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Norton group meeting 11/2010, David Neff Comparison of excitation output Hg vs HgXe vs Xe lamp at objective of TE200 with Optisplit: justification for.

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Presentation on theme: "Norton group meeting 11/2010, David Neff Comparison of excitation output Hg vs HgXe vs Xe lamp at objective of TE200 with Optisplit: justification for."— Presentation transcript:

1 Norton group meeting 11/2010, David Neff Comparison of excitation output Hg vs HgXe vs Xe lamp at objective of TE200 with Optisplit: justification for 2 optics necessary to efficiently couple Xe lamp to o-split scope

2 HgXe lampHg lamp No lamp (ambient, lights off in room but fluor lights in main area leaking in through window) Input optics on HgXe lamp are inferior so do not directly compare absolute intensities. Relative intensities at different wavelength are valid. position 4 GFP ex 470/40 bs 495LP em 525/50 position 2 rhod ex 560/40 bs 595LP | em 630/60 position 1 osplit ex 470/40&550/20 bs 520/40&590/60 em 540LP-green 520/40 -red 595/50 this is the output straight from the lamp no lamp

3 Xe lamp No lamp (ambient, room lights off but light leak in window from main room) Input optics on HgXe and Xe lamp are inferior so do not directly compare absolute intensities. Relative intensities at different wavelength are valid. position 4 GFP ex 470/40 bs 495LP em 525/50 position 2 rhod ex 560/40 bs 595LP | em 630/60 position 1 osplit ex 470/40&550/20 bs 520/40&590/60 em 540LP-green 520/40 -red 595/50 no lamp this is the output straight from the lamp

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5 In the scope: 59004x dual-band exciter 59004bs polychroic mirror In the Optosplit: ET515/30m for GFP ET595/50m for rhodamine T540LPXR

6 biorad mrc1024 leica sp5 20x 100x notice all the contrast in the Leica images almost make them look 'worse'.


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