1. Hyaluronan Synthase Induction and Hyaluronan Accumulation in Mouse Epidermis Following Skin Injury 
Raija Tammi 
Journal of Investigative Dermatology 
Volume 124, Issue 5, Pages (May 2005)
DOI: /j X x
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2. Figure 1
Hyaluronan and CD44 expression in developing and adult mouse epidermis. Sections of mouse skin were stained for hyaluronan (a–i) and CD44 (j–m,) as described in Materials and Methods. Micrographs of fetuses were taken from the head area (a–c, j–l), and micrographs from newborn and adult mice were taken from the ear (g) and tail (d–f, h, i, m). Arrows indicate the locations of the basal lamina, and the asterisk in (i) the inflamed epidermis. Scale bars=10 μm.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Visual scoring of epidermal hyaluronan staining. The staining was scored as follows: 0=no staining in any area (white section of the column), 1=localized staining in the epidermis surrounding hair infundibulum in 1–2 follicles (light gray section of the column); 2=localized staining in the epidermis surrounding hair infundibulum in less than 50% of follicles (dark grey section), 3=localized staining around hair infundibulum in more than 50% of hair follicles and/or in the interfollicular epidermis (black section). Tail sample numbers: newborn (0 mo) n=6; 1 mo n=6; 2–5 mo n=14; over 7 mo n=13. Ear sample numbers: 1 mo old n=8; 2–5 mo n=9; over 7 mo n=5.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Hyaluronan and CD44 in tape-stripped mouse epidermis. Mouse tail skin was traumatized with repeated tape stripping and analyzed after 1 d (a, b), 3 d (c–k), and 7 d (l), and stained for hyaluronan (a–h, l) and CD44 (i–k), as described in Materials and Methods. Traumatized area is on the left side of the dashed line in (a) and (d), and is shown at higher power in (e) and (i). Adjacent, non-traumatized area on the right side of the dashed line in (a) and (d) is shown at higher magnification in (b), (c), (f), (g), and (j). An area approximately 1.5 mm away from the trauma is shown in (h) and (k). Asterisks (d, e, i, l) indicate the eschar. Arrows (d, e, i) indicate the migrating cells. Scale bar=50 (a–d, l) and 20 μm (others).
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Hyaluronan concentration and hyaluronan synthase and CD44 mRNA levels in tape stripped mouse epidermis. (a) Mouse tail skin was repeatedly tape stripped, and specimens were taken after 1–10 d. Epidermis was separated from dermis with ethylenediamine tetraaceticacid, and the hyaluronan content was measured using an enzyme-linked sorbent assay. The data represent means and SE of untreated controls (day 0, n=40), and tape-stripped animals (day 1, n=2; day 2, n=6; day 3, n=8; day 7, n=3; and day 10, n=3). Analysis of variance showed that the stripping caused a statistically significant increase in the epidermal hyaluronan content (p<0.001), day 3 value being significantly higher than control or day 10 value (Scheffe's test, p<0.001 and 0.01, respectively). (b) RNA was extracted from the epidermis of E18 fetal, newborn, and adult mouse, and adults 3 d after tape stripping. RT-PCR was performed with primers specific for the different has isoforms, and CD44, and related to the band density of glyceraldehyde phosphate dehydrogenase (GAPDH). has1 did not produce a significant signal in any of the samples. The data represent means and ranges from two to five specimens.
Journal of Investigative Dermatology  , DOI: ( /j X x)
Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions