1. EPS15L1 is predominantly expressed in neurons and surviving Eps15L1-KO mice have development and neural deficits.
EPS15L1 is predominantly expressed in neurons and surviving Eps15L1-KO mice have development and neural deficits. (A) Western blotting of the heart (Ht), ovary (Ov), testis (Te), colon (Co), lung (Lu), liver (Li), spleen (Sp), brain (Br), cerebellum (Ce), and fibroblasts (MEFs); 10 μg of each tissue lysate were loaded and probed for EPS15. Specific bands are indicated by arrows. Various amounts of GST-fusion protein for EPS15 or EPS15L1 were also loaded to allow for a rough estimate of the actual amount of the protein in the cellular lysates. (B) Western blotting as in (A), probed for EPS15L1. Specific bands are indicated by arrows. (C) Immunofluorescence for EPS15 and EPS15L1 expression in the hippocampus of adult mouse brain. Sections were stained for EPS15 or EPS15L1 as indicated (green), synaptophysin (red) and counterstained with DAPI (blue). Bar, 10 μm. (D) Western blotting of adult mouse brain fractions with the indicated antibodies. Equal amounts of homogenate (H), synaptosomal fraction (Syn), and postsynaptic density (PSD) were loaded. Note that, as expected, PSD95 (a post-synaptic marker) was enriched in the PSD, whereas synaptophysin was enriched in the synaptosomal fraction. (E) WT and Eps15L1-KO mice at 4 wk of age. (F) Bodyweight of WT/HET and Eps15L1-KO mice at 4 wk of age. Data from WT and heterozygous animals were pooled as no statistical difference between the two genotypes was detected. The number of mice analyzed is shown in parentheses. (G) Results of a reduced SHIRPA test performed on 4-wk-old WT and Eps15L1-KO mice. **P < 0.01 versus WT; ***P < versus WT
Cinzia Milesi et al. LSA 2019;2:e
© 2019 Milesi et al.