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MicroRNA-155 mediates downregulation of the high-affinity receptor for IgE through Toll-like receptor signaling  Nicole Leib, Dipl Biol, Nadine Herrmann,

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Presentation on theme: "MicroRNA-155 mediates downregulation of the high-affinity receptor for IgE through Toll-like receptor signaling  Nicole Leib, Dipl Biol, Nadine Herrmann,"— Presentation transcript:

1 MicroRNA-155 mediates downregulation of the high-affinity receptor for IgE through Toll-like receptor signaling  Nicole Leib, Dipl Biol, Nadine Herrmann, PhD, Susanne Koch, PhD, Tim J. Stroisch, MSc, Sylvia Schnautz, PTA, Helene Wilms, BTA, Thomas Bieber, MD, PhD, MDRA  Journal of Allergy and Clinical Immunology  Volume 141, Issue 1, Pages e6 (January 2018) DOI: /j.jaci Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Ectopic miRNA-155 decreases PU.1 and FcεRI expression. CD34LCs were left untreated (Ø), were treated with P3C, or were transfected with the indicated pre-miRNA for 24 hours. A, Mean ± SEM values of stimulation experiments (n = 7) are shown. B-F, miRNA expressions (Fig 1, B) and mean ± SEM values of miRNA levels (Fig 1, C), mRNA (Fig 1, D and E), and relative fluorescence index (rFI; Fig 1, F) of transfected cells (n = 8-10) are depicted. G, Representative histograms of FcεRI protein expression are shown. n.s., Not significant; RQ, relative quantitation. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Different TLR stimuli were able to decrease PU.1 and FcεRI expression. CD34LCs were left untreated (Ø) or were treated as indicated and analyzed by using TaqMan MicroRNA Assays and quantitative PCR. A and B, Mean ± SEM values of miRNA-155-5p, mRNA levels of PU.1 and FCER1A, and relative fluorescence index (rFI) of FcεRI from 5 to 8 experiments are shown. S.a., Staphylococcus aureus; S.p., Streptococcus pyogenes. C, Pearson correlations and correlation significances of Δ cycle threshold values of PU.1, FCER1A, and miRNA-155 of stimulation experiments are depicted. *P < .05. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 miRNA-146a expression does not correlate with PU.1 and FCER1A expression. Pearson correlations of Δ cycle threshold values of PU.1, FCER1A, and miRNA-146a of stimulation experiments from Fig 2 are shown. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 miRNA-155 downregulates myeloid differentiation primary response gene–88 (MYD88). CD34LCs were transfected with indicated pre-miRNA for 24 hours. Mean ± SEM values of MYD88 mRNA levels of 7 to 9 experiments are shown. n.s., Not significant; RQ, relative quantitation. *P < .05. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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