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A What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can.

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Presentation on theme: "A What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can."— Presentation transcript:

1 a What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do?

2 a What happens inside? What else can Maxwell 16 do? Special Offer Special Offer Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do? Close What is Maxwell® 16? Imagine if your gDNA preps consisted of: Placing a sample into a cartridge Pushing a button Walking away Returning ~45 minutes later to high yield, pure gDNA ready for assay! The Maxwell 16 System is a cartridge-based purification system bringing automation to your benchtop. Change applications by changing cartridges and selecting a different program. Maxwell 16 can bring better results to your laboratory thanks to improvements in purity and consistency.

3 a What is Maxwell 16? What else can Maxwell 16 do? Special Offer Special Offer Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do? Close What happens inside? Maxwell® 16 is a magnetic particle processor, not a liquid handler. The sample is mixed by rapid up-and-down movement of the single-use plunger. The MagneSil® Paramagnetic Particles are captured after a magnetic rod insert down the middle of the plunger. The MagneSil Particles bind the nucleic acid The nucleic acids are purified through a series of capture and release washes The purified nucleic acid is released. Maxwell 16 ® Tissue Genomic DNA Cartridge Mixing Capture Binding Washing & Elution 300µl

4 a What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Special Offer Special Offer Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do? Close What else can Maxwell® do? Low Elution Volume (LEV) gDNA Standard Elution Volume (SEV) & LEV Total RNA SEV Polyhistidine Protein Purification SEV? LEV? SEV? LEV?

5 a What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Special Offer Special Offer Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do? Close What else can Maxwell® do? Low Elution Volume (LEV) gDNA Standard Elution Volume (SEV) & LEV Total RNA SEV Polyhistidine Protein Purification Close Maxwell® 16 can work with two formats of cartridges. SEV & LEV Standard Elution Volume (SEV) Low Elution Volume (LEV) Maxwell® 16 Instrument can switch from SEV to LEV format or back in less than 5 minutes µl µl

6 a What happens inside? What is Maxwell 16? Special Offer Special Offer Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do? Back Low Elution Volume gDNA Process µm formalin-fixed paraffin-embedded sections after overnight proteinase K digest. No xylene extractions. Maxwell ® 16 FFPE Tissue LEV DNA Purification Kit Cat. # AS1130 Maxwell ® 16 Cell LEV DNA Purification Kit Cat. # AS1030 Maxwell ® 16 LEV Total Nucleic Acid Purification Kit Coming Soon! Designed for 10-10,000 cells. Ideal for capturing gDNA from samples like stem cell colonies, cerebral spinal fluid, etc. Currently in testing for viral total nucleic acid purification More information in the Clinical Section of Close

7 a What happens inside? What is Maxwell 16? Special Offer Special Offer Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do? SEV & LEV Total RNA Maxwell ® 16 Total RNA Purification Kit Cat. # AS1050 Maxwell ® 16 Tissue LEV Total RNA Purification Kit Cat. # AS1220 Back Close Maxwell ® 16 Cell LEV Total RNA Purification Kit Cat. # AS1225 Maxwell ® 16 Tissue LEV 28S 18S M Brain 2 Heart 3 Kidney 4 Liver 5 Lung 6 Spleen 1µg loaded, denaturing gel Samples are homogenized & processed to remove gDNA prior to addition to the cartridge and purification.

8 a What happens inside? What is Maxwell 16? Special Offer Special Offer Plant Tissues & Seeds Plant Tissues & Seeds Cultured cells & bacteria What can Maxwell® 16 do? SEV & LEV Total RNA Maxwell ® 16 Polyhistidine Protein Purification Kit Cat. # AS1220 Back Close Purifies polyhistidine-tagged protein from bacteria, insect cells, mammalian cells, cell-free expression reactions, conditioned cell culture media, etc. TotalWashEluate HQ-tagged MonsterGreen Fluorescent Protein expressed in S30 T7 High Yield Expression System

9 B = Mouse Brain H = Mouse Heart I = Mouse small intestine L = Mouse Liver P = Mouse pancreas S = Mouse spleen T 1 = 1cm mouse tail T 0.5 = 0.5 cm mouse tail 5µl gDNA eluate 1µl gDNA eluate in PCR Quick Isolation from a variety of tissues Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS preps Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS preps Maxwell 16 ® Tissue DNA Purification Cartridge Tissue Sample Mouse tails and other animal tissue are homogenized directly in lysis buffer without Proteinase K. gDNA from Model Organisms gDNA from Food Samples Back

10 B = Mouse Brain H = Mouse Heart I = Mouse small intestine L = Mouse Liver P = Mouse pancreas S = Mouse spleen T 1 = 1cm mouse tail T 0.5 = 0.5 cm mouse tail 5µl gDNA eluate 1µl gDNA eluate in PCR Quick Isolation from a variety of tissues Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS preps Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS preps Maxwell 16 ® Tissue DNA Purification Cartridge Tissue Sample Mouse tails and other animal tissue are homogenized directly in lysis buffer without Proteinase K. Use Maxwell® 16 to speed research with Model Organisms More information online Close Source Yield (PicoGreen ® ) C t (1µl eluate) C t (5µl eluate)Target Drosophila melanogaster 0.32µg (1 fly) 1.52µg (5 flies) GAPDH gene Danio rerio 24.8µg 50mg tissue p53 gene Caenorhabditis elegans 0.08µg ~50,000 whole worms not detected 34.4Laminin Saccharomyces cerevisiae 0.72µg 3mm colony with lyticase pre- treatment LYS2 gDNA from Food Samples Back

11 B = Mouse Brain H = Mouse Heart I = Mouse small intestine L = Mouse Liver P = Mouse pancreas S = Mouse spleen T 1 = 1cm mouse tail T 0.5 = 0.5 cm mouse tail 5µl gDNA eluate 1µl gDNA eluate in PCR Quick Isolation from a variety of tissues Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS preps Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS preps Maxwell 16 ® Tissue DNA Purification Cartridge Tissue Sample Mouse tails and other animal tissue are homogenized directly in lysis buffer without Proteinase K. gDNA from Model Organisms Close Directly process food samples to detect… milkcheeses Panel A: S. aureus presence in milk and cheese samples Panel B: Multiplex PCR for S. aureus toxins …bacteria in dairy products Amplification of 18S bovine rRNA and 16S bacterial rRNA. …bacteria in raw meat samples Data courtesy of P. Leone and P. Cremonesi, Italian National Council of Research-Instititute of Agro-Biology and Biotechnology More information online: /applications Back

12 Maxwell ® 16 Blood DNA Purification Kit Cat. # AS preps Maxwell ® 16 Blood DNA Purification Kit Cat. # AS preps µl fresh whole blood +4°C -20°C RT Storage Condition Apoptotic DNA Isolate gDNA from good or bad blood Maxwell 16 ® Blood DNA Purification Cartridge Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge Buffy Coat Data Concordance Study Back

13 Maxwell ® 16 Blood DNA Purification Kit Cat. # AS preps Maxwell ® 16 Blood DNA Purification Kit Cat. # AS preps µl fresh whole blood +4°C -20°C RT Storage Condition Apoptotic DNA Isolate gDNA from good or bad blood Maxwell 16 ® Blood DNA Purification Cartridge Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge Concordance Study Close Using buffy coat cells gives higher gDNA yields and concentrations Buffy Coat gDNA >100ng/µl Back

14 Maxwell ® 16 Blood DNA Purification Kit Cat. # AS preps Maxwell ® 16 Blood DNA Purification Kit Cat. # AS preps µl fresh whole blood +4°C -20°C RT Storage Condition Apoptotic DNA Isolate gDNA from good or bad blood Maxwell 16 ® Blood DNA Purification Cartridge Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge Buffy Coat Data Close PCR results concordant to reference extraction method 16 samples amplified, analyzed by gel electrophoresis Data courtesy: Dr. Deborah O. Crowe, PhD, D(ABHI) DCI Laboratory, Nashville, TN Reference Manual Purification Method Maxwell® µl whole blood Maxwell® µl buffy coat cells

15 Quick gDNA isolation from cultured cells Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell 16 ® Cell DNA Purification Cartridge Directly process Cultured mammalian cells Gram negative bacteria Pre-treat Gram positive bacteria with lysozyme HeLa 1x10 6 cells Designed for 1 x 10 5 to 5 x 10 6 mammalian cells Gram Negative Bacteria Gram Negative Bacteria Gram Positive Bacteria Gram Positive Bacteria 3T3CHO293 Back

16 Quick gDNA isolation from cultured cells Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell 16 ® Cell DNA Purification Cartridge Directly process Cultured mammalian cells Gram negative bacteria Pre-treat Gram positive bacteria with lysozyme HeLa 1x10 6 cells Designed for 1 x 10 5 to 5 x 10 6 mammalian cells Gram Positive Bacteria Gram Positive Bacteria 3T3CHO293 Close Genomic DNA isolation from a variety of Gram negative bacteria 400µl overnight culture Escherichia coli Pseudomonas aeruginosa Proteus vulgaris Salmonella typhimurium Yersinia enterocolitica Designed to work with up to 2 x 10 9 bacterial cells

17 Quick gDNA isolation from cultured cells Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell 16 ® Cell DNA Purification Cartridge Directly process Cultured mammalian cells Gram negative bacteria Pre-treat Gram positive bacteria with lysozyme HeLa 1x10 6 cells Designed for 1 x 10 5 to 5 x 10 6 mammalian cells Gram Negative Bacteria Gram Negative Bacteria 3T3CHO293 Close Genomic DNA isolation from a variety of Gram positive bacteria 400µl overnight culture Staphylococcus aureus Arthrobacter luteus Bacillus subtilis Bacillus cereus Enterobacter faecalis Cells treated with lysozyme prior to isolation Designed to work with up to 2 x 10 9 bacterial cells 400µl overnight culture Back

18 Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps More Plant Samples More Plant Samples Maxwell 16 ® Tissue DNA Purification Cartridge Leaf Punch Many plant samples can be processed directly. Others may require an enzymatic pre-treatment Improve the purity and consistency of your plant genomic DNA purification Isolation genomic DNA from eight samples of 25mg each of tomato leaf using the Maxwell 16 Instrument and the Maxwell 16 Tissue DNA Purification Kit. Back

19 Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell ® 16 Cell DNA Purification Kit Cat. # AS preps Maxwell 16 ® Tissue DNA Purification Cartridge Leaf Punch Many plant samples can be processed directly. Other may require an enzymatic pre- treatment Improve the purity and consistency of your plant genomic DNA purification Isolation genomic DNA from eight samples of 25mg each of tomato leaf using the Maxwell 16 Instrument and the Maxwell 16 Tissue DNA Purification Kit. Close Example Yield and Purity with Maxwell® 16 Tissue DNA Purification Kit Plant Tissue Samples 1.Yields determined with PicoGreen ® Reagent (Molecular Probes). 2.Details available at 3.Samples digested with CelluACE XG System (Cat. # FF3800) in the presence of polyvinylpyrrolidone prior to isolation. 4.Samples digested with CelluACE XG System (Cat. # FF3800) prior to isolation. SampleSizeYield 1 Detected by qPCR 2 Arabidopsis thaliana (mustard) 1 leaf139ng + Brassica napus (canola) 5 seeds2.3µg + 8mm punch512ng + Fragaria ananassa (strawberry) 8mm punch193ng 3 + Glycine Max (soybean) 1 seed3.1µg 4 + 8mm punch587ng + Gossypium hirsutum (cotton) 1 seed2.6µg 4 + 8mm punch854ng + SampleSizeYield 1 Detected by qPCR 2 Medicago sativa (alfalfa) 8mm punch1.4µg + Oryza sativa (rice) 1 seed216ng 4 + 5mm punch238ng 4 + Solanum lycopersicum (tomato) 8mm punch734ng + Triticum aestivum (wheat) 5mm slice5.6µg seed1.4µg 4 + Vitis vinigera (grape)8mm punch20ng 3 + Zea mays (corn)8mm punch655ng + Back

20 Rapid Mouse Tail gDNA isolation…for less 5µl of gDNA from 8 x 1cm mouse tail snips Discover how simple your gDNA preps can be Ask for a Demo! Get Maxwell 16 into your lab, so you can Isolate tail gDNA sooner Genotype mice earlier Identify transgenics faster All without Proteinase K! Back


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