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Are We There Yet? Zebrafish Nutrition Stephen A. Watts, Louis D Abramo, Susan Farmer, Lacey Dennis, Daniel Smith and Mickie L. Powell University of Alabama.

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Presentation on theme: "Are We There Yet? Zebrafish Nutrition Stephen A. Watts, Louis D Abramo, Susan Farmer, Lacey Dennis, Daniel Smith and Mickie L. Powell University of Alabama."— Presentation transcript:

1 Are We There Yet? Zebrafish Nutrition Stephen A. Watts, Louis D Abramo, Susan Farmer, Lacey Dennis, Daniel Smith and Mickie L. Powell University of Alabama at Birmingham and Mississippi State University

2 Why Nutrition? ZF have been used extensively as a drug and disease model ZF are a useful toxicology model Despite their widespread use the nutritional requirements have not been determined

3 Aquaculture- Grow large fish fast. Aquarists- Color, Reproduction Research- Growth, Overall health, High fecundity.

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5 Ingredients are practical Possible anti- nutritional factors No indication of quantities Use of lakes and dyes Use of preservatives

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7 There are no standards among diets in zebrafish studies!!

8 Where do we start?

9 Examples of Nutrition Studies

10 Considerations in the Use of Formulated Diets Age: Larval vs juvenile vs adult (breeding) Physical form of the diet Feed Frequency Protein source/quality Carbohydrate Lipid and Fatty Acids Gut Retention Time Bacteria Culture Container

11 Methods Fish were co-cultured with enriched rotifers for the first 5 days post hatch and fed Artemia for 11 days prior to stocking. At 21 days post hatch 15 fish were stocked randomly into 2.8 liter tanks and maintained on a recirculating zebrafish system. For each diet, fish were fed a ration in excess of 5% of their body weight per day, divided into two feedings. Fish were photographed and weighed every two weeks to measure growth and adjust feed rations.

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13 UAB Z-12 Diet Formulation Ingredient% casein - vitafree25.00 fish protein hydrolysate20.00 wheat starch9.60 wheat gluten7.00 alginate5.38 soy protein isolate5.00 dextrin5.00 menhaden fish oil4.67 soy lecithin (refined)4.00 vitamin premix4.00 mineral premix3.00 corn oil2.33 canthaxanthin (10%)2.31 potassium phosphate monobasic1.15 alpha cellulose1.00 glucosamine0.25 betaine0.15 cholesterol0.12 ascorbylpalmitate0.04 Total100.00

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17 Week 10 Artemia Gemma

18 DietBody Condition Index Artemia0.98 Gemma1.09 Z Ziegler1.24 Otohime1.24 Tetramin1.32 High BCI Low BCI

19 Can one nutrient affect experimental outcomes?

20 Protein Mixed source vs sole source

21 Amino Acid Analysis MIXEDFPICASSOYWG Cysteine Methionine Lysine Alanine Arginine Aspartic Acid Glutamic Acid Glycine Isoleucine Leucine Serine Threonine Valine Histidine Phenylalani ne Tyrosine Taurine Tryptophan

22 µCT of Mixed Protein and Soy Protein Diets

23 Bone Alterations

24 Conclusions All diets supported growth and survival. However, commercial diets contain undefined ingredients. The consequence: Nutrients and nutrient source affect outcomes. Any health or disease-related outcomes will be affected by diet. Interpretation of experimental results must be made within the context of a defined nutritional history, or lack thereof. Microbiome?

25 Acknowledgments UAB NORC Aquatic Animals Research Core (NIH P30DK056336). Jeff Barry, Adele Fowler, Chris Taylor, Michael Williams, Karen Jensen, Marlee Hayes ACLAM


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