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Activity-dependent tuning of inhibitory neurotransmission based on GABAAR diffusion dynamics Hiroko Bannai, Sabine Lévi, Claude Schweizer, Takafumi Inoue,

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Presentation on theme: "Activity-dependent tuning of inhibitory neurotransmission based on GABAAR diffusion dynamics Hiroko Bannai, Sabine Lévi, Claude Schweizer, Takafumi Inoue,"— Presentation transcript:

1 Activity-dependent tuning of inhibitory neurotransmission based on GABAAR diffusion dynamics
Hiroko Bannai, Sabine Lévi, Claude Schweizer, Takafumi Inoue, Thomas Launey, Victor Racine, Jean-Baptiste Sibarita, Katsuhiko Mikoshiba and Antoine Triller Neuron, 2009 Céline Devroye M2 Neurosciences

2 INTRODUCTION Modulation of synaptic strength Learning and memory
Depends on : • Number of functional release sites • Release probability • Post-synaptic response => occurs at both excitatory and inhibitory synapses What molecular and cellular mecanisms in inhibitory synapses? GABAergic synapse

3 INTRODUCTION GABAERGIC SYNAPSE
Hippocampus : inhibitory transmission by GABA and GABAA receptors GABAAR GABA Gephyrin Amount of GABAAR (post synaptic response) Strength of synapse Correlation between changes in GABA currents and GABAAR amounts regulation of amount of receptor at the synapse - Lateral diffusion? - AMPA lat diff are activity dependent - Calcium? Membrane insertion, removal and lateral diffusion number of GABAAR at the synapse Neuronal activity surface expression of GABAAR

4 Does neuronal activity regulates the diffusion dynamics of GABAAR?
QUESTION INTRODUCTION Does neuronal activity regulates the diffusion dynamics of GABAAR?

5 EXPERIMENTAL APPROACH
INTRODUCTION Hippocampal neurons (culture) Neuronal activity : Amounts of GABAAR Amplitude of GABA currents Diffusion dynamics of GABAAR

6 NEURONAL ACTIVITY - GABAAR AND GEPHYRIN CLUSTER SIZES
RESULTS Gephyrin: scaffolding protein of GABAAR  Immunofluorescence • GABAAR γ2 • Gephyrin (scaffolding protein) • Synapsin (synapse)  Treatment • CNQX + AP5 (blocks AMPAR and NMDAR)  NEURONAL ACTIVITY • 4AP (blocks potassium channels)  NEURONAL ACTIVITY • Strychnine + SR95531 (blocks GlyR and GABAAR) 4AP blocks kv1 family of voltage activated K+ channels

7 RESULTS Increased excitatory activity
NEURONAL ACTIVITY - GABAAR AND GEPHYRIN CLUSTER SIZES RESULTS  Treatment • CNQX + AP5 (  neuronal activity) • 4AP (  neuronal activity) • St+ SR (  neuronal activity) GABAAR clusters Gephyrin clusters Increased excitatory activity decreases synaptic GABAAR and gephyrin cluster sizes

8  Depolarizing stimuli decrease GABAergic mIPSC amplitudes
NEURONAL ACTIVITY - GABAERGIC mIPSC AMPLITUDE RESULTS number of synaptic GABAAR Amplitude of mIPSC // 4AP induced hyperactivity  Patch clamp (whole cell) Recording mIPSC SR : antagonizes GABAAR What changes in GABAergic currents when neuronal activity is increased? Consistent with fig 1 Several studies: gaba mediated inhibition is reduced after repetitive stimulation protocols TTX + NBQX  Depolarizing stimuli decrease GABAergic mIPSC amplitudes

9  No dynamin-dependent internalization of GABAAR
NEURONAL ACTIVITY - GABAERGIC mIPSC AMPLITUDE RESULTS  GABAergic mIPSC Internalization of GABAAR?  Immunofluorescence : GABAAR γ2  Treatment : 4AP  Dynasore : membrane-permeable dynamin inhibitor  No dynamin-dependent internalization of GABAAR

10 RESULTS Depolarizing stimuli decrease GABAergic mIPSC amplitudes
NEURONAL ACTIVITY - GABAERGIC mIPSC AMPLITUDE RESULTS Total and surface GABAAR  Treatment : 4AP  Biotynilation (to isolate surface receptors)  Western blot GABAAR β3 No difference between total and surface receptors amounts  Quantification Depolarizing stimuli decrease GABAergic mIPSC amplitudes without internalization

11 RESULTS Lateral diffusion and local trapping
NEURONAL ACTIVITY - LATERAL DIFFUSION OF GABAAR RESULTS Lateral diffusion and local trapping number of synapse receptors GABAAR GABA Gephyrin Could neuronal activity alterate lateral diffusion?

12 NEURONAL ACTIVITY - LATERAL DIFFUSION OF GABAAR
RESULTS GABAAR Anti GABAAR γ2 Quantum dot Biotynilated Fab Streptavidin  Single particle tracking (SPT) / of GABAAR => QD GABAAR  FM4-64: active synapses Excitatory activity modifies GABAAR trapping at synapses GABAAR GABA Gephyrin Most have diffusive behavior Could neuronal activity alterate lateral diffusion? Extra synaptic Synaptic Extra synaptic  Treatment • CNQX + AP5 ( neuronal activity) • 4AP ( neuronal activity) • St+ SR ( neuronal activity)

13 NEURONAL ACTIVITY - LATERAL DIFFUSION OF GABAAR
RESULTS Surface exploration and trajectories of GABAAR Excitatory activity modifies GABAAR trapping at synapses GABAAR-QD FM4-64 Most have diffusive behavior Synaptic Extrasynaptic

14 NEURONAL ACTIVITY - LATERAL DIFFUSION OF GABAAR
RESULTS Synaptic dwell time (time a receptor remains in a synapse) and synaptic in-out transitions Dwell time Synaptic in-out transitions Excitatory activity modifies GABAAR trapping at synapses Synaptic stability of GABAAR is reduced when neuronal activity is increased

15 NEURONAL ACTIVITY - DIFFUSION PROPERTIES OF GABAAR
RESULTS Neuronal activity diffusion coefficients and confinement of GABAAR?  GABAAR-QD  FM4-64 (synaptic area) Diffusion coefficients Interquartile range (middle fifty, midspread) : measure of statistical dispersion. = difference between upper and lower quartiles. Used to characterize the data when extremeties might skew the data.  Increased neuronal activity increases diffusion coefficients of GABAAR

16 NEURONAL ACTIVITY - DIFFUSION PROPERTIES OF GABAAR
RESULTS Inhibitory or excitatory synapses?  Venus-tagged gephyrin construct Confinement size (surface of lateral diffusion)  Increased neuronal activity increases confinement size of GABAAR

17 NEURONAL ACTIVITY - DIFFUSION PROPERTIES OF GABAAR
RESULTS Neuronal activity changes in Ca2+ Extracellular or intracellular source of Ca2+?  EGTA : Ca2+ chelator  Ryanodine blocks ryanodine receptors  2APB blocks IP3 receptors  Ca2+ entry from the extracellular space is required for 4-AP dependent regulation of GABAAR dynamics

18 RESULTS CA2+ entry / NMDAR Diffusion dynamics of GABAAR
NEURONAL ACTIVITY - DIFFUSION PROPERTIES OF GABAAR RESULTS CA2+ /NMDA receptor reduces GABAergic transmission CA2+ entry / NMDAR Diffusion dynamics of GABAAR Increased diffusion coefficients of GABAAR-QD Increased confinement size of GABAAR-QD Intracellular Ca2+ Activation of NMDA receptors favors the escape of GABAAR from synapses by lateral diffusion

19 RESULTS Phosphatase calcineurin GABAAR diffusion dynamics
NEURONAL ACTIVITY - DIFFUSION PROPERTIES OF GABAAR RESULTS Ca2+ entry / NMDAR Long term depression at GABAergic synapses by activation of phosphatase calcineurin FT Calcineurin : Ca2+ sensitive phosphatase Phosphatase calcineurin GABAAR diffusion dynamics

20 NEURONAL ACTIVITY / DIFFUSION PROPERTIES OF GABAAR
RESULTS Phosphatase calcineurin GABAAR diffusion dynamics  Cyclosporin-A (CysA) : inhibitor of calcineurin NMDAR-Calcineurin signaling pathway is involved in the activity-dependent regulation of GABAAR diffusion dynamics

21 REGULATION OF TRAPPING AT SYNAPSE
CONCLUSIONS Synaptic accumulation and diffusion dynamics of GABAAR are controlled by excitatory activity Increased neuronal activity - ↘ GABAAR and gephyrin cluster sizes - ↘ mIPSC / GABAAR - ↗ diffusion coefficients (synaptic and extrasynaptic) and confinement size, mediated by Ca2+ entry / NMDAR and calcineurin REGULATION OF TRAPPING AT SYNAPSE

22 CONCLUSIONS Functional implications : During onset of LTP, depression of IPSC → enhance the ability of EPSC to elicit action potentials? Activity-dependent regulation of diffusion dynamics of GABAAR → LTP?

23 Thank you for your attention

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25  Depolarizing stimuli ↘ GABAergic mIPSC amplitudes
NEURONAL ACTIVITY / GABAERGIC mIPSC AMPLITUDE RESULTS  Patch clamp (whole cell) ↘ 78 % What changes in GABAergic currents when neuronal activity is increased? Consistent with fig 1 Several studies: gaba mediated inhibition is reduced after repetitive stimulation protocols Recovering control levels : 10 min // recovering levels of synaptic gephyrin (figure S3) No change in decay time = no channel kinetic changes / treatment  Depolarizing stimuli ↘ GABAergic mIPSC amplitudes

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