1. Fruit Tree Clean Plant Center WSU - IAREC

2. New personnel: James Susaimuthu supervises virus indexingsupervises virus indexing Sam Hood manages growth facilitiesmanages growth facilities Terri Reddout assists FTCPN managementassists FTCPN management Martin Joseph directs tissue culture programdirects tissue culture program

3. Infrastructure improvements: Greenhouse renovationsGreenhouse renovations CompleteComplete BiosecurityBiosecurity Improved environmental controlImproved environmental control Relocate thermal therapy facilityRelocate thermal therapy facility Near completeNear complete BiosecurityBiosecurity New greenhouse (industry funded)New greenhouse (industry funded) Design completeDesign complete Replaces deteriorated structureReplaces deteriorated structure BiosecurityBiosecurity

4. Asbestos bench tops Gravel floor Deteriorated glazing

6. Replacement quarantine greenhouse designed designed major components purchasedmajor components purchased

7. Upgraded irrigation system

8. Budwood distributions: Domestic releases27,277Domestic releases27,277 International releases1,618International releases1,618 Representing 13 countriesRepresenting 13 countries

9. Selections received: Proprietary55Proprietary55 Foreign28Foreign28 Public17Public17 Indexing only72Indexing only72 Heat therapy20Heat therapy20

10. Thermal therapy 130 selections going through heat therapy130 selections going through heat therapy Includes those received in previous yearsIncludes those received in previous years

11. Current testing cycle in progress: Woody indexing tests20,000Woody indexing tests20,000 Herbaceous indexing (Mar)600Herbaceous indexing (Mar)600 Molecular assays1,500Molecular assays1,500 ELISAs1,500ELISAs1,500

12. Malus:RequiredActual Woody indexing 5 X 4 reps each Herbaceous indexing 44 Field indexing 44 PCR / RT-PCR 2 5 + 4 to confirm ELISA-- Stone fruit: Woody indexing 8 X 4 reps each Herbaceous indexing 44 Field indexing 11 PCR / RT-PCR 3 7 + 3 to confirm ELISA44

13. Current FTCPC research activities: CVA – capillovirus ~ 50% of cherry selections infected~ 50% of cherry selections infected Likely difficult to remove via thermal therapyLikely difficult to remove via thermal therapy Impact unknownImpact unknown Apple green crinkle disease 3 cropping years is required protocol3 cropping years is required protocol Identify pathogen associated with diseaseIdentify pathogen associated with disease Rusty mottle / Twisted leaf Genetically complex family of virusesGenetically complex family of viruses May be able to eliminate some woody indexingMay be able to eliminate some woody indexing