38 The influence of storage conditions on the clonogenic capacity of periodontal cell : implication for tooth replantationP.C. Lekic , D.J. Kenny & E.J. BarrettInternational Endodontic Journal (1998)31,
39 INTRODUCTIONViable periodontal ligament (PL) cells are required for the healing of avulsed teeth after replantation.
40 INTRODUCTIONThe viability of PL cells in extra- alveolar conditions may be extended by incubating the avulsed tooth in a physiologic storage medium.
41 INTRODUCTIONRegeneration of PL following replantation is closely related to preservation of the viability PL cells that adhere to avulsed teeth
42 OBJECTIVESTo investigate the effects of combinations of storage media on the clonogenic capacity of human PL cells at two different extra alveolar period.
43 MATERIALS AND METHODS 20 human premolar teeth were extracted Aged 11 – 14 years4 storage media (saliva , milk , HBSS , MEM)All teeth were assayed at 30 and 60 min
44 MATERIALS AND METHODS Twenty extracted human premolars Time 0 min 15 teeth5 teethSaliva (23c)MEM (+4c)5 teethPer conditionMilkSalivaHBSSMEM (+4c)One-half of PL tissue explanted from premolar(cells released and analyzed for clonogenic capacity)
46 CONCLUSIONImmediate storage of a avulsed teeth in autologous saliva , a followed by transfer to chilled milk , preserves the presence of sufficient progenitor cells in the PL to warrant replantation and the possibility of PL healing at 60 min extra-alveolar duration.
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