2 VecTOR Malaria Sporozoite Antigen Panel Assay TMMalaria Sporozoite AntigenPanel AssayEntomological Sciences DivisionUSACHPPM-NorthFort George G. Meade, MDPrepared byThe use or mention of a private entity in this document is not to beconstrued or interpreted as an official federal endorsement of same.
3 Learning Objectives Given a VecTOR test kit know: components use role in surveillance programlimitations
4 Malaria Adult Mosquito Surveillance Plan Alternatives Do nothingFocus surveillance on suspected risk areasConduct tests in order to predict malaria risk
5 Malaria Surveillance Considerations VectorDoes it bite humansCan it transmit malaria to humansIs there a high vector abundanceEnvironmental FactorsUsually low to moderate illness level in endemic areasEpidemic outbreaks are likely when conditions are suitableOutbreaks can occur when climate is favorable for short periodsHostIs there immediate onset of disease (10-20 days) or latent disease manifestation (6-9 months - e.g. Korea)Is there low level immunity in host population
8 Malaria Identification & Distribution Identify potential malaria high-risk areasCollect and identify adult mosquitoesPerform screening with VecTOR kitFollow up with PCR confirmationDefine high risk areasInitiate targeted control operationsIntegrate into a GIS platform to facilitate an effective management programSuspected malaria high-risk areas can be identified by monitoring human populations (especially civilian) infected with malaria. Adult Anopheles mosquitoes from surveillance collections for identifying vector/disease control strategies can be assayed by ELISA to determine the mosquito infection rate for vivax malaria parasites. These data will establish baseline data and provide estimates of risk of malaria transmission.When integrated into the GIS system, malaria infection rates can be correlated with larval habitats, adult mosquito populations and human populations for developing predictions and trends in malaria transmission.
10 Malaria VecTOR Kit Rapid immunochromatographic assay for the qualitative detection of:Plasmodium falcipariumPlasmodium vivax 210Plasmodium vivax 247in infected mosquitoes
11 Malaria VecTOR Kit Results can enable PM organizations to: Continuously monitor mosquito vectorsFocus vector controlDeliver cost-effective disease monitoring
12 Malaria VecTOR KitIntended to be a component of the Entomological Collection Kit, Field (ECK, Field)Separate Bde PM Section (1)Division PM Section (3)ASMB PM Section (3)Medical Detachment Preventive Medicine (3)SF Group (1)Civil Affairs Fld/UW BNPublic Health Team (1)Civil Action Team (1)
13 Malaria VecTOR Kit Item: Test Kit, Malaria Detection (MAL-K020) NSN:Cage Code: 3M5F7; AAC: Local PurchaseUnit of Issue : Kit of 20 testsUnit of Price: $260.00$13.00 per test (<10 female An spp. Mosquitoes/test)Shelf Life: Stable to expiration date when stored at room temp (10-30 deg C or deg F)Sensitivity: 92%; Specificity: 98.1%Estimated limit of detection: sporozoites
14 DOD PEST MANAGEMENT MATERIEL LIST (OTHER THAN PESTICIDES) June 1, 2007Table of ContentsSection 1 - Hand Sprayer, Pesticide, Manually Operated, Compressed AirSection 2 - Duster, Pesticide, Manually CarriedSection 3 - Sprayer and/or Duster, Pesticide, Manually Carried, BackpackSection 4 - Sprayer, Hydraulic or Electric, Mist or Solid Stream, Pesticide, Pushcart MountedSection 5 - Sprayer, Hydraulic or Electric, Mist or Solid Stream, Skid/Frame MountedSection 6 - Sprayer, Hydraulic or Electric, Mist or Solid Stream, Pesticide, Trailer MountSection 7 - Pesticide Sprayer, Fog/Aerosol Generator (ULV/ULD), Manually CarriedSection 8 - Pesticide Sprayer, Fog/Aerosol Generator (ULV/ULD), Skid MountedSection 9 - Pesticide Sprayer, Aerial Application Unit, Liquid or Dry PesticideSection 10 – Insect Traps, includes light traps and insect bait boxesSection 11- Animal TrapsSection 12 - Rodent Bait StationsSection 13 - Granular SpreaderSection 14 - Pheromone Trap Stored ProductsSection 15 - Bird Pest Management Devices (Including bird traps)Section 16 - Sets and KitsSection 17 - MiscellaneousSection 18 - Personal Protection MaterielSection 19 - Devices for Electrocution of Flying InsectsAdministrative Procedures (including emergency requisition of pesticides)
16 Malaria VecTOR KitFor Deployment Support contact the Deployment Environmental Surveillance Program (DESP):Commander, USACHPPM5158 Blackhawk RoadATTN: MCHB-TS-RDEAberdeen Proving Ground, MDPhone: DSN ; FAX -2407COM (410) ; FAX -2407Manufacturer Direct Orders contact:VecTOR Test Systems, Inc.2033 Yellowthroat PlaceThousand Oaks, CA 91320Phone: (805) , FAX (805)
17 Malaria VecTOR Kit Specimen Collection and Preparation Use appropriate collection methods to capture female Anopheline species known to be malaria vectorsCollected Anopheles spp. should be immediately identified and tested or,If not immediately tested, stored at -20 deg C until they can be processed
18 Malaria VecTOR Kit Materials Provided Assay dipsticks (20) in canister with desiccant cap1 Bottle of grindingSolution20 Conical Tubes
20 Malaria VecTOR Kit Materials Required but Not Provided: Mosquito keys Mosquito Collection/Pooling Log SheetPermanent MarkerTiming DeviceWash bottleMotorized grinder (optional)
21 Malaria VecTOR Kit Materials Required but Not Provided: Forceps Latex glovesCoolers/gel packsWhite enamel or plastic sorting trayDissecting Scope w/light source
22 Malaria VecTOR Kit Grinding Solution: Before use: Mix gently, inverting the bottle five timesWhen dispensing drops:Hold the bottle in an upside-down, vertical positionSqueeze the white portion of the dropper cap just above the the tip.Holding the bottle at an angle will cause the size of the drops to vary, causing inconsistent solution dispensing
23 Malaria VecTOR Kit Procedure: Place 1-10 female Anopheline mosquitoes into conical grinding tubeLabel tube with pool numberPlace the tube in the standDispense 13 drops of grinding solution into the tube
24 Malaria VecTOR Kit Procedure Continued: Place pestle into the grinding tubeRotate it vigorously to grind up the mosquitoesWash the pestlebetween samples (tubes)
25 Malaria VecTOR Kit Procedure Continued: Remove test strip (dipstick) from containerReplace desiccant cap on canister to protect remaining test stripsLabel pool numberon the back side oftest stripPlace test strip intube labeled withcorresponding poolnumber
26 Malaria VecTOR Kit Procedure Continued: Wait 15 minutes for test to be complete – read results(Results should be read within 30 minutes. After 30 minutes dipsticks turn gray- and results can be obscured.)DO NOT read dipsticks after they dry.
27 Malaria VecTOR Kit Procedure: Determine test results Remove test strip Compare test strip to pictorial sample in the VecTOR Kit instructionsPresence of only a control line indicates a negative resultTest results are not valid if the control line does not develop
28 Malaria VecTOR Kit Procedure: Presence one or more lines (other than control) indicatesthe presence one or more malaria strains:Plasmodium falciparum, P. vivax 210, or P. vivax 247.
29 Malaria VecTOR Kit Dipsticks can be saved, taped to a sheet of paper with pertinentinformation added,as a record of assayresults
31 Malaria VecTOR Kit Limitations: Can not test more than 10 mosquitoes per testMosquito identificationID to genus vs. ID to speciesScreening ToolCan not be confirmed by PCR in a Laboratory from strip (additional mosquito material is needed)
32 Malaria VecTOR Kit Used pipette tips, dipsticks, and sample tubes can all be disposed of in regulartrash (all non-hazardous)
33 Malaria VecTOR KitPM Dets, ASMB PM Sec, Div PM Sec, Sep Bde PM Sec, SF Group PM, Civil Affairs TeamsAnother tool in your tool boxCan be used to test field collections of Anopheline mosquitoesHone your mosquito ID capabilityPractice Malaria vector surveillanceExpect low infection rates, unless a large outbreak occurs
35 Malaria VecTOR KitFor Deployment Support contact the Deployment Environmental Surveillance Program (DESP):Commander, USACHPPM5158 Blackhawk RoadATTN: MCHB-TS-RDEAberdeen Proving Ground, MDPhone: DSN ; FAX -2407COM (410) ; FAX -2407Manufacturer Direct Orders contact:VecTOR Test Systems, Inc.2033 Yellowthroat PlaceThousand Oaks, CA 91320Phone: (805) , FAX (805)
36 Malaria VecTOR Kit Item: Test Kit, Malaria Detection (MAL-K020) NSN:Cage Code: 3M5F7; AAC: Local PurchaseUnit of Issue : Kit of 20 testsUnit of Price: $260.00$13.00 per test (<10 female An spp. Mosquitoes/test)Shelf Life: Stable to expiration date when stored at room temp (10-30 deg C or deg F)Sensitivity: 92%; Specificity: 98.1%Estimated limit of detection: sporozoites
37 Using the Malaria Sporozoite Antigen Panel Assay Kit EXERCISE:Using the Malaria SporozoiteAntigen Panel Assay Kit
38 ObjectivesGiven field collected mosquitoes, a list of common mosquito genera, a mosquito key, a Malaria VecTOR Kit, DA Form 8011-R, Mosquito Pool Log Sheet, a dissecting microscope w/light source:Sort Anopheline mosquitoes from all other generaPool the Anopheline mosquitoesTest the pools using the VecTOR kit (malaria sporozoite antigen panel assay)
39 Malaria VecTOR PE CONDITIONS You are supporting general mosquito (vector-borne disease surveillance) at an OCONUS base camp. Given a country specific mosquito key, dissecting microscope w/light source, field collected mosquito specimens, DA Form 8011-R, Malaria lab pooling sheet, Malaria VecTest Antigen Panel Kit, permanent marker, and appropriate label information, prepare specimens for and test anopheline mosquitoes for malaria.
40 Malaria VecTOR PE STANDARDS 1. Identify required materials 2. Properly record collection data on DA Form R3. Sort Anopheline mosquitoes from all other genera4. Pool Anopheline mosquitoes by trap, by collection date; recordpool data on Malaria Pool Log Sheet5. Test Anopheline pools using the VecTOR KitFollow VecTOR Kit directions:Label vialsLabel dip sticks6. Record test results and archive test strips permit and/or labelingrequirements7. Pack and label pool specimens shipment for shipment to a CONUSLab
41 Properly record collection data on DA Form 8011-R