Presentation on theme: "Nucleic Acids DHPLC Applications in Biomedicine"— Presentation transcript:
1Nucleic Acids DHPLC Applications in Biomedicine In The Name of AllahNucleic Acids DHPLC Applications in BiomedicinePooria GillPhD of Nanobiotechnology
2Nucleic Acids DNA Linear RNA Nature-made Man-made Circular Vectors GenomicCytoplasmicrRNAMan-mademRNAtRNAOligo-primerncRNAOligo-probetmRNAQ-DNACircularsiRNAMitochondricOrganoplasmic
3ApplicationsDHPLC Systems (e.g. WAVE Systems) can be used for a wide range of applications including:Mutation DetectionSizingOligonucleotide Purification and AnalysisForensicsMicrobial AnalysisRNA Isolation and PurificationSingle Base ExtensionMethylation
4With the WAVE System you can: Scan fragments for both known and novel mutations/SNPs without extensive resequencingDetect low-abundance mutations in heterogeneous samplesEnrich for low-abundance allelesFractionate complex mixtures of related fragments based on differences in sequence contentPerform targeted mutation/SNP scoring via primer extension assaysAnalyze end-point products from allele-specific Probe/Primer-based Amplification Technologies
5Probe/Primer-based Amplification Technologies Primer-based Amplifications:Non-Isothermal Amplifications e.g. PCR-based MethodsIsothermal Amplifications/Non-PCR-based Amplifications:NASBAHDASDA, and more than 45 N.A.I.A. TechnologiesProbe-based Amplifications:For example: Ligase Chain Reactions
6CARTRIDGE dependent Applications e.g. via WAVE Systems DNA DNASep Cartridge 4.6 mm x 50 mm, for variety of DNA applications including Sizing and Mutation Detection, load capacity of 2µgDNA DNASep HT Cartridge 6.5 mm x 37 mm, for variety of DNA application but especially for Rapid Mutation Detection, load capacity of 4µgNUC OligoSep® Cartridge 4.6 mm x 50 mm; for Oligo Purification work onlyNUC OligoSep Prep HC Cartridge 7.8 mm x 50 mm, for Oligo Purification, load capacity of 5000µgRNA RNASep® Prep Cartridge 7.8 mm x 50 mm, for variety of RNA applications, load capacity of 50µg total RNA -OR- 1000ng of specific RNA
10Chromatograms and pedigree of patients 3 and 16. A Chromatograms and pedigree of patients 3 and 16. A. DHPLC analysis in normal control, patients 3 and 16. Both patients show abnormal DHPLC peak patterns with two peaks in comparison to normal control. B. Sequencing data in patient 3 and his family members. C. Sequencing data in patient 16 and his family members.