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1 Life Analytical Chemistry-Molecular Imaging (MI): Nuclear Imaging Gaolin Liang, Ph. D. Professor, Ph. D. Advisor Deptartment of Chemistry University.

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Presentation on theme: "1 Life Analytical Chemistry-Molecular Imaging (MI): Nuclear Imaging Gaolin Liang, Ph. D. Professor, Ph. D. Advisor Deptartment of Chemistry University."— Presentation transcript:

1 1 Life Analytical Chemistry-Molecular Imaging (MI): Nuclear Imaging Gaolin Liang, Ph. D. Professor, Ph. D. Advisor Deptartment of Chemistry University of Science and Technology of China

2 2 By Wei Huang, Ph.D. candidate Stanford University

3 3 By Gaolin Liang, Professor USTC

4 4 Nuclear Imaging PET SPECT Fusion techniques: PET/CT Fusion techniques: SPECT/CT Practical concerns in nuclear imaging The amount of radiation from diagnostic nuclear medicine procedures is kept within a safe limit and follows the "ALARA" (As Low As Reasonably Achievable) principle.

5 5 PET Positron Emission Tomography

6 6

7 7 Using 18 F, 11 C, 15 O, 64 Cu…for PET imaging Oxygen ( 15 O, T1/2 = 2 min); nitrogen ( 13 N, T 1/2 = 10 min); carbon ( 11 C, T 1/2 = 20 min); fluorine ( 18 F, T 1/2 = 110 min), copper ( 64 Cu, T 1/2 = 12 h) and iodine ( 124 I, T 1/2 = 4 days).

8 8 [18F]-fluoro-deoxy-glucose

9 9 Physiologic distribution of [18F]-FDG After i.v. injection, [18F]-FDG is distributed in the body like glucose. In accord with the glucose consumption of the cells, it is taken up by the glucose transport proteins, mainly by the GLUT-I, and phosphorylyzed by the hexokinase. Unlike glucose, [18F]-FDG does not take part in further steps of the glucose metabolism, which leads to a trapping of the molecule in the cells. Due to increased anaerobic glycolysis, many tumor cells show an up regulation of GLUT-I and of hexokinase, which together with physiologic trapping leads to an accumulation of FDG. [18F]-FDG is mainly excreted by the kidneys. In normal tissues, [18F]-FDG does not show a uniform distribution. The most intense physiologic uptake is seen in brain tissue, which is diagnostically used for a wide range of indications in neurology and psychiatry.

10 10 [18F]-FDG- HEAD AND NECK

11 11 [18F]-FDG- MYOCARDIUM

12 12 [18F]-FDG- GASTROINTESTINAL TRACT

13 13 [18F]-FDG- GENITOURINARY

14 14 [18F]-FDG- MUSCULAR ACTIVITY

15 15 [18F]-FDG- THYMUS UPTAKE

16 16 [18F]-FDG- BONE MARROW

17 17 [18F]-FDG- SPLENIC UPTAKE

18 18 [18F]-FDG- BENIGN PATHOLOGIC CAUSES OF 18F-FDG UPTAKE

19 19 Probes, applications, and instrumentations

20 20

21 21 18 F-SFB Synthesis

22 22 1 mL K 222 /K 2 CO 3 (15 mg/ml & 3 mg/ml in 9:1 ACN/H 2 O) drying 4-5 mg precursor, 1 ml anhydrous DMSO, 120 °C for 20 min Add 10 mL H 2 O, C 18 cartridge, wash once with 5 mL H 2 O 3 mL ACN (with 50 μL Bu 4 NOH) to rinse the cartridge 20 mg TSTU, 90 °C for 10 min HPLC purify, R t : min (7-37min: 5 – 65 % ACN) C 18 cartridge, wash it once (5 mL H 2 O) 1 mL ACN rinse the cartridge ( 18 F-SFB) Typical peptide labeling (Lys side chain): 10 mCi 18 F-SFB mg peptide Total time: 1 h Decay-corrected yield: 30-60% (5-10 peptides) Typical protein labeling (Lys side chain): 10 mCi 18 F-SFB mg protein Total time: min Decay-corrected yield: 1-30% (3- 5 proteins) Procedure

23 23 Liang, G.L.; et al. Unpublished results.

24 24 Xiaoyuan (Shawn) Chen, Ph.D. National Institute of Biomedical Imaging and Bioengineering (NIBIB)

25 25 Single Photon Emission Computed Tomography (SPECT)

26 26 Study Radioisot ope Emissio n energy (keV) Half-life Radiopharm aceutical Activit y (MBq)MBq Rotation (degrees) Projection s Image resolution Time per projection (s) Bone scan technetium -99m 1406 hours Phosphonate s / Bisphosphona tes x Myocardial perfusion scan technetium -99m 1406 hours tetrofosmin; Sestamibi Sestamibi x 6425 Brain scan technetium -99m 1406 hoursHMPAO; ECD x Tumor scan iodine hoursMIBG x 6430 White cell scan indium-111 indium-111 & technetium -99m 171 & hours in vitro labelled leucocytes x 6430 Wikipedia

27 27 Structures of Tc-99m probes MDP Sestamibi ECD BZM

28 28 SPECT/CT Qingqing Miao, Xiaoyu Bai, Yingying Shen, Bin Mei, Jinhao Gao, Li Li, and Gaolin Liang*. Intracellular self-assembly of nanoparticles for enhancing cell uptake. Chemical Communications. 2012, 48 (78),

29 29 SPECT/CT

30 30 SPECT/CT FOR SLN MAPPING

31 31 SPECT/CT IN SKELETAL DISEASES- Applications in Malignant Skeletal Diseases

32 32 SPECT/CT IN DIFFERENTIATED THYROID CANCER

33 33 SPECT/CT IN PARATHYROID TUMORS

34 34 SPECT/CT IN TUMORS OF SYMPATHETIC NERVOUS SYSTEM AND ADRENOCORTICAL TUMORS

35 35


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