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Toxicologie de nanoparticules métalliques à de faibles concentrations, pertinentes du point de vue environnemental CNRS, UMR EPOC 5805 Université de Bordeaux.

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Presentation on theme: "Toxicologie de nanoparticules métalliques à de faibles concentrations, pertinentes du point de vue environnemental CNRS, UMR EPOC 5805 Université de Bordeaux."— Presentation transcript:

1 Toxicologie de nanoparticules métalliques à de faibles concentrations, pertinentes du point de vue environnemental CNRS, UMR EPOC 5805 Université de Bordeaux Station marine dArcachon par Jean-Paul Bourdineaud professeur, Université de Bordeaux 1 er CONGRÈS SANTÉ-ENVIRONNEMENT Organisé par lURPS-Médecins libéraux de PACA 6 & 7 Décembre 2013 Marseille - Palais du Pharo

2 Comment des nanoparticules peuvent-elles être introduites dans un système écologique ? Quelle peut être lampleur de la pollution ?

3 Kaegi et al., Environmental Pollution, 156 (2008) Fresh facade painting Older facade painting Collected particles from runoff events Concentration of micro and nanoparticles in rain runoffs

4 Les nanoparticules peuvent-elles être distribuées dans les différents compartiments dun système écologique ? Et si oui, quel est le niveau de transfert vers les différentes composantes biologiques ?

5 (Ferry et al.) A single dose, 7.1 x of gold nanorods/L (65 x 15 nm), making 157 g Au NPs/L.

6 Genotoxic impact of metal nanoparticles on the zebrafish Danio rerio Gold nanoparticles are used in pregnancy tests, toothpaste, energizing creams and beverages, detection and treatment of cancer tumours. Cadmium sulfide nanoparticles are used in electronic devices. MesoGold, 20 ppm colloidal gold, 250 mL, by Purest Colloids Price: $29.97 Fulfilled by Amazon (Gift-wrap). Supports mental focus & concentration, and improved hand/eye coordination. Promotes improved memory & a general feeling of well being.

7 Impact of cadmium sulfide and gold nanoparticles on the zebrafish Controls CdS (10 nm)CdS (50 nm) Au (12 nm)Au (50 nm) Contamination through diet (5 µg nanoparticles/g food) giving 40 ng nanoparticles/fish/day Exposure length: 60 days 100 nm Nanoparticles embedded in the food 12 nm NPs 50 nm NPs

8 Metal quantification (cadmium and gold) in zebrafish tissues after 60 days of exposure. Gold could not be quantified in tissues and even in whole fish. Detection limits by atomic absorption spectrometry : Cadmium : 0,1 µg/L Gold : 1 µg/L Diet LiverBrainMuscles Whole fish Control food 8 ± 89 ± 0,90,6 ± 0,1 32 ± 8 CdS (10 nm) 348 ± 3019 ± 31,8 ± ± 10 CdS (50 nm)364 ± 3010 ± 33,1± 1 50 ± 5 Liver is the organ accumulating the most cadmium The little nanoparticles are more accumulated than the bigger in the brain and at the scale of the whole fish. Total Cd in ng/g

9 Differential gene expression after 60 days of exposure sod1sod2mt 2rad 51c-junp53baxpyccox1 Liver Au (12 nm)1/181/3811/711/101/271/451/201/91/12 Au (50 nm)/1/28//1/71/161/4// CdS (10 nm)/1/712331/6/1/2/1/4 CdS (50 nm)1/31/23 /1/6/1/5 / Brain Au (12 nm) /9 Au (50 nm) /5//56/41/38 CdS (10 nm) /225//2/1/5/ CdS (50 nm) 333/10/27/ Muscle Au (12 nm)////1/2//9/ Au (50 nm)///1/33/ 1/61/16 CdS (10 nm) // CdS (50 nm)/0,4/2////1/15 Oxidative stressDetoxication DNA repair General stress Mitochondrial metabolism 1/ CdS and gold nanoparticles trigger a different gene expression pattern. 2/ Size does matter !

10 Control DNA Contaminated DNA Pollutant Probe OPB OPB Loss of hybridization site Principle of RAPD (Random amplified polymorphic DNA)

11 OPB Principle of RAPD (Random amplified polymorphic DNA) OPB Control DNA Creation of hybridization site Contaminated DNA

12 Quantification of genotoxic damages by RAPD-PCR methodology RAPD probeTémoinsCdS (10 nm)CdS (50 nm)Au (12 nm)Au (50 nm) OPB7 0,6 ± 0,10,7 ± 0,1* 6,5 ± 0,9* 8,5 ± 0,9* 1,9 ± 0,2 OPB11 (2,1 ± 0,7) * (31 ± 14) * (4300 ± 1800) * (5600 ± 1800) * (280 ± 70) / The numbers of hybridization sites for RAPD probes was modified by nanoparticles. 2/ The two metals presented different effects. 3/ Size still matters ! Genomic DNAs were extracted from whole zebrafish individuals Number of hybridization sites per genome of the RAPD probe RAPD probes sequences: OPB7 : 5-GGTGACGCAG-3 OPB11 : 5-GTAGACCCGT-3 n = 10, mean ± SEM

13 Les nanoparticules piégées dans les sédiments aquatiques peuvent-elles être libérées dans la colonne deau et représenter un risque pour la santé des animaux ?

14 Impact of gold nanoparticles (14 nm) on zebrafish exposed to a spiked sediment Brain AChE activity (nmol/mg/min) Brain ache gene expression (relative to bactin gene) 440 ± ± 8551 ± ± ± ± 0.05 ControlsAu (16 mg/kg)Au (55 mg/kg) Exposition : 20 days 0.25 g/L0.8 g/L

15 Les nanoparticules incluses dans les sols peuvent-elles représenter un risque pour la santé des animaux ?

16 Metal nanoparticles trigger an oxidative stress in the earthworm Eisenia fetida Silver nanoparticles are used as antibacterial and antifungal agents in textiles, water treatments, and medical devices.

17 Impact of gold and silver nanoparticles on the red earthworm Contamination through the soil Exposure length: 10 days ControlsGold NPsIonic gold Silver NPsIonic silver 2, 10, and 50 mg/kg

18 Lipid peroxidation and protein oxidation in earthworms exposed for 10 days to ionic and nanoparticular god and silver Lipid peroxidationProtein carbonylation MDA : malondialdehyde CH 2 CC H H O O

19 Counter-adaptive levels of catalase in earthworms exposed for 10 days to ionic and nanoparticular gold and silver


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