooted in the alignment and separation of homologues chromosomes during meiosis I. Her207

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31 De Graaf Van Leeuwenhoek

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33 Lamarck

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61 Morgans first cross.

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73 Independent segregation

74 Linkage and cross-over.

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79 Co-dominant traits. ABO blood group system.

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81 Co-dominant traits: Piebald in animals.

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84 Jimsonweed (Datura stramonium) Incomplete dominance.

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90 Q. A cross of a chicken with a walnut comb with a chicken with a single comb yields equal proportions of offspring with a walnut, rose, pea, or single comb.

91 R-pprrP- R-P-rrpp New phenotypes result when more than one locus affects a trait.

92 A-B- aabb A-bb aaB-

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95 O-B- (natural)ooB- (black) O-bb (orange) Oobb (albino) Corn snake

96 Flower color in foxglove M- D- --Mm -- --M- dd ww M- D- ww

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100 A-B- A-bb aaB-/aabb

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102 Q1. Adherents of the theory that holds that all life was preformed at the moment of creation and that successive generations of individuals were encased, one inside the other, in increasingly small versions of their adult selves are called

103 Q2. Antoon van Leeuwenhoek was a(n) ______________, whereas Reinier de Graaf was a(n) __________.

104 Q3. Kolreuter (1760) and Knight (1790) independently observed grandchildren of the same color as one of the grandparents. These observations could not be reconciled with the __________ theory.

105 Q4. The fact that a man who lost both legs to amputation does not have leg-less children pleads against the ________________.

106 Q5. The AABbcc individual is haploid, diploid, triploid, or hexaploid.

107 Q1. How many traits are defined by the genotype AaBbCc?

108 Q2. How many traits are involved in a cross that yields 27 red, long- haired and 9 black, straight-haired kittens?

109 Q3. The eye color of fruit flies can be black, violet, brown, sepia, red, orange, peach, yellow, or white. Any given fruitfly carries at most _______ different eye color alleles.

110 Q4. If the genotype of an individual is MMOOpp what is(are) the genotype(s) of the gametes?

111 Q5. In peas purple is dominant over white. What is the probability that a test cross yields a white-flowered offspring? (= What is the proportion of white-flowered offspring among the offspring?)

112 Q1. How many different gametes are produced by an individual with genotype YyWwSsXx?

113 Q2. How many different phenotypes and genotypes are among the offspring of the test cross of a parent with genotype YyWwSs?

114 Q3. If yellow > green, smooth > wrinkled, and large>small, what is the probability that an offspring of a trihybrid cross will be large, yellow, and wrinkled?

115 Q4. If blue and white are alternatives of the same trait, what is the most likely genotype of the parents that produced 865 blue and 735 white offspring?

116 Q5. In peas, yellow>green and smooth>wrinkled. What cross (genotype of parents) yields the following phenotypic ratio of offspring? yellow, smooth3 yellow, wrinkled3 green, smooth1 green, wrinkled1

117 Q1. What is the probability of offspring of a trihybrid cross being heterozygous for each trait ?

118 Q2. The ABO-bloodgroup trait has three alternative forms: A, B, and O. O is recessive to A and B but A and B are co-dominant. What cross yields all possible ABO phenotypes?

119 Q3. If all traits show complete dominance, what are the degrees of freedom when you attempt to explain the results of a mating with a tri-hybrid cross?

120 Q4. The bloodgroup trait M has two co-dominant alleles, M and m. A cross yields 8 MM, 21 Mm, and 11 mm individuals. The coresponding model predicts 10 MM, 20 Mm, and 10 mm offspring. Write out the terms of 2 ?

121 Q5. The 2 -test of a cross yields P>0.05. What is P? P is the probability that A.I am correct by chance alone when accepting the model. B.the model explains the cross by chance alone. C.Chi-square is calculated correctly by chance alone. D.the offspring are obtained in such a cross by chance alone. E.the differences between the observations and expectations are due to chance alone.

122 Q2. What is the probability that the offspring of a trihybrid cross will have the genotype aaB-cc?

123 Q1. (a) What is co- dominance? Q1. (b) What is epistasis?

124 Q2. (a) If both traits show incomplete dominance what is the number of phenotypes among the offspring of a dihybrid cross? Q2. (b) If both traits show incomplete dominance, what is the most abundant phenotype among the offspring of a dihybrid cross?

125 Q3. If the shape of radishes can be round, oval, and long (incomplete dominance) what is the genotype of the parents that yields ¼ round, ½ oval, and ¼ long offspring?

126 Q4. Plum color is determined by two alleles with incomplete dominance and can be red, yellow, or green. What cross of two differently colored parents will never yield red fruits?

127 Q5. In rabbits short-hair > long-hair and black > blue. What cross yields 1 blue and 3 black, long-haired bunnies.

128 Q6. Jimson weed can be round, oval, long and smooth, fuzzy, or spiked. A cross of which 25% of the offspring are fuzzy, oval plants is a __________?

129 Q. A cross of a chicken with a walnut comb with a chicken with a single comb yields equal proportions of offspring with a walnut, rose, pea, or single comb.

130 Q3. To explain the results of a cross by way of a test cross 2 test yields P=0.03. What do you conclude?

131 Q4. What is the formula of 2 ?

132 Q5. What are the degrees of freedom for a 2 test using a dihybrid cross of one trait with incomplete dominance and one trait with codominance?

133 Q5. Use the pink sheet. For six comparisons you calculate a - square = What is P?

134 Q1. Alcohol dehydrogenase of honey bees protects against alcohol poisoning. Adhfast moves rapidly and far during electrophoresis; Adhslow moves slowly. In a monohybrid cross, half of the offspring has both Adhfast and Adhslow. The Adh is a ________ trait.

135 Q3. To explain the results of a cross by way of a test cross the 2 test yields P=0.1. What do you conclude?

136 Q4. What are the degrees of freedom for a 2 test using a test cross involving one incomplete, one co-, and one complete dominant trait?

137 Q1. A cross yields 9 chicks with leg feathers and 3 chicks without leg feathers. (a)What one-trait cross explains this result? (b)What two-trait cross explains this result?

138 Q2. The length of cat whiskers is a polygenic trait, according to the length of their whiskers cats can be grouped into 7 classes. How many traits contribute to the length of whiskers?

139 Q3. The eye color of fruit flies can be black, sepia, violet, burgundy, red, vermillion, orange, peach, or white. Eye color is a ___________ trait. a. Polygenic. b. Polymorphic. c. Pleiotropic. d. Epistatic. e. Co-dominant.

140 Q4. A cross yields 865 red and 735 white flowers. (a)What one-trait model best explains the cross? (b)What two-trait model best explains the cross?

141 Q5. Why do you use a -square test?


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