1. Fig. 3. Wisper controls CF behavior and survival.
Wisper controls CF behavior and survival. (A) Wisper expression in adult CFs after GapmeR transfection at increasing concentrations. Bars represent means normalized to control ± SEM (n ≥ 6). P values were determined by Student’s t test. (B and H) Gene expression measured by qRT-PCR in adult CFs (B) or lung fibroblasts (FBs) (H) after transfection with GapmeRs (10 nM, 48 hours; n ≥ 5) targeting Wisper (GM-Wisper) or scrambled GapmeRs (GM-Scr). P values were determined by two-way ANOVA (Fisher’s test). (C and I) Proliferation of adult CFs (C) or lung fibroblasts (I) after GapmeR transfection (10 nM) quantified by [H3]thymidine incorporation. Data are expressed as mean counts per million (cpm) ± SEM (n ≥ 3). P values were determined by two-way ANOVA (Bonferroni’s test). (D and J) Migration of adult CFs (D) or lung fibroblasts (J) after GapmeR transfection (10 nM) measured using a wound closure assay. Representative pictures are shown. Scale bars, 100 μM. Graphs show the percentage of wound closure at each time point ± SEM (n ≥ 3). P values were determined by two-way ANOVA (Bonferroni’s test). (E and K) Representative fluorescence-activated cell sorting (FACS) analysis of annexin V–positive adult CFs (E) or lung fibroblasts (K) after GapmeR transfection (10 nM). Graphs show the percentage of apoptotic cells ± SEM (n = 3). P values were determined by Student’s t test. (F) Bax expression–to–Bcl2 expression ratio in adult CFs after GM-Wisper or GM-Scr transfection (10 nM, 48 hours). Bars represent means ± SEM (n = 3). P values were determined by Student’s t test. (G) Wisper expression in adult CFs and lung fibroblasts after GM-Wisper transfection (10 nM). Bars represent means normalized to GM-Scr ± SEM (n ≥ 5). P values were determined by Student’s t test.
Rudi Micheletti et al., Sci Transl Med 2017;9:eaai9118
Published by AAAS