1. Peritumoral injections of poly(I:C) induce type I IFN–dependent cytotoxic immunity and delay the growth of primary and transplanted Hgf-Cdk4R24C melanomas.
Peritumoral injections of poly(I:C) induce type I IFN–dependent cytotoxic immunity and delay the growth of primary and transplanted Hgf-Cdk4R24C melanomas. Primary DMBA-induced (A–F) and intracutaneously (i.c.) transplanted (F–J) Hgf-Cdk4R24C melanoma model. A and F, experimental protocols for short-term treatment of melanomas with poly(I:C). B and G, fold increase of mRNA expression levels for the indicated genes (left) and of the percentage of tumor-infiltrating immune cells (right) in primary and transplanted Hgf-Cdk4R24C melanomas compared with controls (mean ± SEM; n = 6). C and H, experimental protocols for long-term treatment of melanomas with poly(I:C). D and I, tumor growth kinetics of melanomas in individual mice treated as indicated (left, middle), and mean survival in each cohort (right; mean ± SEM; n = 6; **, P < 0.01). Similar results were obtained in two independent treatment cohorts. E and J, corresponding tumor growth kinetics and mean survival in Ifnar1-deficient cohorts of mice treated as indicated (n = 6). Similar results were obtained in two independent treatment cohorts.
Tobias Bald et al. Cancer Discovery 2014;4:
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