1. Prolonged gonadotropin-releasing hormone agonist therapy reduced expression of nitric oxide synthase in the endometrium of women with endometriosis and infertility 
JianHua Wang, M.D., FuZhen Zhou, M.D., MinYue Dong, M.D., RuiJin Wu, Ph.D., M.D., YuLi Qian, Ph.D. 
Fertility and Sterility 
Volume 85, Issue 4, Pages (April 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Representative Western immunoblot for eNOS in the endometrium during various stages of the proliferative phase (A) and the secretory phase (B). Lanes 1–3: patients with endometriosis at early, middle, and late stages, respectively; lanes 4–6: patients with endometriosis after treatment with GnRH-a at early, middle, and late stages, respectively; lanes 7–9: control women at early, middle, and late stages, respectively. Detection of β-actin protein was used as an internal control in parallel experiments. (C and D) The summary densitometric analysis of eNOS protein levels (ratio to β-actin) of these data during the early, middle, and late proliferative phases, and the early, middle, and late secretory phases. Box and whisker plots: bar (horizontal line) = median; box = 25th to 75th percentiles; whiskers = extend to the extreme values. *P<.05 and **P<.01 when compared with patients with endometriosis in the respective menstrual stage. Diagonal lines = endometriosis group; crosshatched lines = GnRH-a–treated group; unshaded area = control group.
Wang. GnRH-agonist and NOS in endometriosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
XY-scatterplots of endometrial eNOS protein relative expression and serum E2 or P levels in all subjects of the three groups. Each dot represents a specific sample. Data are given in a linear scale. (A) Correlation between serum E2 and endometrial eNOS protein relative levels(r = in G1, r = in G2, and r = in G3; all subjects, r = 0.612; all P<.01, n = 67). (B) Correlation between serum P and endometrial eNOS protein relative levels(r = in G1, r = in G2, and r = in G3; all subjects, r = 0.647; all P<.01, n = 67). G1 = endometriosis patients; G2 = GnRH-a–treated patients; G3 = control patients.
Wang. GnRH-agonist and NOS in endometriosis. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions