Presentation on theme: "NIBSC Multiplex Proficiency Panel - A multicentre study"— Presentation transcript:
1NIBSC Multiplex Proficiency Panel - A multicentre study Clare Morris and David PadleyDivisions of Retrovirology and Virology
2Panel HistoryPanels produced for the UK National Blood Transfusion Authority.UK requirement to screen for HCV RNA in blood donations since 1999.Initially, annual HCV proficiency panels produced to monitor assay and operator performance across UK sites.In 2005 HIV-1 screening introduced in the one NBS site.1st HIV-1 RNA Proficiency panel produced in 2006.Panels have consisted of dilutions of a single marker, up to 15 members/panel.
3New multiplex approach Panel consisted of 15 coded samples.Contained combinations of HIV, HCV, HBV.One plasma negative was included.7 labs took part, 2 labs reported data using two systems.Due to potential for confirmatory testing each lab was sent 4 panels.Requested to test panel through routine algorithm.5 labs used commercial assays, 2 used in house assaysBiomerieuxAmplicorProcliexUltrioIn house132
7+ 4a 4b 7a - 7b HBV results Not tested for HBV Reactive LAB Sample 1 HCV234HIV567NEG8HCVHBV910HCV + HIV1112HCV + HBV1314HIV + HBV15LAB+Not tested for HBV4a4b7aReactive-7b
8ConclusionsHIV: One lab didn’t detect 100IU in triplex sample. All other labs detected HIV at all concentrations.HCV: Lab 2 failed to detect 20 IU sample. Lab 6 failed to detect 10 IU sample & 100 IU in a triplex sample.HBV: 3 data sets available. All HBV samples detected.50 proficiency panels remaining, if you would like to receive one please E mail me at: