Presentation is loading. Please wait.

Presentation is loading. Please wait.

DISIAPKAN OLEH PROF DR.PRATIWI TS, DRH, MS AI-TEKREP PET 2012-Prof Pratiwi 1 TEKNOLOGI REPRODUKSI dan INSEMINASI BUATAN PET ANIMAL.

Similar presentations


Presentation on theme: "DISIAPKAN OLEH PROF DR.PRATIWI TS, DRH, MS AI-TEKREP PET 2012-Prof Pratiwi 1 TEKNOLOGI REPRODUKSI dan INSEMINASI BUATAN PET ANIMAL."— Presentation transcript:

1 DISIAPKAN OLEH PROF DR.PRATIWI TS, DRH, MS AI-TEKREP PET 2012-Prof Pratiwi 1 TEKNOLOGI REPRODUKSI dan INSEMINASI BUATAN PET ANIMAL

2 SWEETEST PUPPIES AI-TEKREP PET 2012-Prof Pratiwi 2

3 Stages of the Ovarian Cycle AI-TEKREP PET 2012-Prof Pratiwi 3 Anestrus Proestrus Estrus Diestrus

4 Estrous Cycle Proestrus (9 days) Vulva swollen Bloody discharge Attracted to male but does not mate Estrus (9 days, ovulation day 2) Accepts male Straw-colored discharge Metestrus (90 days) False pregnancy Anestrus (75 days) Sexual inactivity 4 AI-TEKREP PET 2012-Prof Pratiwi

5 The Bitch Puberty - 6 mo. Monoestrus One estrus during breeding season Estrous Cycle Proestrus Estrus Metestrus Anestrus Heat 5 AI-TEKREP PET 2012-Prof Pratiwi

6 Estrous Cycle Proestrus (9 days) Vulva swollen Bloody discharge Attracted to male but does not mate Estrus (9 days, ovulation day 2) Accepts male Straw-colored discharge Metestrus (90 days) False pregnancy Anestrus (75 days) Sexual inactivity 6 AI-TEKREP PET 2012-Prof Pratiwi

7 Hormonal Changes Proestrus Estrus Anestrus FSH E2E2 E2E2 LH Progesterone Days Ovulation 7 AI-TEKREP PET 2012-Prof Pratiwi

8 Day of Metestrus 6080 Progesterone E2E2 No Uterine Luteolytic Mechanism Prolactin Heat 20 day Metestrus I Metestrus II 70 days 8 AI-TEKREP PET 2012-Prof Pratiwi

9 Estrous Cycle AI-TEKREP PET 2012-Prof Pratiwi 9 Pro-estrus » days » Attracted to males » Rubs head and neck on objects » Vocalization, posturing and rolling Estrus » Accepts male » days if male present, 10 days if no male » Ovulation 27 hours after mating (induced) » Affectionate to aggressive towards owners

10 Estrous Cycle AI-TEKREP PET 2012-Prof Pratiwi 10 Proestrus if queen did not ovulate » days Diestrus after ovulation » psuedopregnancy - 40 days » pregnancy - 60 days Anestrus months

11 AI-TEKREP PET 2012-Prof Pratiwi 11

12 Changes During Estrus of Bitch Proestrus Estrus Aggressive towards Male Passive Provides sexual posturing to male for copulation Bloody discharge from vulva Copulation Vulva Swollen and Enlarged Indicates Day of Peak in LH AI-TEKREP PET 2012-Prof Pratiwi 12

13 AI-TEKREP PET 2012-Prof Pratiwi 13

14 AI-TEKREP PET 2012-Prof Pratiwi 14

15 Fantasizing… AI-TEKREP PET 2012-Prof Pratiwi 15 Haloo... My dear

16 Anestrus AI-TEKREP PET 2012-Prof Pratiwi 16 Uterine involution post parturition or when P4 levels are at baseline Time between heat cycles Hormones – Estrogen & P4 are low Duration - Avg of 4.5 months Vaginal Cytology – Parabasal and intermediate vaginal epithelial cells, +/- WBCs & bacteria, no RBCs

17 Vaginal Cytology Correct collection of a vaginal cytology preparation is critical to its diagnostic value! AI-TEKREP PET 2012-Prof Pratiwi 17

18 Proestrus AI-TEKREP PET 2012-Prof Pratiwi 18 Time when bleeding begins and the bitch refuses to be bred, males are attracted to her, swelling of vulva Duration – Avg 9 days (range: 6-11 days) Hormones – Estrogen is increasing; P4 baseline Vaginal Cytology – RBCs, Decreasing WBCs, & parabasal cells, bacteria present, increasing intermediate, superficial intermediate, & few cornified cells

19 Vaginal Cytology - Proestrus AI-TEKREP PET 2012-Prof Pratiwi 19

20 Estrus AI-TEKREP PET 2012-Prof Pratiwi 20 Time when bitch will allow the male to breed her until she will no longer stand Standing Heat Hormones – Estrogen fall & P4 rises; LH surge occurs Vaginal Cytology – RBCs decreasing or absent; no WBCs; Bacteria present; >80-90% Cornified cells

21 Vaginal Cytology - Estrus AI-TEKREP PET 2012-Prof Pratiwi 21

22 AI-TEKREP PET 2012-Prof Pratiwi 22

23 INSEMINATION OF THE BITCH AI-TEKREP PET 2012-Prof Pratiwi 23 Bitches ovulate around day 10 after they enter proestrus (discharge) or about days of estrus. Ovulation can be detected by: – LH assay (peak LH value + one day) – Progesterone assay (>5 ng/ml) – Cytology of vaginal smear (>50% cornified cells)

24 AI-TEKREP PET 2012-Prof Pratiwi 24 Remember oocytes in the canine are ovulated as 1° oocytes and must mature in the oviduct to a 2° oocyte before fertilization Fresh or cooled semen, inseminate 2 days after ovulation detected and again hr latter. Frozen semen, inseminate on day after ovulation Uterine insemination better than cervical

25 25 AI-TEKREP PET 2012-Prof Pratiwi

26 Timing of Insemination AI-TEKREP PET 2012-Prof Pratiwi 26 Natural breedings or Fresh Collection AI 3 breedings between d1 and d6 Fresh chilled-extended semen 1 breeding on d5 2 breedings on d4 & d6 3 breedings on d2, d4 & d6 Frozen semen 1 breeding surgically/transcervically on d6 2 breedings transcervically/surgically d4 & d6

27 AI-TEKREP PET 2012-Prof Pratiwi 27

28 Estrous Cycle FELIN Pro-estrus days Attracted to males Rubs head and neck on objects Vocalization, posturing and rolling Estrus Accepts male days if male present, 10 days if no male Ovulation 27 hours after mating (induced) Affectionate to aggressive towards owners 28 AI-TEKREP PET 2012-Prof Pratiwi

29 Mating Queens call or vocalize (low moaning sound) Owners may think a sign of illness During mating Tom bites neck female With erection penis faces forward Only lasts 30 seconds to 5 minutes As male dismounts female gives a loud copulatory call and Tom retreats Mating occurs times until queen declines May occur for up to 4 days 29 AI-TEKREP PET 2012-Prof Pratiwi

30 Mating Occurs during estrus Length of courtship by male is variable Licks vulva Bitch stands with tail to one side Mounting Male mounts without erection and penetrates Os penis In vagina, male bulbus glandis engorges, thrusting Male dismounts and turns around but penis remains in vagina the tie Ejaculation Tie last 5 min to 1 hour 30 AI-TEKREP PET 2012-Prof Pratiwi

31 Mating (cont.) Mate 2 times Days 11 and 13 after start of proestrus Sperm lasts up to 7 days in female Infertility Often from mating not at appropriate time Hormonal detection of ovulation LH increase associated with progesterone >0.5 ng/ml Ovulation progesterone 2-5 ng/ml 31 AI-TEKREP PET 2012-Prof Pratiwi


Download ppt "DISIAPKAN OLEH PROF DR.PRATIWI TS, DRH, MS AI-TEKREP PET 2012-Prof Pratiwi 1 TEKNOLOGI REPRODUKSI dan INSEMINASI BUATAN PET ANIMAL."

Similar presentations


Ads by Google