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Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2.

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Presentation on theme: "Glucosyltransferases Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2."— Presentation transcript:

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3 Glucosyltransferases

4 Alternan Mutan Dextran Glucosyltransferases Leuconostoc mesenteroides Streptococcus linkages 1-6 B-512F B-1299 B-1355 1-3 1-2 mutans GS5 downei Mfe28 Microorganismenzyme Dextransucrase Alternansucrase Mutansucrase Glucansucrase 1-6 1-6 1-3 1-6 1-3 1-6 1-3 strainpolymer Glucan

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7 Oligosacaridos -Fructo-oligosacáridos -Galacto-oligosacáridos Lactulosa -Gluco-oligosacáridos Xylo-oligosacáridos Proceso Síntesis Enzimática Hidrólisis Enzimática (inulina) Extracción de Soya Síntesis Enzim{atica Isomerización de lactosa Síntesis Enzimática Hidrólisis de Almidón Síntesis Enzimática Hidrólisis Enzimática (Hemicelulosa) Empresa Meiji Seika Refinería Tirlemontoise Calpis Yakult Nikken Kapau Meiji Seika Showa Sangyo BioEurope fuente: Monsan P. Biofutur, (6,7), 1993

8 Bioprospection AgavePozolPulque

9 Pozol 14 strains of Leuconostoc mesenteroides Cell-associated activity Identify as Leuconostoc citreum by analysis of 16S rRNA screening of glucosyltransferases activities FRUCTOSYLTRANSFERASE

10 o Fructose o o HO OH HO OH HO OH o HO o Sucrose Polymer synthesis (n) OO OO o HO HOCH 2 O O HO CH 2 OHCH 2 OH CH 2 2 2 OH HO O CH 2 2 OH HO O CH 2 HO o OH + Levan Glucose Acceptor reactions Fructosyltransferases HO o OH Glucose + Hydrolisis + HO o OH Glucose HO O OO Maltose HO O OO O O OH HO Fructoside

11 ß-2,1 linked fructosyl moieties with branching through ß-2,6 linkages (n) O o HO O CH 2 HO CH 2 2 OH O HO O CH 2 2 OH O HO O CH 2 OH CH 2 OH Inulin Levan ß-2,6 linked fructosyl moieties with branching through ß-2,1 linkages OO OO o HO HOCH 2 O O HO CH 2 OHCH 2 OH CH 2 2 2 OH HO O CH 2 2 OH HO O CH 2 HO (n)

12 Applications of fructans in food Technological properties –Taste improvement –Texturizing agent (mouthfeel) –Sugar and fat replacement –Filler/binder in tablets Nutritional properties –Soluble dietary fiber –Prebiotic and related health effects –Sugar and fat replacement –Low caloric value –Suitable for diabetics

13 Fructosiltransferasas Plantas Neoseries de levana LEVANA levana Neoseries de inulina INULINA levana NEOKESTOSA 1- KESTOSA BIFURCOSA SACAROSA 6-KESTOSA 6-SFT 1-FFT 6-SFT 6G-FFT 1-SST + Sac Modelo de la biosíntesis de fructanas en plantas (Vijn y Smeekens, 1999). ©SCIMAT 2003 Bacterias SACAROSAINULINALEVANA IS LS

14 Azucar Caña de azúcar Enzima Fructosiltransferasa Glucosa Fructosa FOS Glucosa + Fructosa Sacarosa =

15 1 tea spoon of NutraFlora 22 bananas 15 onions 383 cloves of garlic


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