Presentation on theme: "TILAPIA PRODUCTION IN BIOFLOC SYSTEMS"— Presentation transcript:
1 TILAPIA PRODUCTION IN BIOFLOC SYSTEMS YORAM AVNIMELECHTECNION, ISRAEL INST. Of TECHNOLOGY
2 Biofloc technology is based upon a few principles:(a) Zero or minimal water exchange,(b) subsequent development of dense microbial population(c) managing the microbial population as a part of the pond eco-system(d) adjustment of the C/N ratio to 15 in order to control inorganic nitrogen concentration in the water.The bacteria, forming bioflocs, assimilate TAN, produce microbial proteins that is consumed by the fish, thus recycle the unused feed protein. (For more details: Yoram Avnimelech, Biofloc Technology, A Practical Handbook, World Aquaculture Soc. 2012).
4 Tilapia, an ideal fish for Biofloc Technology Grow well in dense culturesResistantFILTER FEEDERVery few studies as compared with shrimp
5 Fish Biomass Normally, 20-30 kg/m2 10 times higher than shrimp BFT ponds!
6 Implications: High Biomass 20-30 kg/m3 (Up to more than 40kg/m3 (w/o O2)High feeding (ca 500 g feed/m3 per day)Very high microbial activityHigh floc volume (20-50 ml/l).Very high natural feed storage.High levels of feed residuesNeed to drain out daily (or twice daily) excessive sludge.Pond constructed to facilitate sludge draining and perfect mixing.High and effective aeration: hp/1000 m2 pondImplications:
7 Drain bottom sludge twice daily till you get clear water
11 200 µmx10x20200 µm200 µmx10x10Bio flocs are made of bacteria, protozoa, etc. Typicaly their diameter is mm.
12 Protein Recycling Protein is the most expensive part of the feed. Normally, fish or shrimp recover just ~25% of feed protein.In bacterial controlled ponds, they eat the protein twice; Once in the feed and then they consume microbial protein. The protein recovery reaches almost 50%.Protein is the most expensive part of the feed.Protein Recycling
15 Residence time of bioflocs Bioflocs were taken up by fish and degraded biologically. Yet, the amount of bioflocs stayed almost constant. This implies that new flocs are constantly produced (using the excreted N).The residence time of bioflocs was calculated to be around 8 hours. The flocs seem to be a very dynamic system.most cells in the flocs are young and active.Residence time of bioflocs
16 Data on feed protein utilization Conventional fish, shrimp ponds %BFT Tilapia ponds (Avnimelech) %BFT Shrimp ponds (McIntosh) %Closed shrimp tanks (Velasco) %BFT shrimp ponds, 15N study 18-29% of total N consumption (Michele Burford et al.)* Tilapia, 15N Study, flocs supplied about 50% of fish protein requirement. (Avnimelech).Data on feed protein utilization
17 Fish or shrimp growing in BFT systems eat the pellets when applied, but eat bioflocs all the time. Confirmed in a work done by Albert Tacon with shrimp.Observations with tilapia.Effects on lowering of FCR in tilapia productionFeed Utilization
20 Effects on propagation Shrimp broodstock grown in BFT have earlier sexual maturityAnd higher productivitySimilar result with tilapiaWork by Julie EkasariBetter nutrition? Fatty acids?Hormonal effects?
21 Summary of fatty acids analysis on the collected bioflocs % Total Fatty AcidsTotal n-31.33Total n-619.70Total n-912.97LA13.43ALA0.36EPA0.56DHA0.32AA1.30n-6 fatty acids is essential for tilapia and its reproduction (Watanabe 1982)Protein : 37 – 46% DWLipid : 11.9 % DWProtein content of bioflocs collected from the BFT tank was ranged from 37 to 46%, whereas lipid level was around 11%.Fatty acids analysis of the bioflocs on day 84 showed that this bioflocs was rich in n-6 fatty acids which is considered as essential fatty acids for tilapia (Watanabe 1982).Previous studies showed that tilapia provided with diets containing vegetable oil rich in n-6 (soybean oil and CPO) resulted in higher reproductive performance than that of fish oil (Watanabe 1982; Ng & Wang 2011).It is also important to note that this bioflocs contained considerably high concentration of arachidonic acid (AA) which is a highly unsaturated fatty acids which plays an important role in fish fertilization success by stimulating testicular testosterone in goldfish (Izquierdo et al 2001)Biofloc considerably high in AA (Arachidonic Acid)AA is beneficial for fertilization rates stimulates testicular testosterone in goldfish (Izquierdo et al 2001)
22 What triggers the fish to spawn? (Rocha 2008) 2. Physiological factorsHormonesMorphological changesMobilization of energy reservesBlood total cholesterol in fish in BFT tanks was higherCholesterol precursor of steroids hormonesBlood glucose as an indicator of mobilization of energy reservesFish growthThe second factor which affect the reproductive efficiency in fish is physiological factor which include hormones, morphological changes and mobilization of energy reserves.In the present study, we measured blood total cholesterol and glucose concentration as the indicator for physiological factors related to reproductive activity.Cholesterol is known as a precursor for the biosynthesis of steroid hormones, including those which function in reproduction such as testosterone, estrogens, and progestogens (Lubzens, Young, Bobe & Cerda, 2010), whereas blood glucose can be used as an indicator of energy reserves mobilization during reproductive period.
23 Results: Blood total cholesterol Higher concentration in BFT higher concentration of hormones were involved?It is shown in this graph that blood cholesterol levels of the fish in BFT tanks were consistently higher than those in the Control tanks.Higher levels of blood cholesterol in BFT treatment can be supportive to improved reproductive performance of broodfish in this treatment.The higher concentration might also indicate that there might be higher concentration of reproductive hormones were involved which eventually resulted in a better reproductive performance of the fish in BFT?.This we believe can be interesting to be further investigated.Low total cholesterol level in the blood (less than 180 mg.dL-1) compared to a previous study (Chen et al 2003) could be associated with the fact that the fish had been undergoing spawning activities (McDonald & Milligan, 1992).
24 Results: Blood glucose Higher concentration in BFT More energy was mobilized for reproductive activitiesMore energy supply?Blood glucose levels in the fish in BFT treatment were also found to be constantly higher than those of the control, and the difference were significant, specifically on day 42 and 56.Blood glucose values may designate higher energy mobilization and also energy supply for the fish in BFT tanks during their reproductive period.
25 Results: Average body weight Despite of more active reproductive performance the female in BFT tanks grew betterYet, the fish in BFT required less feed(9.3 vs 8.3 kg/tank)The fish average body weight during the experimentation showed that despite of the higher reproductive performance, the fish in BFT tanks still grew better than those in the control tanks, and yet they consumed significantly less feed.
26 Results : Total fish fry production bTotal fish fry production per tank after 84 days of experimental periods in BFT treatment was 65% higher than that of the control and significant at P<0.05Julie Eskari
27 Fresh results from the field: Tilapia fingerlings (120 g) stocked to over-wintering facility on January 4, 2011.Feeding ~ 1% BWOn February 18, they weighed 171 gDaily growth of 1.16 g/fishFCR = 0.5Fresh results from the field:
30 11 (4.7) EFFECT OF WATER EXCHANGE RATE ON PRIMARY Injected fish AND SECONDARY INFECTION OF FISH BY STREPTOCOCCUSTotal infectedSick fishDead fishTreatment112 (2)9 (9)Control164 (2)12 (3)BFTControlInjected fishBFT(4.7)(1.5)(5)High exchange3 (1.4)1.3 (1.0)(1.7)ASPNon-injected fishControlBFTHigh water exchange = 0.5l/min per kg fish (700% per day)BFT< Limited water exchange = 10% per day
31 Luo and coworkers in China reported Yields of 45kg/m3. Higher production andlower FCR as compared with RAS.Higher concentrations of SuperoxidePermutase .
32 ConclusionsBiofloc technology is especially adapted to raise tilapia production up to kg/m2.This can be done using not too expensive system.BFT enables feed recycling, high feed quality and reduced expenses.BFT reduces disease.The system is friendly and forgiving.More research is needed
33 Cultivo de tilapia en sistemas biofloc es simple, fácil y rentable Cultivo de tilapia en sistemas biofloc es simple, fácil y rentable. • Tilapia como filtrador es feliz en esos sistemas. • Se ahorra los gastos de alimentación (~ 30%) y comer mejor. • Menos enfermedades. • Mayor crecimiento y menor FCR. • La manera más rápida de iniciar la producción intensiva de tilapia. • Si necesita ayuda, estaré feliz de hacerlo: