Presentation on theme: "What Are You Drinking, TEA? Tira J. Robinson Junior Biology Health Science Major Tennessee Technological University April 27, 2005."— Presentation transcript:
What Are You Drinking, TEA? Tira J. Robinson Junior Biology Health Science Major Tennessee Technological University April 27, 2005
*Project Summary To see what type of bacteria and e-coli grows in different teas To see how many coliforms grow from tea samples Take several different tea samples and run tests Take a count of how many coliforms were present at the end of the experiment. Give data and charts on results
Introduction There have been several media reports on the health hazards of fecal coliform contamination of restaurant brewed iced tea ( Doyle 1996). In January the CDC issued an alert stating that ice tea can breed bacteria if it sits out all day (Kapner 1996). There may be microbes, but they probably are of the relatively harmless variety that naturally cling to tea leaves (Nutrition 1996). The Centers for Disease Control and Prevention, which investigated the reports about iced tea, explained that theoretically tea could transmit disease if other bacteria survived the brewing (Iced 1996). The large numbers of relatively benign bacteria that showed up probably occurred because of lax sanitation of restaurant tea dispensers (Nutrition 1996).
Objective Statement The objective for this experiment is to se what type of bacteria and microorganisms grow in different tea samples, if in they do, whether e-coli or metallic sheen is present.
Hypothesis Statement I hypothesize that microorganisms do grow in tea samples due to the tea leaves and or the amount of time tea is left sitting.
Methods & Materials Pour Plate method on TSA & Endo Agar Plates Each plate will have a different measurement of.1,.5, 1 mL Coliform count Incubation of the plates for growth Tea Samples from 3 different restaurants Data will be recorded in tables This experiment will be administered by Dr. Goss
Results Endo Agar Plate Results After incubation I found in restaurant A1 that it contained metallic sheen with fecal colifroms, which is e-coli. All of restaurant A had grown mold B1 also contained metallic sheen and fecal coliforms. C1 only contained metallic sheen
Results Cont. TSA plate results All of the 3 restaurants contained fermentation See Chart For More Details
Data Endo Agar Plates Ex. mL. Restaurant ARestaurant BRestaurant C colonies252 colonies 372 colonies colonies54 colonies 500 colonies colonies 26 colonies 710 colonies Comments: A1 contained metallic sheen with fecal coliforms. A2 contained only fecal coliforms (e-coli). All of A had mold. B1 contained metallic sheen and fecal coliforms. C1 only contained metallic sheen. TSA Plates Ex. mL.Restaurant ARestaurant BRestaurant C colonies9 colonies400 colonies 2113 colonies14 colonies800 colonies Comments: All restaurant samples contained fermentation
Literature Cited Doyle, Michael. Food Technology. Fecal conditions in tea: whats the problem. Oct v50 n10 p104(1) Environmental Nutrition. Iced tea scare a tempest in a teapot. June 1996 v19 n6 p8(1) Environmental Nutrition. Iced tea scare a tempest in a teapot. June 1996 v19 n6 p8(2) Kapner, Suzanne. Iced-tea brewhaha: CDC says drink OK. June 1996 v30 n23 p1(2) Marcinkowski, Victoria. Coffee, tea, or bacteria? Jan v59 i8 p6(2)
Conclusion There are bacteria present in tea. For the most part it isnt harmful Some of the causes can be from the tea leaves and or the time it has sat in high temperatures. The growth of e-coli can be harmful You can keep drinking tea!!!!!
Acknowledgements I would like to thank Dr. Goss for her time and help A special thank you to Cara Sanders for an outstanding hand. A special thanks to Kimberly Tuggle for being a great partner. Thank you Dr. Morgan for all the help