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Data Collection for David Gates Cezanne & Taylor.

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Presentation on theme: "Data Collection for David Gates Cezanne & Taylor."— Presentation transcript:

1 Data Collection for David Gates Cezanne & Taylor

2 B T T T T d1 d2 d5m d3 d4 2 nt clamp, comp to d5 Davids PNAS Catalytic Rxn (A+B->A+C) DG1s DG2-AC d1 T T T d3 d4 A Fuel-s Rep. CA Courtesy of Matt Olson, 2010

3 A+B->A+C Courtesy of Matt Olson, 2010

4 X #Contents 1Ladder 2DG2 3DG2 + Fuel S 4DG2 + DG1 5DG1 6DG1 + B 7DG1 + A + B 8Rep. Gate 9Rep. Gate + DG2

5 A1 B1 Backward BOut B Helper BC B1 C2 Gate B (GB) Translator Gate BC (TGBC) Reporter Gate C (RGC) Gates for A1 + B1 -> B1 + C2

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9 #Contents 1Ladder 2TGBC 3TGBC + Helper BC 4GB 5GB + A1 6GB + B1 7GB + A1 + B1 8Rep. Gate C 9TGBC + Rep. Gate C

10 A1 B1 Backward BOut B Helper B B1 B2 Gate B (GB) Translator Gate B (TGB) Reporter Gate B (RGB) Gates for A1 + B1 -> B1 + B2

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13 #Contents 1Ladder 2TGB 3TGB + Helper B 4TGB + GB 5TGB + Rep. Gate B 6Rep. Gate B 7Rep Gate B + B1 8Rep. Gate B + B2 9Rep. bot B + B2 10Rep. bot C irr + C2 11Rep. bot B

14 A_1: CACAATACTATCATC AATCCCT CACTCTTCAAACCTC TAACCCT B_1: CATTCAAATCTCACC TTTCCCA CTAACAACTCTCTAC TAACCCA B_2: CCATTCATTCTCTCC TTTCCCA CTAACAACTCTCTAC TAACCCA C_2: CCTTCTTACACCTTC ATACCCT CTCATTCCTCACAAC ATTCCCA Backward B: CTAACAACTCTCTAC TAACCCA AATCCCT Out B: CACTCTTCAAACCTC TAACCCT CATTCAAATCTCACC TTT Gate_bot B: TG AGGGTTA GAGGTTTGAAGAGTG AGGGATT TGGGTTA GTAGAGAGTTGTTAG TGGGAAA Helper B: CCCA CT CCATTCATTCTCTCC TTTCCCA Trans_bot B: AG TGGGAAA GGAGAGAATGAATGG AG TGGGAAA GGTGAGATTTGAATG AGGGTTA Helper BC: CCCA CT CCTTCTTACACCTTC ATACCCT Trans_bot BC: AG AGGGTAT GAAGGTGTAAGAAGG AG TGGGAAA GGTGAGATTTGAATG AGGGTTA Rep_top B: CTAACAACTCTCTAC TAACCCA CCCAA /3IAbRQSp/ Rep_bot B: /56-ROXN/ TTGGG TGGGTTA GTAGAGAGTTGTTAG TGGGAAA Rep_top C irr: CTCATTCCTCACAAC ATTCCCA /3IAbRQSp/ Rep_bot C irr: /5TYE563/ TGGGAAT GTTGTGAGGAATGAG AGGGTAT


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