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P O P L C ® technology: best separation in one step. Present state and perspective. The New Way to Speed Up H P L C Yuri Kalambet, Yuri Kozmin, Klaus Bischoff,

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Presentation on theme: "P O P L C ® technology: best separation in one step. Present state and perspective. The New Way to Speed Up H P L C Yuri Kalambet, Yuri Kozmin, Klaus Bischoff,"— Presentation transcript:

1 P O P L C ® technology: best separation in one step. Present state and perspective. The New Way to Speed Up H P L C Yuri Kalambet, Yuri Kozmin, Klaus Bischoff, Stefan Lamotte, Rainer Brindle

2 Outline Motivation Method Development in HPLC Phase OPtimized Liquid Chromatography (POPLC ® ) The POPLC ® Optimizer Software Examples Summary

3 RP-HPLC Situation more than 800 RP packings are commercially available today How to find the right packing for my separation?

4 Properties of RP Packings Hydrophobicity Silanophilic Activity Molecular Planarity Recognition (Shape Selectivity) Polar Selectivity Metal Content

5 all modern classical bonded RP packings are looking the same in terms of selectivity the stationary phases are optimized to solve as much applications as possible and are suited for about 80% of all applications today new stationary phases with other selectivities are needed to solve the remaining separation problems Selectivity in RP HPLC

6 Method Development in HPLC Procedure rough choice of the column (C18, polar embedded C18, Phenyl, etc.) Optimization of the mobile phase (pH, solvent strength, if necessary gradient, type of organic solvent, buffer) Optimization of temperature

7 What is POPLC ® ? P hase OP timized L iquid C hromatography ® P ersonal P erformance

8 Scheme Method Development in POPLC ®

9 rough choice of mobile phase (% organic, type, pH) one base measurement on each of n (often 3 to 5) different stationary phases Determination (optimization) of the ideal stationary phase via computer software Procedure

10 = Simple Principal of POPLC ® Retention times are additive !!!

11 1.Mixing of Stationary Phases Possibilities of Realization for POPLC ®

12 2. Combination of Differnent Column Lengths Possibilities of Realization for POPLC ® 1.Mixing of Stationary Phases

13 Dimensions: ID 3.0 mm Lengths: 10, 20, 40, 60, 80 mm Available Column Cartridges

14 Inlet Nut Inlet Adapter Outlet Nut Outlet Adapter Cartridge Holder Segment Cartridge Column Segment

15 Segmented Cartridge Column

16 Colour coded Column cartridges

17 Segmented Cartridge Column One Column Fits All

18 Triazine Pesticides Chemical structures (1) Prometon(2) Simetryn(3) Ametryn(4) Prometryn (5) Terbutryn(6) Simazin(7) Atrazine(8) Propazine

19 List of column segments

20

21 Optimization parameters

22 Best variant

23 Fastest (Optimal) variant

24 Variants list

25 Separation of Triazine Pestizides min min Prediction vs. Measurement Measurement Prediction

26 2. Octogen (HMX) 1. Hexogen (RDX) 3. 1,3,5- TNB 4. 1,3-DNB 14. Tetryl 5. Nitro- benzen 6. 2,4,6-TNT 7. 4-A-2,6-DNT8. 2-A-4,6-DNT 9. 2,6-DNT13. 3-NT10. 2,4-DNT11. 2-NT12. 4-NT Explosives according EPA 8330

27

28 Emulated test run

29 Best resolution variant

30 Measurement Prediction Prof. W. Engewald, Dr. F.-M. Matysik, U. Schuman, Uni Leipzig Separation of Explosives according EPA 8330 Prediction vs. Measurement

31 ProntoSIL C18 SH 2 80 x 3.0 mm ProntoSIL C18 EPS 2 80 x 3.0 mm Mobile Phase: Acetonitrile/20 mM Phosphate Buffer pH 3 30:70 (v/v) Flow rate: 0,5 ml/min Detection: 270 nm Basic Runs on Different Stationary Phases POPLC ® Method Development In Pharmaceutical Industry

32 ProntoSIL Phenyl x 3.0 mm ProntoSIL CN x 3.0 mm Mobile Phase: Acetonitrile/20 mM Phosphate Buffer pH 3 30:70 (v/v) Flow rate: 0,5 ml/min Detection: 270 nm Basic Runs on Different Stationary Phases POPLC ® Method Development In Pharmaceutical Industry

33 Best Separation within 20 Minutes POPLC ® Method Development In Pharmaceutical Industry

34 Best Separation within 20 Minutes POPLC ® Method Development In Pharmaceutical Industry Column:90 mm ProntoSIL C18 SH2 and 20 mm ProntoSIL Phenyl 2 Column Dimension:110 x 3.0 mm Mobile Phase: Acetonitrile/20 mM Phosphate Buffer pH 3 35:65 (v/v) Flow rate: 0,5 ml/min Detection: 270 nm

35 Best Separation within 20 Minutes POPLC ® Method Development In Pharmaceutical Industry The Best Resolution Set POPLC column composition: Segment: C18SH2, length: 10 mm x 9 = 90 mm Segment: Phenyl, length: 10 mm x 2 = 20 mm POPLC column length: 110 mm Void time: 1.23 min Plates per column: 4371 Selectivity: (worst pair: Impurity A - Impurity B) Resolution: (critical pair: 1 - Impurity A) Results Table: Optimized Column Time Component name Impurity A Impurity B Results Table: Predicted Retention Times Results Table: Measured Retention Times Time Component name Impurity A 19.6 Impurity B Results Table: Measured Resolution Resolution: 3.5 (critical pair: 1 - Impurity A)

36 Best Separation within 25 Minutes POPLC ® Method Development In Pharmaceutical Industry

37 Best Separation within 25 Minutes POPLC ® Method Development In Pharmaceutical Industry Column:110 mm ProntoSIL C18 SH2 and 20 mm ProntoSIL Phenyl 2 and 10 mm ProntoSIL CN 2 Column Dimension:140 x 3.0 mm Mobile Phase: Acetonitrile/20 mM Phosphate Buffer pH 3 35:65 (v/v) Flow rate: 0,5 ml/min Detection: 270 nm

38 Best Separation within 25 Minutes POPLC ® Method Development In Pharmaceutical Industry Results Table: Optimized ColumnResults Table: Predicted Retention Times The Best Resolution Set POPLC column composition: Segment: C18SH2, length: 10 mm x 11 = 110 mm Segment: Phenyl, length: 10 mm x 2 = 20 mm Segment: CN, length: 10 mm x 1 = 10 mm POPLC column length: 140 mm Void time: 1.56 min Plates per column: 5429 Selectivity: (worst pair: Impurity A - Impurity B) Resolution: (critical pair: Impurity A - Impurity B) Time Component name Impurity A Impurity B Results Table: Measured Retention Times Time Component name Impurity A Impurity B Results Table: Measured Resolution Resolution: 4.1 (critical pair: 1 - Impurity A)

39 0,00 2,00 4,00 6,00 8,00 10,00 12,00 14,00 16,00 18,00 20,00 Chloridazion HDA DA HA Atrazin Isoproturon Diuron Carbamazepin Diclofenac Chlortoluron Linuron Methabenzthiazuron Metobromuron Monolinuron Metoxuron Metazachlor Hexazinone Simazin Nicosulfuron Thifensulfuron-metyl Metsulfuron-methyl Amidosulfuron Iodosulfuron-methyl Prosulfuron Triasulfuron Triflusulfuron-methyl Bensulfuron-methyl Primisulfuron-methyl Chlorsulfuron Propazin Terbutylazin Metamitron Desethylterbutylazin Retention time in min. C18 Phenyl Polar embedded C18 Retention Behaviour of 33 Compounds in Municipal Waste Water on Different Stationary Phases

40 Prediction Total Ion Count selected MS/MS Transitions Separation of 33 Compounds in LC/MS/MS Optimized POPLC ® Column

41 Separation of 33 Compounds in LC/MS/MS Prediction vs. Reality

42 Isocratic SeparationGradient Separation Separation of 33 Compounds in LC/MS/MS

43 Column: 120 x 3.0 mm ProntoSIL C18 SH : ProntoSIL C18 ace-EPS : ProntoSIL Phenyl 1:3:2 Flow Rate:0.6 ml/min Mobile Phase: 50 % Eluent A: 5 mM NH 4 OAc 50 % Eluent B: MeOH + 5 mM NH4OAc Injection: 10 µl Temperature: 25°C Column: 250 x 4.6 mm Luna C18 (2) Mobile Phase: Eluent A: 5 mM NH 4 OAcGradient: 4 min: 35% B Eluent B: MeOH + 5 mM NH4OAc12 min: 80% B Flow Rate:0.6 ml/min25 min: 80% B Injection: 10 µl Temperature: 25°C Gradient HPLC Separation Isocratic POPLC ® Separation

44 min mAU Scouting Gradient POPLC ® Method Development of a complex unknown mixture Column: POPLink ® column segment ProntoSIL C18 SH2, 40 x 3.0 mm Eluent: A: H3PO4 1 ml/l in H2O; B: ACN; Gradient: 0 – 100% in 10 min. Flow rate: 0.5 ml/min; Detection: 210 nm

45 ProntoSIL C18 SH x 3.0 mm min mAU 2 3,4,B1,B2 B3 6 C ProntoSIL C18 EPS x 3.0 mm min mAU B1 5 6 C B2,B3 Basic Runs on Different Stationary Phases POPLC ® Method Development of a complex unknown mixture Mobile Phase: Acetonitrile/0.1% H 3 PO 4 40:60 (v/v) Flow rate: 0,5 ml/min Detection: 210 nm

46 min mAU 1, 2 3 B1, B2, B3 56 C ProntoSIL Phenyl x 3.0 mm ProntoSIL CN x 3.0 mm min mAU B1, B2, B3 56 C Basic Runs on Different Stationary Phases POPLC ® Method Development of a complex unknown mixture Mobile Phase: Acetonitrile/0.1% H 3 PO 4 40:60 (v/v) Flow rate: 0,5 ml/min Detection: 210 nm

47 min mAU B1 B2, B3 5 6 ProntoSIL C x 3.0 mm Basic Runs on Different Stationary Phases POPLC ® Method Development of a complex unknown mixture Mobile Phase: Acetonitrile/0.1% H 3 PO 4 40:60 (v/v) Flow rate: 0,5 ml/min Detection: 210 nm

48 Prediction of POPLC ® Optimizer Software POPLC ® Method Development of a complex unknown mixture

49 min mAU B1 B2 B3 5 6 Optimized isocratic separation POPLC ® Method Development of a complex unknown mixture Column: 50 mm ProntoSIL C18 SH 2 and 200 mm ProntoSIL C30 Eluent:A: H3PO4 1 ml/l in H2O; B: ACN; 40/60 (v/v) Flow rate: 0.5 ml/min Detection: 210 nm

50 min mAU B1 B2 B3 6 Optimized Gradient Elution POPLC ® Method Development of a complex unknown mixture Column: ProntoSIL C18 SH2 / ProntoSIL C30 50:200, 250 x 3.0 mm Eluent: A: H3PO4 1 ml/l in H2O; B: ACN Gradient: 40% B 50 min.; 40% - 100% B in 85 min. Flow Rate: 0.5 ml/min Detection: 210 nm

51 Advantages of POPLC ® in many cases no gradient elution required easy method development via software easy exchange of column parts (POPLink ® column hardware) every HPLC column selectivity can be simulated This method can be applied in all areas of chromatography from micro to prep LC, in GC, TLC, SPE und Flash Chromatography constant detector background less requirements for HPLC devices no reequilibration required (faster analysis) reusable mobile phase detectors like RI, EC and conductivity are possible

52 Summary Selectivity is the most important tool in HPLC The column is the most important choice The optimization strategy is important POPLC ® offers a simple possibility for future method development

53 POPLC with mobile phase optimization

54 POPLC with gradient ?

55 Other related technologies Peak deconvolution by shape Peak deconvolution by factor analysis Peak identification and matching Retention time prediction with gradient elution


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