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Rapid methods for feed safety: the CONffIDENCE project Rapid Methods Conference & Open Day 25- 27 January 2010 www.conffidence.eu.

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Presentation on theme: "Rapid methods for feed safety: the CONffIDENCE project Rapid Methods Conference & Open Day 25- 27 January 2010 www.conffidence.eu."— Presentation transcript:

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2 Rapid methods for feed safety: the CONffIDENCE project Rapid Methods Conference & Open Day January 2010

3 CONffIDENCE in a nutshell CONtaminants in food and feed: Inexpensive DEtectioN for Control of Exposure

4 CONffIDENCE, a European approach

5 CONffIDENCE passport FP7 Collaborative Project first call Food, Agriculture & Fisheries, and Biotechnology Duration: May 2008 – April partners from 10 countries, representing universities, research institutes, industry and SMEs Budget: 7.5 Mio Co-ordinator: RIKILT - Institute of Food Safety, part of Wageningen UR (NL)

6 Why CONffIDENCE ? To assure chemical safety and quality in the European food supply; support of EC policies and competitiveness of food and feed industries

7 Why CONffIDENCE ? To improve multi-detection (multiplex) possibilities, e.g. for antibiotics

8 Why CONffIDENCE ? To improve inexpensive screening possibilities, e.g. for metal speciation Inorganic arsenic Arsenous acid As(III) Arsenic acid As(V)

9 Why CONffIDENCE ? To speed-up analysis for factory approval of lots

10 Why CONffIDENCE ? To contribute to the assessment of risks of emerging contaminants e.g. plant toxins such as pyrrolizidine alkaloids

11 Why CONffIDENCE ? To contribute to the generation of data for exposure assessment, e.g. for PFCs: Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and their salts Scientific Opinion of the Panel on Contaminants in the Food chain Adopted on 21 February 2008 Due to the substantial lack of suitable analytical data, many assumptions have been made in order to derive exposure estimates.

12 The objectives Development and validation of new simplified inexpensive detection methods for chemical contaminants from farm to fork

13 The objectives Improved exposure assessment through monitoring of selected contaminants

14 The objectives Contribute to validation of predictive hazard behaviour models

15 The objectives Dissemination and training of new detection methods to all relevant stakeholders, to advance technology exploitation

16 Food&Feed Fish/shellfish Fish feed CerealsCereal-based feed Potatoes/vegetables Honey Eggs Meat Dairy products The commodities Based on their relevance for consumer safety and trade, the following target commodities were selected:

17 The target contaminants Persistent Organic Pollutants (POPs): - dioxin-like PCBs - brominated flame retardants - polycyclic aromatic hydrocarbons (PAH) The targeted analytes represent a balance of emerging contaminants, high frequency detected residues as well as compounds which are not satisfactorily covered by current analytical methods.

18 The target contaminants Persistent Organic Pollutants (POPs) Perfluorinated compounds (PFCs): - PFOS - PFOA The targeted analytes represent a balance of emerging contaminants, high frequency detected residues as well as compounds which are not satisfactorily covered by current analytical methods.

19 The target contaminants Persistent Organic Pollutants (POPs) Perfluorinated compounds (PFCs) Pesticides: - paraquat - diquat - dithiocarbamates The targeted analytes represent a balance of emerging contaminants, high frequency detected residues as well as compounds which are not satisfactorily covered by current analytical methods.

20 The target contaminants Persistent Organic Pollutants (POPs) Perfluorinated compounds (PFCs) Pesticides Veterinary drugs: - antibiotics, e.g. tetracyclines - coccidiostats, e.g. ionophores The targeted analytes represent a balance of emerging contaminants, high frequency detected residues as well as compounds which are not satisfactorily covered by current analytical methods.

21 The target contaminants Persistent Organic Pollutants (POPs) Perfluorinated compounds (PFCs) Pesticides Veterinary drugs Heavy metals speciation: - inorganic arsenic - methyl mercury The targeted analytes represent a balance of emerging contaminants, high frequency detected residues as well as compounds which are not satisfactorily covered by current analytical methods. Inorganic arsenic Arsenous acid As(III) Arsenic acid As(V)

22 The target contaminants Persistent Organic Pollutants (POPs) Perfluorinated compounds (PFCs) Pesticides Veterinary drugs Heavy metals speciation Biotoxins: - plant alkaloids: pyrrolizidine, tropane, ergot - marine biotoxins (PSP, DSP) - mycotoxins: DON, ZEA, T-2/HT-2, FB1, FB2 The targeted analytes represent a balance of emerging contaminants, high frequency detected residues as well as compounds which are not satisfactorily covered by current analytical methods. Ergot bodies

23 The technologies Bio-analytical techniques MS-based techniques Spectroscopic techniques

24 Bio-analytical techniques Analyte Bio-molecule Platform

25 Bio-analytical techniques Antibodies Receptors Transport proteins Aptamers Dipsticks SPR Flow cytometry Electrochemical immunosensors Cytosensors Bio-moleculePlatform

26 The technologies Multiplex dipsticks assays Multiplex SPR biosensor Multiplex flow cytometry Electrochemical immunosensors Cytosensors D) Chloramphenicol C) Tylosin B) Quinolones A)Sulfonamides Transcription Translation mRNA Reporter plasmid P Luciferase operon Luciferase Luminescence R P = promoter R = regulatory protein Bio-analytical techniques

27 MS-based techniques Simplified mass spectrometric (MS) methods LC-MS or LC-MS/MS DESI-MS MS-based techniques

28 Near Infrared (NIR) imaging methods (nombre de pixels en x) (nombre de pixels en y) (Wavelength nm) Spectroscopic techniques

29 Project structure WP1a Persistant organic pollutants (POPs) Fish, fish feed and cereal based food Flow cytometry based multiplex assay GCxGC-TOF-MS comprehensive screening ICT-RIKILT-JRC IRMM-NUTRECO WP1b Perfluorinated compounds (PFCs) Fish, fish feed and dairy products Simplified LC-MS method CSIC-ICT-JRC IRMM-NUTRECO WP1c Pesticides (Paraquat, Diquat) Potato and cereals Validated electrochemical magneto immunosensor assay DESI-MS / DART-MS (dithiocarbamates) RIKILT-CSIC-FERA-CVUA-ICT WP2a Coccidiostats Eggs, Laying hens feed Flow cytometry based multiplex immunoassay JRC IRMM-CER-RIKILT-QUB- NUTRECO-FERA WP3 Heavy metals Marine based feed and food Cytosensor and solid phase extraction approaches DTU Food-TUT-JRC IRMM- NUTRECO-RIKILT WP2b Antibiotics Fish, feed, meat, honey Single component and multiplex dipstick Electrochemical magneto immunosensor assay FERA-UNISENSOR-CER-CSIC- NESTLE-JRC IRMM-NUTRECO WP4a Alkaloids (pyrrolizidine, tropane and ergot) Honey, feed and cereals Validated multiplex dipstick assay On-line imaging method RIKILT-QUB-CRAW-UNISENSOR- FERA-CER-NUTRECO WP4b Marine biotoxins Shellfish Rapid multiplex SPR biosensor assay QUB-USC-CER-JRC IRMM WP4c Mycotoxins Cereals for food and feed Validated multiplex dipstick ISPA-UNISENSOR-CER-RIKILT- NUTRECO-NESTLE

30 Project structure

31 Dissemination: the website

32 Website: some statistics (Google Analytics)

33 33 events on-line 15 news on-line 3 newsletters delivered 14 links to related projects Publications 1 Open Day To be informed, registration on-line 48 stakeholders registered Information for stakeholders

34 First results Multiplex immunoassay for coccidiostats

35 First results flow cytometry Calibration curves of nicarbazin, diclazuril, salinomycin and narasin in buffer Sensitivities of nicarbazin, diclazuril and salinomycin assays promising Multiplex immunoassay for coccidiostats nicarbazin salinomycin narasin diclazuril

36 First results Multiplex immunoassay for coccidiostats Tetracycline Tetrasensor assay for feed

37 - Pig FEED - dilution (10x) in buffer - Homogenisation (2 min) - Centrifugation (2 min) µl of supernatant + reagents - Test : 10 min at RT

38 First results Multiplex immunoassay for coccidiostats Tetracycline Tetrasensor assay for feed Ergot detection in cereals by NIR imaging

39 Barley sample including an ergot body Image rebuilt at 1200 nm after 1 st Derivative preprocessing

40 Ergot detection in cereals by NIR imaging Discrimination Ergot/Wheat based on 2 wavelengths (1220 nm/ 1440 nm)

41 Acknowledgements The CONffIDENCE project is financially supported by the European Commission under Grant Agreement no

42 Acknowledgements

43 More information Website: Contact: e-newsletter (registration on website)


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