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Master course Molecular & Cellular Life Sciences Part 1: Intracellular Membrane Processes October 8, 2009 Paul van Bergen en Henegouwen
Mechanisms of clathrin-independent internalization Paul van Bergen en Henegouwen
Different endocytic portals
Endocytosis: whats the problem? bendingfission
Five ways to bend a membrane
Clathrin-coated and vesicles: Ultrathin section of plastic-embedded material
Clathrin-coated pits and vesicles by EM Replica of freeze-dried plasma membranes
Clathrin-coated vesicles: Replica of freeze-dried plasma membranes
Clathrin: Heavy- and light chains
Clathrin makes a network
How clathrin is bending?
AP-2 Adaptor Protein-2 complex a b2b2 m2m2 s2s2 Ear Hinge Trunk 100 kDa 50 kDa 17 kDa a-adaptin b-adaptin
There are different AP-complexes on different sites in the cell Golgi-complex Plasma membrane Golgi-complex ab2b2 m2m2 s2s2 g b1b1 m1m1 s1s1 d b3b3 m3m3 s3s3 AP-1 AP-2 AP-3
Protein/protein interactions control CCV formation a b2 m2 s2 Receptor Yxxf Eps15 DPF Receptor LL L fxf D/E Clathrin AP-2 I EHs II COIL III DPF/UIM Epsin NPF Lipids DPW PIP 2
Epsin induces bending of liposomes
The netwerk forms a ball Electron tomography of a coated vesicle Adaptor protein (AP2) complex s chainm chain b adaptina adaptin Clathrin triskelion Smith et al. (1998) EMBO J. 17, 4943
Overview of clathrin structures Marsh and McMahon (1999) Science 285,
From bending to fission
Dynamin, a GTPase involved in fission PIP 2 Amphiphysin
Dynamin is forming a ring around the neck
Effect of pure dynamin on lipid vesicles
PIPK Membrane curvature generation AP2 Clathrin Eps15 Intersectin Amphiphysin Epsin Dynamin Endophilin Moleculen involved in the whole process
Possible role for actin filaments
PIPK Membrane curvature generation AP2 Clathrin Eps15 Intersectin Amphiphysin Epsin Dynamin Endophilin Actin Synaptojanin Auxilin Hsc70 Moleculen involved in the whole process
Overview of all interactions
EGFR internalization via a non-coated pathway!
Is the alternative route visible?
Caveolar internalization starting from cholesterol-rich domains
Trafficking of Caveolae
Phagocytosis: Internalization of large items
Overview of different internalization routes
Properties of different CI-routes
Classification of internalization routes
Questions What does the bending? What does the fission?
Interleukin-2 receptor complex
IL-2R internalization is Clathrin-independent clathrin-inhibition by ΔEps15
IL-2R internalization is Dynamin-dependent Dynamin-inhibition by Dyn-K44 TfR wt K44A
IL-2R is present in the Detergent Resistant Membrane (DRM) fraction
Master class Biomolecular Sciences Part 1: Molecular Cell Biology. 4 September 2007 Paul van Bergen en Henegouwen.
Discovery of New Cargo Proteins that Enter Cells through Clathrin-Independent Endocytosis Craig A. Eyster, Jason D. Higginson, Robert Huebner, Natalie.
Chapter 15 Intracellular Compartments and Transport Essential Cell Biology Third Edition Copyright © Garland Science 2010 Hilary Truchan
1 Chapter 7 Intracellular Trafficking Copyright © 2012, American Society for Neurochemistry. Published by Elsevier Inc. All rights reserved.
Chapter 13 Intracellular Vesicular Traffic 張學偉 助理教授.
Homeostasis depends in part on appropriate movement of materials across the cell membrane. Homeostasis refers to the necessity of an organism to maintain.
PHOSPHOLIPIDS … Hydrophilic molecules are attracted to water. Hydrophobic molecules are not attracted to water but to each other. Phospholipid molecules.
Lecture #7 Chapter 15 Cell signaling: Communication Between Cells and Their Environment Axiom #7 Study hard and stay focused on your educational goals.
Chapter 6 A Tour of the Cell. You should be able to: 1.Distinguish between the following pairs of terms: magnification and resolution; prokaryotic and.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece.
How does the ParABC system segregate low copy number plasmids in bacteria? Martin HowardDept of Systems Biology John Innes Centre Norwich, UK.
Topic 5 Antigen Processing ©Dr. Colin R.A. Hewitt
Pathways that Harvest and Store Chemical Energy 6.
The Cell The Basic Unit of Life. The Importance of Cells All organisms are made of cells The cell is the simplest collection of matter that can live.
Cell Communication Cells need to communicate with one another, whether they are located close to each other or far apart. Extracellular signaling molecules.
Section 3-3, 3-4, 3-5 Movement across the c ell membrane Write everything that is underlined.
Microscopes provide windows to the world of the cell.
Cell The smallest unit of life that can perform all life processes.
Questions for lab 1.Halobacterium is an extremophile. In which conditions is it able to grow? In which conditions does it grow best? 2.In what ways do.
Target VII Identify and describe the functions of the component structures of the plasma membrane: phospholipid, integral protein, peripheral protein,
The Cell Factory. ORGANELLE is a specific structure of a cell having specialized functions. is a specific structure of a cell having specialized functions.cell.
1 Chapter 25 Serine and Threonine Phosphorylation Copyright © 2012, American Society for Neurochemistry. Published by Elsevier Inc. All rights reserved.
BIOLOGY CST Review PowerPoint Day 1: Standards. 1a -1h.
Chapter 7 Membrane Structure and Function. You should be able to: 1. Define the following terms: amphipathic molecules, aquaporins, diffusion 2. Explain.
Claim: because extant organisms share processes and structures it indicates that they evolved from a common ancestor (similarity implies ancestry). Essential.
The endoplasmic reticulum and the Golgi apparatus permit protein construction and transport in a cell. 11/20 How are cells are divided into organelles.
SIGNAL TRANSDUCTION A. OVERVIEW OF RECEPTORS AND SIGNALLING RECEPTOR FAMILIES MECHANISMS OF SIGNALLING BY RECEPTORS AMPLIFICATION OF SIGNALS KEY FUNCTION.
1 Chapter 12 Intercellular Signaling Copyright © 2012, American Society for Neurochemistry. Published by Elsevier Inc. All rights reserved.
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings PowerPoint ® Lecture Presentations for Biology Eighth Edition Neil Campbell.
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