Presentation on theme: "Characteristics of microbes"— Presentation transcript:
1Characteristics of microbes Major groups of microorganismsViruses Not cells; contain either RNA or DNA in envelope; can only multiply in living cells.Bacteria Unicellular; procaryotic; rigid cell wall; absorb nutrients. Archaebacteria; eubacteriaArchaebacteriaNon-peptidoglycan cell wallsHave unusual metabolic capabilities.Live in extreme environments.Methanogens, halophiles and thermoacidophiles.
2Characteristics of microbes Major groups of microorganisms 2Fungi Either uni- or multicellular; eucaryotic; rigid cell walls; absorb nutrients.Protozoa Single celled; eucaryotic; lack rigid cell wall; ingest food.Algae Either uni- or multicellular; eucaryotic; rigid cell walls; chlorophyll; absorb nutrients.
3Structure/function of eubacteria Size, shape and arrangementDiameterLength Ave.m up to 500 mm wide m long (E. coli )SphericalRodsSpiralcoccus (sing.) cocci (pl.) meaning “berries”. bacillus (sing.) bacillus (pl.) meaning “little staffs”.spirullum (sing.) spirilla (pl.)Pleomorphyvariety of shapes in a species
4Structure/function of eubacteria Size, shape and arrangement 2
5Structure/function of eubacteria Size, shape and arrangement 3
6Structure/function of eubacteria UltrastructureCell wall
7Structure/function of eubacteria Ultrastructure 2Cell wallRigid, strong structure; gives and maintains shape % of dry weight of the cell. Usually essential for cell division.A barrier to some substances. Not homogeneous structures; depending on species, layered with different substances which have different thicknesses.
8Structure/function of eubacteria Ultrastructure 3Peptidoglycan (a.k.a. murein)Shape determining part of the wall. Insoluble, porous polymer of great strength and rigidity.Comprises N-acetylglucosamine, N-acetylmuramic acid and tetrapeptides. Peptidoglycan chains are cross-linked at the tetrapeptide units. Autolysin opens the network for addition of new polymer.
9Structure/function of eubacteria Ultrastructure 3
10Structure/function of eubacteria Ultrastructure 4There are 2 types of cells wall in eubacteriaGram stain differentlyDifferences in the layering and presence of outer membrane (in G -) explains the difference in Gram reaction and pathogencity.
11Structure/function of eubacteria Ultrastructure 5The G(+) cell wallPeptidoglycan content 50%cf. G(-) 10% walls of G(+) bacteria appear thicker.Contains techoic acid (may aid in the transport of cations).
12Structure/function of eubacteria Ultrastructure 5The G(-) cell wallMore complex; has outer membrane covering a thin layer of peptidoglycan.Outer membrane: Contains lipolysaccharides (lipid portion causes toxic effects in infected animals).
13Structure/function of eubacteria Ultrastructure 5Cytoplasmic membraneComposition: Phospholipids % Proteins %
14Structure/function of eubacteria Ultrastructure 6Cytoplasmic membrane 2A site of specific enzyme activity, transport of molecules in and out of the cell, and invaginations participate in cell metabolism and replication.Some processes essential to the cell are located here.A barrier to most water-soluble molecules, and is more selective than the cell wall.Permeases transport small molecules into the cell.Also contains various enzymes for energy production and synthesis of cell wall.Photosynthetic bacteria or those with metabolisms based on gas exchange have elaborate intracellular extensions of the cytoplasmic membrane. This infolding increases the area for light pigments or gas exchange.
15Structure/function of eubacteria UltrastructureCytoplasmic area
16Structure/function of eubacteria Ultrastructure 6Cytoplasmic area80% water with nucleic acids, proteins, carbohydrates, lipids, inorganic ions, many low-molecular weight compounds and particles with various functions.Ribosomes. Free in cytoplasm, not membrane-bound.Cell inclusions (insoluble chemical substances accumulated) e.g. metachromatic granules (a.k.a volutin; a reserve of inorganic phosphate). Lipids e.g. poly--hydroxybutyrate; reserve carbon and energy source. Sulfur: H2S-oxidizing bacteria. Polysaccharides e.g. glycogen and starch; reserve energy sources.
17Structure/function of eubacteria UltrastructureNuclear area
18Structure/function of eubacteria Ultrastructure 6Nuclear areaNo nuclear membrane. Nuclear material found near the center of the cell and is attached to the mesosome-cytoplasmic membrane.Total nuclear material = nucleoid; consist of a single circular chromosome.
19Structure/function of eubacteria UltrastructureFlagella
22Structure/function of eubacteria Ultrastructure 8Pilus(i)Usually longer than fimbriae.Number only one or two.Join cells in preparation for transfer of DNA. Thus also known as sex pili.
23Structure/function of eubacteria Ultrastructure 9GlycocalyxLayer of viscous material (usually polysaccharides) outside the cell wall. Observation: Indian ink stained specimens.When well defined: capsule. disorganized: slime layer.Function: Adhesion; protection against desiccation; prevent attachment by bacteriophages or attack by white blood cells.
24Characteristics of viruses Living organisms?Inert outside a living hostObligatory intracellular parasitesEither: complex aggregation of non-living chemicals or simple living microorganism.Distinctive featuresSingle type of nucleic acid; DNA or RNA.Protein coat surrounds the nucleic acid (NA).Synthetic processes of a host living cell used to multiply.Specialized structures for transfer viral NA to other cells.
26Viral structure Nucleic acid Virion A complete, fully developed viral particle composed of nucleic acid surrounded by a protective coat which also serves as a vehicle of transmission from one host cell to another.Nucleic acidContrasts with pro- and eucaryotic cells where DNA is the primary genetic material.Total NA: From a few thousand to as many as 250,000 nucleotide pairs. E. coli has 4 million nucleotide pairs.NA is single or double stranded; linear or circular.May be in separate segments e.g. influenza virus.
27Viral structure Capsid Protein coat surrounding the nucleic acid. Capsomere: Protein subunits of the capsid. Arrangement is characteristic of each particular virus.Nucleocapsid: Capsid and nucleic acid as a unit.Capsids give virus types their typical shapes.
28Viral structure Envelope Outer membranous layer; lipids, carbohydrates and proteins.Present in many animal and some plant viruses.In animal viruses: Host cell nuclear or plasma membranes contribute envelope lipids and carbohydrates. Envelope proteins are made by the virus.Some have spikes (a.k.a. peplomers); are carbohydrate-protein complexes. Thought to be involved in attachment to hosts. Useful in identification since they differ amongst viruses.
29Viral structure Four general morphological types of virion structure Helical viruses Capsomeres arranged in a helical around the nucleic acid.Polyhedral viruses Capsids are icosahedral in arrangement (20 equilateral triangular faces and 12 corners).Enveloped viruses Roughly spherical but pleomorphic as the envelope is not rigid. Capsid can be helical or polyhedral. Non-enveloped viruses are “naked” viruses.Complex viruses Have capsid symmetry but neither purely helical or polyhedral.
31The eucaryotic cellProtozoa, algae and fungi are eucaryotic.
32The eucaryotic cell Ultrastructure Flagella and cilia Few and long - Flagella Short and few - cilia Motility; movement of substances (cilia) Both: 9 ring pairs and 2 central microtubules.Glycocalyx Surrounds animal cells. Strengthens cells; also means of attachment to other cell.
33The eucaryotic cellUltrastructure 2Cell wall Algae and some fungi: cellulose. Fungi: chitin.Cytoplasmic membrane Like procaryotes: Phospholipid bilayer containing proteins. Contains CHO attached to proteins and sterols not found in procaryotes. Capable of endocytosis.Cytoplasm Resemble that of procaryotes. Has cytoskeleton and exhibits cytoplasmic streaming. Unlike procaryotes, many important enzymes are sequestered in organelles.
34The eucaryotic cellUltrastructure 3Cell wall Algae and some fungi: cellulose. Fungi: chitin.Cytoplasmic membrane Like procaryotes: Phospholipid bilayer containing proteins. Contains CHO attached to proteins and sterols not found in procaryotes. Capable of endocytosis.Cytoplasm Resemble that of procaryotes. Has cytoskeleton and exhibits cytoplasmic streaming. Unlike procaryotes, many important enzymes are sequestered in organelles.
35The eucaryotic cell Organelles Ultrastructure 4OrganellesNucleus Contains DNA in the form of chromosomes. Most characteristic of eucaryotes.
36The eucaryotic cell Organelles Ultrastructure 5OrganellesEndoplasmic reticulum Not present in procaryotes. Provides surface for chemical reactions. Serves as transport network. Stores synthesized molecules.Ribosomes Attached to outer surface of ER but also found free. Larger than procaryotic ribosomes. Site of protein synthesis.
37The eucaryotic cell Organelles Ultrastructure 6OrganellesGolgi complex Consists of cisternae. Secretion; CHO and glycoprotein synthesis.
38The eucaryotic cell Organelles Ultrastructure 7OrganellesMitochondrion(a) Folding of inner membrane provides large surface area for chemical reactions e.g. ATP production (primary site) and cellular respiration. Can multiply on their own by fission.
39The eucaryotic cell Organelles Ultrastructure 8OrganellesLysosomes Formed from golgi complexes. Contain digestive enzymes to breakdown molecules and invasive bacteria.Centrioles Role in cell division and as basal bodies in the formation of flagella and cilia.
40The eucaryotic cell Organelles Ultrastructure 6OrganellesChloroplast Algae/green plants only. Contains DNA, ribosomes, chlorophyll and photosynthetic pigments. Can multiply on their own by fission.