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Lavanyas Vision Our vision is to see a healthier world with the help of ancient Ayurvedic achievements and new age scientific technology. We aspire.

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Presentation on theme: "Lavanyas Vision Our vision is to see a healthier world with the help of ancient Ayurvedic achievements and new age scientific technology. We aspire."— Presentation transcript:

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3 Lavanyas Vision Our vision is to see a healthier world with the help of ancient Ayurvedic achievements and new age scientific technology. We aspire to blend the science of Ayurveda with modern medical research methodology to provide natural solution to human ailments.

4 Modern Science & Ayurveda Modern Latest Technologies to diagnose at Physical Level. Modern Science treatment has Merit of Surgery, Antibiotics, Emergency and Symptomatic treatments on physical level. But at the same time it has its own limitations. Ayurveda But in Ayurveda the Diagnosis is made on Physical (Constitutional), Mental (Emotional) and Soul Level. Ayurveda has 2000 years old documentation but due to the lack of scientific evaluation

5 Modern Science & Ayurveda Modern It is far behind the modern system of medicine. Since past few years we are working on scientific parameters.

6 Quality analysis & standardization A. For Ayurvedic Bhasma classical Bhasma Pariksha 1.Rekha purna (Bhasma particles enters in to furrows of the finger. This indicates the fineness of the particles.) 2.Varitar (floats over the stable water surface. This test indicates the lightness of Bhasma) 3. Unam (Putting a grain over the floating particle) 4. Nirdhum (does not yield fumes over fire) 5. Nishchandra (absence of metallic lustre)

7 Quality analysis & standardization B. Elemental assay for Bhasma by AAS method at Biotech Park Lab. C. Raw Material & finished product are analyzed and compared with standard (API)TLC, Total Ash Acid Insoluble Ash, Alcohol and water soluble extractive, loss on drying at 105 C D. Isolated components for standardization of different samples (eg. Humic acid & Fluvic acid for Shilajeet by HPLC) wherever applicable.

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9 OUR AIM Researches on :- 1.Cancer: Second commonest cause of death. 2.AIDS: Dreaded disease of the decade. 3.Diseases Refractory to modern treatment e.g. Hepatitis, RA, Osteoporosis etc. 4.Evaluation of Ayurvedic drugs using Modern Parameters for their efficacy and safety. 5.Evaluation of Ayurvedic Diagnostic procedures and to correlate them with modern parameters. 6.Modification of the dosage form by Nano Technology. 7.In-vitro Studies: Cell line, Cell cycle (Flow Cytometry) and current Tissue Culture technique. 8.Quality Analysis with Standard and to develop current method of Standardization of drugs which are not mentioned in API.

10 In-vitro cell line study

11 In-vitro Cyto Chemical & Flow-cytometry – Facility provided by CDRI, Lucknow Cancer cell lines used were breast cancer (MCF7), cervical cancer (HELA), colon cancer (COLO-205) and prostate cancer (DU-145) LAS02 inhibits proliferation by inducing retention of cells by 79.28% at 400 µg/ml of dose in G0/G1 phase along with apoptosis induced 43.51% at same dose significantly at in-vitro level.

12 Flow- cytometric responses with 400 µg/ml dose of LAS02 Apoptosis Diploid G1 Diploid G2 Diploid S 400 µg/ml % of Apoptosis & Retention of Cell

13 Maximum Response in G0/G1 Phase of the Cell Cycle as seen by Flow-cytometry (B) Cell cycle result for 48 hrs in Prostate Cancer Cell Line (A) Cell cycle result for 24 hrs. in Colon Cancer Cell Line

14 MG63- osteosarcoma cell line

15 Expression of Proteins

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18 Expression of Proteins p53. Effect on Expression of TSG,POG & anti ROS Parameters. p53 Bcl2 Caspase Survivin NF Kappa B LAS02 a Cancero-static compound enhanced the expression of p 53 in different type of malignancies. p 53 values significantly (4.76U/ml 35.94U/ml).

19 Effect on Hormones profiles

20 Chakras (Energy stores) Kundalini jagran modifies hormonal profile by LAS Antistress – A/ACTH/CARTICOIDS 2.Pineal Gland (3 rd eye):- Melatonin, Serotonin. 3.Other releasing hormones and targeted hormones is under study with ELISA by using Kits. 4.Effect of selected Ayurvedic drugs on hormonal profile such as A. LAS-01 B. Energy management

21 In Vitro USA study

22 Isolated CANCER Cell lines or naturally Cultured cancer tissues for screening anti cancer drug (USA).

23 Results & Conclusion Live cell imagining and proliferation data demonstrate: Most effective and time-depended anti- tumor activity was observed at 25 g/ml treatment dose of Las01 agent with MDA0132 and HT-29 Microtumors.

24 Isolation of DNA & Sequencing

25 Expression analysis of several proteins expressed during Cancer The effect and change on the genetic and expression level has to be analyzed by studying the protein expressed by the genes related to cancer and modulation or mutation that bring these effects and changes can be analyzed so as to know the exact drug activity and pattern of action on various cancerous cells. The studies involve RNA isolation and further the expression analysis of the same using reverse transcription PCR (Polymerase Chain Reaction) and primers of different proteins expressed during cancer. This will also clearly demonstrate the effect of drug and changes made by it at the DNA level for different doses.

26 Toxicity Study Herbo-mineral therapeutics are one of the most promising area of treating disease like cancer. The branch of Ayurveda which deals with medicinal properties of herbo- metallic and herbo- mineral preparations is known as RASASHASTRA and the drugs which are used in the branch known as RASA AUSHADHIS. They were more popular during the periods of the LORD BUDDHA due to faster relief, lesser and agreeable dose and mysterious efficacy as compare to only herbal drugs. So herbo-mineral drugs are considered more useful and effective as medicines for Cancer in many ways, such as prophylactic, palliative, curative and supportive. Rasayana therapy and its role in cancer management are being screened in almost all leading Ayurved research institutes. Polyherbal formulations based on Rasayana therapy described in Charak Shamhita showed remarkable improvement in quality of life of various Cancer patients who were receiving chemotherapy, radiation and to overcome the side effects of chemotherapy and radiation such as hair loss, weight loss, stomatitis and zerostomia.

27 Toxicity Study A dose response acute toxicity study with LAS01 showed that there was no mortality in mice which was observed for 72 hours. LAS01 for 90 days (Chronic Toxicity) did not show any concurrent on drug control in term of Haemoglobin percentage and TLC etc. based on the test parameters used. It appeared that the LAS01 did not significantly affect the Hepatic and Renal functions. It was observed that there are no symptoms of toxicity and it does not produce any degenerative changes of tissue damage to significant extent in the organ studied. At the normal therapeutically equivalent dose and fivefold higher dose; none of the biochemical and GBP parameters showed any significant toxic changes. Hence the drug may be labeled as Non Toxic.

28 DNA Study Expression analysis of several proteins expressed during Cancer The anti-cancer drug of Lavanya Ayurveda Pvt. Ltd. LAS01 and LAS02 have been found effective through various experiments like MTT and Flowcytometery analysis, but the effect and change on the genetic and expression level has to be analyzed by studying the protein expressed by the genes related to cancer and modulation or mutation that bring these effects and changes can be analyzed so as to know the exact drug activity and pattern of action on various cancerous cells. The studies involve RNA isolation and further the expression analysis of the same using reverse transcription PCR (Polymerase Chain Reaction) and primers of different proteins expressed during cancer. The expected results would demonstrate the relation between the protein regulation and the disease progression. This will also clearly demonstrate the effect of drug and changes made by it at the DNA level for different doses.

29 Effect on CD 4 count and Viral Load AIDS is the deadliest disease of the decade, for which unfortunately even after several advancement in biomedical sciences, no curative treatment is available as no drug is capable of separating and recovering the motif between retro- viral DNA of the host. A study has been undertaken to evaluate the role of our Anti-AIDS Herbo-Mineral Compound (LAA-1) for assessing antiviral activity includes CD3, CD4, CD8, CD4/ CD8 ratio and viral load parameters. The result of the study indicate that a significant rise in CD4 counts (p<0.05) and a significant decrease in viral load (p<0.05). The Ayurvedic Herbo-mineral preparation LAA01 under test is not only a viridicidal (drug inhibiting the HIV replication) as is evident from decrease in HIV load, but the drug also enhanced immunity as is evident from increases in CD4 and CD8 counts and their respective percentage, which maintained the quality of life (QOL) of the patients.

30 Cancer Cell Line bank Lavanya is developing a Cancer Cell Line Bank to study The Anti-cancerous activity of our Herbo- minerals used in various types of malignancies To ensure its efficacy, To ensure its safety To ensure its non toxic property Cell line study helps to ensure the efficacy of Lavanyas treatment on other incurable diseases

31 Tissue Culture Lab Lavanya Ayurvedic Pvt. Ltd. has established a Tissue Culture Lab and two projects are submitted to state DST and Dept. of Biotechnology (GOI) for approval. Production of Katuki project has already been prepared and in process of submission to Dept. of Medicinal Board for approval. Musali project is in process and as per the protocol media has been prepared.

32 Our Major Achievement It was found while treating thousands Cancer patients that metastasis of the disease even in 4th stage is completely checked up without any recurrence. If the malignant mass or growth is not necrosed, it becomes dissolved and excreted out by various microcirculatory channels within 4 to 8 month (varying to severity and involvement of deeper Dhatu as bone) after taking Lavanya Ayurvedic Therapy. External malignant wounds are completely treatable by Lavanya Ayurvedic Therapy. But in case of internal wounds which become necrosed need surgical removal, after that they could be healed by Ayurvedic Therapy even in 4th stage of disease. Radiation and Chemotherapy is not required at all.

33 Our Major Achievement There are false believes about Bhasma prepared by heavy metals like Hg, As, Pb, Cu etc are very toxic to body. Lavanya has proven that if the Bhasma are prepared according to classical Ayurvedic texts and dose is proper administered. While treating thousands patients with Ayurvedic rasa rasayana therapy there were no adverse effects of after effects were found in all patients and it was regular monitored clinically and hematologically (Liver & Renal function test). Astha Sanskarit Parad (Processed mercury) as described in Ayurvedic texts, most effective rejuvenator is first time prepared by only in Lavanya

34 Drug Standardization Drug Standardization on Modern Parameter, Acient parameter quality The issue of Drug Standardization has ever been so important in respect of Drug Industry either on small level or on large scale industry. At Lavanya Ayurveda, where a large number of Ayurvedic formulations of various herbal and herbo- mineral sources are prepared for in house day to day use for patients either attending the out patients department or admitted for their treatment at in patients level. The quality control of the formulations has a vital role. Normally the standards as laid down in Indian Pharmacopoeia (I.P.) and Ayurvedic Pharmacopoeia of India (A.P.I.) are taken into account for ascertaining the standard or herbal or herbo- mineral substances. Lavanya has always been engaged in formulating and preparing all of its formulations in strict compliance of classical methods, as well as IP and API standards, besides GMP and FDA rules and norms.

35 Drug Standardization To comply with the standards or A.P.I. and I.P., the drug at first is graded for a better selection of raw material. Thereafter it is washed with running pure water to remove the dust and then dried well. There after it is grinded in Pharmacy department. Before it is passed on to the manufacturing dept., the raw drug is subjected for its physico- chemical analysis at Quality Control and Quality Assurance Division of Lavanya Ayurveda which is a well equipped Laboratory. The drug confirming the standards of A.P.I. and I.P. are used in the manufacturing of various formulations either herbal or herbo- mineral.

36 Drug Standardization Different tests on the basis of Ayurvedic Pharmacopeia of India (API) are performed at Lavanya: (A) Description i- Macroscopic ii- Microscopic (B) Identity, Purity and Strength i- Foreign matter ii- Total Ash iii- Alcohol soluble extractive iv- Water soluble extractive v- Acid insoluble Ash vi- Loss on drying vii- pH (C) Assay/ TLC Constituents (D) Phytochemical analysis (E) Microbial limit test (F) Classical bhasma testing Rekhapurnatwa, Varitaratwa etc. are performed for metallic Bhasma.

37 Quality control Department Quality Control (Q.C.) of a drug is as necessary for use in the treatment as a good food for proper physical and mental development. Lavanya keeps its eye on every point so that the raw drugs and finished products showed to be full of established quality parameters. For this purpose the source of procurement of raw material is very much important. Sorted only patients raw-material is procured which must be having sufficient chemical constituents as well as away from any contamination and decomposition. The stage between the raw drug and the final or finished product is method of preparation. Lavanya follows strict adherence to classical methods and no deviation at any level is permitted in preparing or purifying raw drugs. G.M.P. and F.D.A. rules help a lot in this direction which are invariably adopted and followed by the manufacturing section with the expert assistance of Drug Standardization and Quality control division.

38 Biosynthesis of Metal Based Plant Nanoparticles and its Anticancer Activity

39 INTRODUCTION Nanotechnology research is interdisciplanary with physic, chemistry, biology, material science and medicine. Plant are the rich source of various biocompounds in combination such as meniral, vitamin, alkylating agent, prophylineactic agent, protiens, fibres, flavanoids, phenolic compounds and so on. Although an array physical and chemical method have been used to synthesized plant based silver nanoparticles.

40 OBJECTIVE The objective of the present work is, formation of medicinal plants based Silver Nanoparticles and their Anticancer activity. Firstly we have prepared Nanoparticle of Curcumin longa with the help of silver nitrate. MATERIAL AND METHOD The dried powdered of C. longa was used for extraction process. 10gm of dried powder was continuously extracted with ethanol. The extract was stored at -4c for further analysis. SYNTHESIS OF SILVER NANOPARTICLES A 10ml of Curcumin longa extract was added into 90ml of aqueous solution of 1mM silver nitrate (AgNo 3 ) for reduction of silver nitrate into Ag+ ion and keep at room temperature for upto 5 hours.After 20 minutes the colour of the solution changed into yellowish black indicating the formation of silver Nanoparticles.The bio reduced silver Nanoparticles solution was filtered and filtrate was measured using UV-Visble absorbance.

41 RESULT UV-visible spectra analysis The progress of the reaction between metal ions and extract were monitored by UV-Visible spectra of silver Nanoparticles in aqueous solution after diluting a small aliquot of 500µl of the sample with 1ml deionised water in different wavelengths i.e.,335,340,380, and 420nm and in different reaction times i.e.1,2,3,4 and 5 hours at 340nm. The scanning graph obtained from UV-Visible spectrometry gives the range of particle at 340nm and its absorbance is possibly the formation of curcuma silver Nanoparticles

42 Graph from UV-VIS Spectroscopy

43 CONCLUSION Plant based Nanoparticles are new and exciting field of interdisciplinary Science, which make us to use plant in effective manner FUTURE WORK To check the anticancer activity of C. longa Nanoparticles with the help of exploring the mechanism of its activity Formation of other medicinal plant based Nanoparticles.

44 A novel drug delivery: Nanomedicine Nanoparticles are promising tools for the advancement of drug delivery. However, the bio distribution of these nanoparticles is still imperfect due to the complex host's reactions to nano- and microsized materials and the difficulty in targeting specific organs in the body. At Lavanya Ayurveda R & D center researches are going on to optimize and better understand the potential and limitations of nanoparticulate systems

45 Expertise in dosage forms Devlop standred of dosage Ayuvedic Medicine, Herbs & not at develop. At Lavanya Center different type of suitable dosage form are prepared, which are tested for its identity, purity and strength before human use. Arka, Capsule, Cream, Ghana Satwa, Lotion, Eye drop are frequently prepared

46 In Vitro Cancer Cell Line Study Effect of LAS01 an Ayurvedic Herbo-mineral compound on anti- proliferative activity and induction of Apoptosis in Human Breast Cancer MCF-7 Cell line. For apoptotic induction study we used two apoptotic parameters F.actin ring positive cells and Vitronectin receptor positive cells using immune- cytochemistry. Cells were cultured in standard situation using DMEM medium. Cells were treated LAS01 along with a control group treated with DMSO. After 24 and 48 hours, of the cells were treated with F. actin and Vitronectin primary antibodies following incubation of secondary antibodies. F. actin ring positive cells and Vitronectin receptor positive cells were observed in Lieca Microscope under florescent die using cell counter with cell morphometric analysis provided with Microscope.

47 In Vitro Cancer Cell Line Study As study the compound shows induction of Apoptosis in carcinoma cell lines. To find out better apoptotic result, experiments were repeated with higher dose 100, 200 and 300μg/ml of LAS01 in ethanol. The result shows 9.26%, 13.31% and 17.68% in F. actin positive cells and 8.62%, % and % in Vitronectin receptor positive cells higher as compared to control group. All the doses did not show Necrosis in cells. Growth of control group and Proliferation in treated cell were noted. It was found that in comparison to control group 48.6% less proliferation was there in LAS01 treated group at 300μg/ml dose MTT assay was also performed. The data suggest maximum inhibition about 48.6% in the treatment of 300 μg/ml as compared to control group. The treatment of 10, 20 and 50 μg/ml dose inhibit 16%, 21%, 33% proliferation respectively in control Group

48 Cell Cycle study Our herbo- mineral drug LAS02 arrests the cancerous cells in cell cycle at G0/G1 phase, and prevented the entry of pre-cancerous stem cells from G0/G1 phase into G2, the subsequent proliferative stage and inhibits cancer cells from completing the cell cycle. In cell cycle analysis for COLO-205 and DU-145 cell lines with potent anti- cancerous compound LAS02 at doses of µg/ml in MTT assay showed inhibition of proliferation and decreased the viability of the cancers cells significantly. The percentage retention of the cells in cell cycle for COLO-205 at G0/ G1 phase was 73.07% at dose 300µg/ml of LAS02, as compared to 52.16% in the control after 24hrs. In cell cycle analysis for DU-145, treated with LAS02, cells that retained at G0/G1 phase were 79.28%, at a dose of 400µg/ml after 48hrs, as compared to control of 62.41%.The apoptosis observed, at 400µg/ml drug concentration was 43.51%. The result of study shows that LAS02 acts as a potent anti-cancerous compound by inhibiting proliferation as well as by inducing retention of cells in G0/G1 phase along with apoptosis significantly at in-vitro level.

49 Publications 1. Toxicity of A Novel Herbo- mineral Preparation LAS01 On Human Cancer Cell Lines And Its Safety Profile In Humans and Animals Summary: In present study hematological and biochemical parameters found safe at different dose level in animal. In human volunteer at therapeutic dose level it is found safe at complete course of treatment. LAS01 is effective in human cancer cell line of cervical (Hela) and Breast Cancer Cell line (Adenocarcinoma MCF 07).

50 Publications 2. LAS01 Novel Herbo- mineral Ayurvedic Drug inhibits proliferation and induces the apoptosis in MCF-7 breast and Hela cervical cancer cell lines Summary: Results show inhibition and anti proliferative activity of compound in a dose dependent manner and maximum anti-proliferative activity about 77% in MCF-7 and 78% in Hela cells were observed in a dose of 500µg/ml. In case of anti apoptotic activity the results indicate maximum apoptotic inducing activity of 56.8% in 500 µg/ml dose in MCF-7 & 58.2 % in Hela VNR positive cells and 57.6 % in MCF-7 & 59.3 % in Hela F- actin cells respectively which was found to be significantly higher as compared to control group. The findings show a potent anti- proliferative and apoptosis inducing activity of compound LAS-01. The effects on cell proliferation exhibit a potent anti-proliferative activity of LAS01 and a comparable apoptotic inducing activity of various doses of LAS01 which suggests a strong in vitro anti- cancerous activity of this promising compound.

51 Publications 3. Inhibition of Proliferation The experiment to quantify the inhibition of proliferation of different cancer cells by the drug LAS02 has shown different and significant results on different cancer cell lines. In the colon and prostate cancer cells (COLO-205 and DU-145) the drug inhibited the cancer cell to 52.95% and 51.90% respectively in 24 hour duration at 600µg/ml dosage as published in 5th World Ayurvedic Congress at Bhopal. The drug LAS02 has shown 65.04% inhibition on lung cancer cells (A-549) in the duration of 24 hours at a dosage of 800 µg/ml. Another experiment designed to observe the effect of drug on Mouth cancer cells on long term dosage showed 70.43% inhibition in 2 days, 78.51% inhibition in 4 days, and 83.21% inhibition in 8 days duration at the dosage 600 µg/ml.

52 Publications Cell Cycle Analysis The cell cycle analysis was done by flowcytometry analysis showing G1/G0 phase arrest on the treatment of LAS02 on different cell lines. The colon cancer has shown cell retention of 73.07% at 300µg/ml, as compared to 52.16% in the control after 24hrs. The prostate cancer cells have shown 79.28% retention of cells, at a dose of 400µg/ml after 48hrs, as compared to control of 62.41%. The lung cancer cells (A-549) have shown 74.55% retention of cells at 400 µg/ml.

53 Publications Apoptosis The drug LAS02 has induced the activity of apoptosis, which has been seen in different cancer cells by the flowcytometry experiment on these cells after treatment. The prostate cancer cells (DU-145) on treatment of LAS02 at a dosage of 400µg/ml for 48hrs had shown 43.51% of apoptosis. The lung cancer cells (A-549) on treatment of LAS02 at a dosage of 400µg/ml for 48hrs had shown 12.86% of apoptosis.

54 Publications Fluorescent Staining The cells of mouth cancer after treatment with LAS02, were stained and were observed under fluorescent microscope to analyze the effect of the drug. Control After LAS02 dosages 50µg/ml for 48 hrs After LAS02 dosages 100µg/ml for 48 hrs After LAS02 dosages 400µg/ml for 48 hrs

55 S.No. Types of CATotal Screened Cases 1.Ca. Mouth Blood Cancer75 3.Ca. Esophagus64 4.Ca. Bone70 5.Ca. Brain45 6.Ca. Stomach62 7.Ca. Liver85 8.Ca. Lung66 9.Ca. Lymphoma10 10.Ca. Breast Ca. Rectum Ca. Prostate27 13.Ca. Pancreas51 14.Ca. Kidney27 15.Ca. Testis19 16.Ca. Ovary/Uterus/ Cervix 121 Total2367 We have data of more than patients of cancer during the 15 years of our services. Cases seen related to cancer of different organs from 1st feb., 2010 to 30th june 2012

56 Some Report of Cases

57 Ca. Gall Bladder S. N o. Reg. No. Pt. Status Last follow up Before treatment report After treatment report Unknown to CT Abdomen ( ): FOC of Ca GB showing a single enlarge mesenteric lymph nodes (2.3x1.7 cm) medial to hepatic flexure. CT Abdomen ( ): FUC of Ca GB showing a single enlarged msenteric lymph nodes (2.3x1.7 cm). Medial to hepatic flexure. Compared to previous scan dated there is no radiological improvement.

58 Ca. Gall Bladder S. N o. Reg. No. Pt. Status Last follow up Before treatment report After treatment report Unknown to CA-19.9 : 48.6 U/ML ( ) CT whole Abdomen ( ): CT reveals multiple peripherally enhancing hypodense hepatic SOL's (largest 9.5 x 6.9 cm). D/D multi centric HCC's. U/S secondaries. CA-19.9: 29.6 u/ml ( ) USG Whole Abdomen ( ): Multiple SOL (40 mm to 80 mm) in liver. Gall bladder not seen due to SOL.

59 Ca. Gall Bladder S. N o. Reg. No. Pt. Status Last follow up Before treatment report After treatment report Unknown to USG Abdomen ( ): Hepatomegaly in echogenic lesion (46x31 mm) with embedded calculi in GB lumen envolving mid CBD, causing dilated proximal CBD and moderate IHBR dilatation - ? Ca. GB. CT Abdomen ( ): Chronic calculus cholecystitis with suspected carcinoma GB causing extra hepatic billiary obstruction an hepatic infilteration. USG Whole Abdomen ( ): Cholecystitis with choledo choledocholithiasis. Normal pelvic study. No mass seen in GB.

60 Ca. Gall Bladder S. N o. Reg. No. Pt. Status Last follow up Before treatment report After treatment report 49865Unknown to USG Abdomen ( ): Impression: Mass (27x28 mm) in pancreatic head region adenopathy. No billary channel dilatation bneumobila. USG Whole Abdomen ( ): Normal size liver shows slightly dilated intrahepatic billiary channel. Gall bladder could not visualized. CBD is normal in calibre. A hypoechoic solid mass lesion (20.8x18.1 mm) in head of pancreas. Spleen and Kidneys are normal in size. CT upper abdomen ( ): Post Cholecystectomy case reveals mild hepatomegaly with mild IHRD dilatation with pneumobila dilated CBD. Partial gastric outlet obstruction. Pancrease is normal in size and contour.

61 Ca-Colon S. N o. Reg. No. Pt. Status Last follow up Before treatment report After treatment report Unknown to CT Abdomen ( ): FUC of carcinoma colon reveals multiple irregular heterogeniously enhanding soft tissue density lesions adjacent to ileostomy site, along post operative scar, greater curvature of stomach and ill mesentery S/o metastatic deposits irregular soft tissue thickening is seen around right prosterio lateral margin of uterus with indistinct fat planes with rectum posteriorly, likely S/o recurrence of the disease. Multiple small paraaortic and paracaval lymphnodes are seen. CA-19.9 : 29.4 u/ml ( ). CT Abdomen ( ): Irregular heterogeniously enhanding soft tissue density lesions adjacent to ileostomy site, along post operative scar, greater curvature of stomach and ill mesentery S/o metastatic deposits remains grossly the same. Irregular soft tissue thickening around right prosterio lateral margin of uterus with indistinct fat planes with rectum posteriorly, likely S/o recurrence of the disease with no interval changes. Multiple small subcentimeter paraaortic and paracaval lymphnodes are seen. There is no ascitis. CA-19.9: 4.7 u/ml ( )

62 CA Breast S. N o. Reg. No. Pt. Status Last follow up Before treatment report After treatment report Unknown to CA -15.3: 38.2 u/ml ( ) CA-15.3: 13.7 u/ml ( ) Unknown to CA -15.3: u/ml ( ) CA-15.3: 3.4 u/ml ( ) Unknown to CA-15.3: u/ml ( ) CT Thorax ( ): Right axillary adenopathy with ulceration in right breast soft tissue and left basal atelectasis. CA-15.3: ( ) CA-15.3: u/ml ( ).

63 CA Breast S. N o. Reg. No. Pt. Status Last follow up Before treatment reportAfter treatment report Unknown to Mammography ( ): The right breast is normal in size. Fare amount of fatty and little glandular tissue seen in right breast nipple is retracted and shows radiopaque mass (4x3.5 cm) with irregular margins in sub areolar lesion. No abnormal focal mass is seen in left breast. CA-15.3: 31.3 u/ml ( ) Sonomammography ( ): Large poorly marginated hypoechoic SOL (2.7x3.1x3.2 cm) in right breast. A small (9x7 mm) echogenic lesion of oval shape is seen in right breast (? Lipoma). Sonomammography ( ): Hypoechoic pooraly marginated SOL (2.8x2.7x2.1 cm) in right breast with spaculated margin S/o neoplastic etiology. Sonomammography ( ): Hypoechoic poorly marginated right breast SOL (approx 2.1 x 1.8 cm). Reactive right axillary nodes.

64 CA Breast S. No. Reg. No. Pt. Status Last follow up Before treatment reportAfter treatment report Unknown to CA-15.3: 21.5 u/ml ( ) CT reports are not available CA-15.3: 18.5 u/ml ( ) CT Thorax ( ): CT reveals large SOL (5.4x6.8 cm) in right breast ? Malignancy. Mammography ( ): Large hypoechoic SOL (6.16x4.62x5.27 cm) right breast with necrosis and axillary lymphadenopathy.

65 RESULTS ON HEPATITIS B & C In so many cases of Hepatitis B (HBSAg) and Hepatitis C ( HCV) our Herbal and Herbo-mineral treatment respond to decrease Viral load upto the Not detectable results. Patients Health improved and sufferings minimized.

66 RESULTS ON AIDS In HIV infection Viral load increases. In AIDS Viral load increases and CD4 Count decreases. We observed that after our Herbal and Herbo-mineral treatment CD4 count increased and Viral load decreased. So it minimizes the possibility of AIDS. They live Happy Life.

67 Body Management of the body ailments by Purification & de-toxification upto cell level by Panchkarma & treatment by Herbs and Herbo-mineral drugs

68 Mind Management of senses- (1)Vision,(2) Hearing,(3) Smell,(4) Taste, (5) Touch. Management of Chakras which are seven in numbers.. Diagnosis by Aura Imaging.. Management by Frequency of opposite Polarity.

69 Treatment Is Given By 1-Crystal Therapy2-Color Therapy 4-Acupuncture5-Aroma Therapy6-Music Therapy 3-Acupressure

70 Treatment Is Given By 7 - Reiki 9-Yoga 8-Pranayam

71 Soul By Meditation for connection with God By Prayer Forgiving us & Thankful to God By Donation (ZAKAT) for Universal Service and Humanity.

72 Thats why potential of Lavanya Ayurveda is treatment of disease at cell level or Root cause of Disease so we can rejuvenate and repair the cells.

73 The problems of Ageing and incurability of disease can be solved.

74 Ayurvedas priority is how to be disease free by adopting correct diet and life style in detail so that chances of disease are ruled out.

75 Not only according to the individual body constitution but also according to the relationship between seasonal changes, day to day time management.

76 To be free from Tension & Emotional Disorders * * W.H.O. QUOTES FOR A HEALTHY PERSON

77 Thats why Lavanya Ayurveda has approached a step forward by integration with modern medicine which is necessary for wellness of Humanity.

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79 THANK YOU! Visit Us: Mail Us: Call Us:


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