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Homeostatic γδ T Cell Contents Are Preserved by Granulocyte Colony-Stimulating Factor Priming and Correlate with the Early Recovery of γδ T Cell Subsets after Haploidentical Hematopoietic Stem Cell Transplantation Zhilei Bian, Lan-Ping Xu, Qiang Fu, Mingrui Huo, Long Liu, Xiaosu Zhao, Xiao-Jun Huang, Jiangying Liu Biology of Blood and Marrow Transplantation Volume 24, Issue 2, Pages (February 2018) DOI: /j.bbmt Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions
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Figure 1 Representative images of immunophenotyping for γδ T cells and their subsets. Flow cytometry assay showed the phenotypic images of γδ T cells (A), CD27+ and CD27- fractions of γδ T cells (B), Vδ1+ and Vδ2+ fractions of γδ T cells (C), CD27+ and CD27- fractions of Vδ1 cells (D), and CD27+ and CD27- fractions of Vδ2 cells (E). Biology of Blood and Marrow Transplantation , DOI: ( /j.bbmt ) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions
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Figure 2 Comparisons of the cytokine production in γδ T cells and their subsets. The production of IFN-γ and IL-17 in response to PMA stimulation was compared in total γδ T cells (A), CD27+γδ T cells (B), CD27-γδ T cells (C), Vδ1 cells (D), and Vδ2 cells (E). All P < .001, n = 6. Biology of Blood and Marrow Transplantation , DOI: ( /j.bbmt ) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions
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Figure 3 Correlation analyses of the γδ T cell contents between healthy donors and haploHSCT recipients. The scatter spots indicate the proportions of Vδ1 and Vδ2 fractions in peripheral γδ T cells. (A-D) Correlation of the Vδ1 concentration in γδ T cells between donors and recipients at 30, 60, 90, and 180 days after haploHSCT. (E-H) Correlation of the Vδ2 content in γδ T cells between donors and recipients at 30, 60, 90, and 180 days after haploHSCT. Significance levels of the bivariate correlations were analyzed by the Spearman test. P values are presented on the graphs, n = 40. Biology of Blood and Marrow Transplantation , DOI: ( /j.bbmt ) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions
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