The influence of storage conditions on the clonogenic capacity of periodontal cell : implication for tooth replantation P.C. Lekic, D.J. Kenny & E.J. Barrett International Endodontic Journal (1998)31,137-140
INTRODUCTION Viable periodontal ligament (PL) cells are required for the healing of avulsed teeth after replantation.
INTRODUCTION The viability of PL cells in extra- alveolar conditions may be extended by incubating the avulsed tooth in a physiologic storage medium.
INTRODUCTION Regeneration of PL following replantation is closely related to preservation of the viability PL cells that adhere to avulsed teeth
OBJECTIVES To investigate the effects of combinations of storage media on the clonogenic capacity of human PL cells at two different extra alveolar period.
MATERIALS AND METHODS 20 human premolar teeth were extracted Aged 11 – 14 years 4 storage media (saliva, milk, HBSS, MEM) All teeth were assayed at 30 and 60 min
MATERIALS AND METHODS Twenty extracted human premolars Time 0 min 15 min 30 min Saliva (23 c) MEM (+4 c ) Milk MEM (+4 c ) One-half of PL tissue explanted from premolar(cells released and analyzed for clonogenic capacity) SalivaHBSS 15 teeth 5 teeth Per condition
CONCLUSION Immediate storage of a avulsed teeth in autologous saliva, a followed by transfer to chilled milk, preserves the presence of sufficient progenitor cells in the PL to warrant replantation and the possibility of PL healing at 60 min extra-alveolar duration.
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