3 A confirmatory method should . . . Utilize the most accurate (specific) testing method availableHave sensitivity equal to or better than the screening methodBe economically feasibleBe simple enough to standardize across many laboratoriesProduce results that are legally defensible
4 Chromatography Separation of components based on their. . . Solubility in mobile and stationary phasesTerminology:Gas/liquidLiquid/liquidIon exchangePartition
5 Chromatographic separations Mobile PhaseStationary Phase
6 Chromatographic separations BSoluble in stationary phaseLong retention timeSoluble in mobile phaseShort retention time
7 Chromatographic separations The resolution of a chromatographicseparation is defined as:t/mean peak widthBADetector signalTime
9 Chromatographic resolution The resolution, Rs, is a unitless quantity since it is the ratio of two measures of retention (time, volume, or distance).In general, in order to satisfactorily separate equal amounts of compounds A and B, the Rs must be greater than 0.8—baseline separation requires an Rs greater than 1.25.