1. (A) Schematic representation of the conditional Lats2 locus.
(A) Schematic representation of the conditional Lats2 locus. Upon mammary-specific CRE expression, exon 5 (colored blue) is deleted. (B) Genotyping of the Lats2 and the Cre alleles. Asterisks designate nonspecific bands. (C) Expression levels of Lats2 mRNA in WT-PyMT and Lats2-CKO PyMT tumors, quantified using RT-qPCR. Numbers of mice are indicated at the bottom; mean ± SEM; **P-value < (D) Representative H&E-stained sections of tumors from three month old Lats2-CKO PyMT and WT-PyMT littermate controls. Inset: immunohistochemical (IHC) staining for smooth muscle actin (SMA), a marker for myoepithelial cells surrounding the adenoma/MIN. Scale bar = 200 μm. (E) Kaplan–Meier plot of survival probability of luminal breast cancer patients (METABRIC, n = 1138) divided according to relative expression of the genes commonly down-regulated in LATS2L human lumB and Lats2-CKO PyMT tumors (n = 20, see Table S4).
Noa Furth et al. LSA 2018;1:e
© 2018 Furth et al.