1. A Truncated Arabidopsis NUCLEOSOME ASSEMBLY PROTEIN 1, AtNAP1;3T, Alters Plant Growth Responses to Abscisic Acid and Salt in the Atnap1;3-2 Mutant 
Liu Zi-Qiang , Gao Juan , Dong Ai-Wu , Shen Wen-Hui  
Molecular Plant 
Volume 2, Issue 4, Pages (July 2009)
DOI: /mp/ssp026
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
BiNGO Analysis of Down-Regulated Genes in the Atnap1 Triple Mutant m123-2 from the ATH1 Microarray Experiment.
The yellow to orange color of the circles correspond to the level of significance of the overrepresented GO category from and below according to a multiple t-test with false discovery rate-corrected p-value. The size of the circle is proportional to the number of genes in the category.
Molecular Plant 2009 2, DOI: ( /mp/ssp026)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

3. Figure 2
Effects of ABA on Seed Germination and Seedling Growth of the Atnap1 Triple Mutants m123-1 and m123-2 Compared with Wild-Type Col.
(A) A representative photograph of Col, m123-1 and m123-2 seedlings grown on medium containing 2 μM ABA, 18 d after seed sowing.
(B) Representative seedlings of different genotypes taken from (A). The arrowheads indicate separation of seedlings scored as growth (those on the left side) and arrested (those on the right side).
(C) Germination rate of each genotype on medium containing 2 μM ABA. Each point represents the mean value from three replicates with more than 90 seeds each. Bars represent standard deviations.
(D) Time course of seedling growth of different genotypes on medium containing 2 μM ABA. Each point represents the mean value from three replicates containing more than 90 seeds each. Bars represent standard deviations.
Molecular Plant 2009 2, DOI: ( /mp/ssp026)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

4. Figure 3
Effects of ABA on Atnap1 Single, Double, and Triple Mutants Containing or Not the Atnap1;3-2 Allele.
(A) A representative photograph of seedlings of Col, m1, m2, m3-2, m12, m13-2, m23-2, and m123-2 grown on medium containing 2 μM ABA, 12 d after seed sowing.
(B) Seedling growth of different genotypes on medium containing 2 μM ABA, as measured 10 d after seed sowing. Each column represents the mean value from three replicates containing more than 90 seeds each. Bars represent standard deviations.
(C) Representative seedlings of different genotypes grown on medium containing 2 μM ABA, 12 d after seed sowing.
Molecular Plant 2009 2, DOI: ( /mp/ssp026)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

5. Figure 4
Comparison of Stomata Closure Induced by ABA in Atnap1 Mutants and in Wild-Type.
(A) Representative images of stomata of wild-type Col and the m123-2 mutant after treatment with 0 or 5 μM ABA. Bars  =  2 μm.
(B) Stomata aperture of wild-type Col and mutants (m1, m2, m3-2, and m123-2) measured after treatment with 0 or 5 μM ABA. Each column represents the mean value from more than 110 stomata. Bars represent standard deviations.
Molecular Plant 2009 2, DOI: ( /mp/ssp026)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
Effects of Salt Stress on Atnap1 Mutants Compared with Wild-Type Col.
(A) A representative photograph of seedlings of Col, m123-1, and m123-2 grown on medium containing 150 mM NaCl, 18 d after seed sowing.
(B) Time course of seedling growth of Col, m123-1, and m123-2 on medium containing 150 mM NaCl. Each point represents the mean value from three replicates containing more than 90 seeds each. Bars represent standard deviations.
(C) Seedling growth of different genotypes on medium containing 150 mM NaCl, 24 d after seed sowing. Each column represents the mean value from three replicates containing more than 90 seeds each.
Molecular Plant 2009 2, DOI: ( /mp/ssp026)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

7. Figure 6
Detection of the Truncated Protein AtNAP1;3T in Different Atnap1 Mutants and Binding of AtNAP1;3T with AtNAP1 and H2A Proteins.
(A) Northern-blot analyses of expression of AtNAP1 genes in wild-type Col and different Atnap1 mutants using AtNAP1;1, AtNAP1;2, and AtNAP1;3 cDNA probes. Positions of wild-type and truncated transcripts are indicated by arrowheads.
(B) Western-blot analyses of AtNAP1 proteins in wild-type Col and different Atnap1 mutants. The Coomassie stained protein gel (upper panel) and the corresponding Western image (bottom panel) were shown. Positions of wild-type AtNAP1;1, AtNAP1;2, and AtNAP1;3 as well as the truncated protein AtNAP1;3T are indicated.
(C) Pulldown assay. Total protein extracts of transgenic Arabidopsis plants expressing YFP-AtNAP1;2, YFP-AtNAP1;3, or H2A-YFP were incubated with beads coated with 6xHis-AtNAP1;3, 6xHis-AtNAP1;3T, or 6xHis-AtNRP1 and the pulldown fractions were analyzed by Western blot using a polyclonal anti-GFP antibody (which cross-reacts with YFP). The input was loaded as control.
Molecular Plant 2009 2, DOI: ( /mp/ssp026)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

8. Figure 7 AtNAP1;3T Is a Negative and Dominant Factor in ABA Response.
(A) A representative photograph of wild-type Col, homozygous m3-2, and heterozygous m3-2+/– mutant seedlings grown on medium containing 2 μM ABA, 18 d after seed sowing.
(B) Time course of seedling growth of wild-type Col, homozygous m3-2, and heterozygous m3-2+/– mutants on medium containing 2 μM ABA. Each point represents the mean value from three replicates containing more than 90 seeds each. Bars represent standard deviations.
(C) A representative photograph of 14-day-old seedlings grown on medium containing 2 μM ABA of wild-type Col, homozygous m3-2, and transgenic lines (OE1 to OE6) expressing YFP-AtNAP1;3T under the control of the CaMV 35S promoter.
(D) Seedling growth of different lines on medium containing 2 μM ABA, 10 d after seed sowing.
(E) Quantitative RT–PCR analysis of the expression of ABA-responsive genes. Total RNA was prepared from about 100 seedlings of 6-day-old and used for RT–PCR analysis. The analysis was performed in two independent experiments. The mean values of the relative expression levels of genes in the m123-1 and m123-1 mutants and in the transgenic line OE1 overexpressing YFP-AtNAP1;3T compared with wild-type plants are shown by columns, and the standard deviations are indicated by the error bars.
Molecular Plant 2009 2, DOI: ( /mp/ssp026)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions