Presentation on theme: "BSL 1 Biosafety Training FHSc. Safety Office Health Sciences Center 1J11, ext. 24956 Print out the accompanying test and return (in handwriting) to 1J11."— Presentation transcript:
BSL 1 Biosafety Training FHSc. Safety Office Health Sciences Center 1J11, ext. 24956 Print out the accompanying test and return (in handwriting) to 1J11 HSC or fax to 905 528 8539
How to find us HSC 1J11 [PrintablePrintable
Information for undergrad courses You will be contacted only if you have failed your quiz, <75%. Your instructional assistant will be contacted at the same time. You will not be permitted to attend the biological labs of your course until you pass.
Biosafety in Canada: Standards Public Health Agency of Canada (PHAC) through the Laboratory Biosafety Guidelines Canadian Food Inspection Agency (CFIA) has Veterinary Standards for Animal Facilities Human Pathogens and Toxins Act C-11 Ministry of Environment Canada (MOE) for waste disposal
PHAC has Laboratory Biosafety Guidelines Sets the standards for work with biological agents such as bacteria, viruses, fungi parasites, prions and hazardous cell lines. The health risk associated with an organism will determine the protocols which must be followed to work with it.
Level of Hazard: BSL 1,2,3,4 Internationally and in Canada, there are four levels of biosafety, each associated with a type of lab and specific equipment. The personal protection required increases with the level of hazard. Labs, techniques and methods required increased safety with the level of hazard.
Risk evaluation to determine level of use Risk evaluation of an agent is part of the responsibility of the supervisor for the project and is reviewed by the University Presidential Biosafety Advisory Committee. All individuals working with an agent must comply with the level of risk.
Factors affecting risk agent in use eg. specific bacterium pathogenicity eg. degree of disease infectious dose eg. # organisms to cause disease mode of transmission eg. directly by blood or indirectly through the air
Factors affecting risk techniques in use host range eg. does it infect animals as well as humans effective treatment or prevention eg. is there a vaccine? Potential for aerosol creation eg. sonication, vortexing or homogenization
Factors affecting risk Volume of material eg > 10 liters Concentration of material Stability in the environment eg. will it survive on a benchtop? Use of re-combinant DNA eg. oncogenicity, replication ability, host range, ability to revert to wild type
Biosafety Level 1 (BSL 1) unlikely to cause disease in healthy workers or animals treatment / prevention is available Low individual risk Low community risk
Biosafety Level 2 (BSL 2) causes human or animal disease under appropriate use, is unlikely to cause death to healthy laboratory workers or animals treatment / prevention is available Moderate individual risk Limited community risk
Biosafety Level 3 (BSL 3) usually causes serious human or animal disease including death. Usually spreads by direct contact of blood or body fluids from one individual to another generally, no cure / prevention available High individual risk Moderate community risk
Biosafety Level 4 (BSL 4) human disease including death and may be readily transmitted through casual contact no treatment / prevention available High individual risk High community risk
Which level of organisms are being used in your lab ? Ask to see the biological equivalent of an MSDS for the agents you will be using. Or look up the information on the Internet site for the supplier of the product. Or reference a large database such as ATCC http://www.atcc.org/ orhttp://www.atcc.org/ PHAC http://www.phac-aspc.gc.ca/index- eng.php/http://www.phac-aspc.gc.ca/index- eng.php/
Basic Safety Practices - BSL 1 These requirements must be followed in all labs working with biological agents. Know and understand them.
Basic Safety Practices Longer hair is to be restrained so that it cannot come into contact with hands, specimens, containers or equipment. Oral pipetting of any substance is prohibited in any lab, use only mechanical fluid transfer devices.
Basic Safety Practices No eating or storage of food or drink in the labs…dispose of any food wrapping or containers BEFORE you enter the lab, including water bottles and gum. Keep personal items out of the working area of the lab.
Basic Safety Practices It is wise to report any increased susceptibility to infection to your supervisor or medical advisor. Ask the lab supervisor if you are unclear about any hazard or process. Dispose of all biological waste in the designated container only.
Waste disposal Biological waste is to be disposed of into the yellow or red bags in the biohazard labeled cardboard boxes Sharps (scalpel blades, razor blades, needles on syringes) must be disposed into a sharps container and then disposed as biological waste when ¾ full
Basic Safety Practices Open wounds, cuts, scratches and grazes should be covered with waterproof dressings BEFORE working with any biological material. Limit use of sharps and glass. DO NOT RECAP NEEDLES
Basic Safety Practices Minimize aerosol creation Label all biological material Where possible and appropriate, protect yourself by immunization esp Hep B when working with blood or body fluids.
Personal protection for BSL 1 Protective laboratory clothing must be worn in the lab but not worn out of the lab - closed toed footwear -wear lab coats at all times -goggles – if using contact lenses - if required by the protocol
Personal protection for BSL 1 Gloves…latex for water based materials …nitrile or vinyl for organics Change gloves when contaminated. Wash your hands frequently, particularly when leaving the laboratory.
Emergencies Staff must understand all hazards with which they work, including emergency response. - read and know the emergency and spill response protocol posted in your lab - report any spills or injuries to your supervisor -know the location of spill kits and first aid kits -know the location of eyewash and shower
Training personnel must receive training on biohazards and safe handling protocols - pass for biotraining is 75% - local lab training to be signed by trainee and trainer - retraining program includes annual update
Physical lab requirements for BSL 1 Physical requirements are a functional and well designed lab Doors to the laboratory must not be left open
Working requirements for BSL 1 Basic microbiology lab practices, keep the lab organized and clean Open bench is suitable Routine decontamination techniques to maintain sterility - contamination of cultures is a risk in an unorganized lab
Supervisor responsibilities for BSL 1 Access to the laboratory is restricted to authorized personnel ie. those who have been advised of the potential hazards, trained and approved by the supervisor
Supervisor responsibilities for BSL 1 Post the lab with a biohazard sign indicating the biosafety Level and organism(s) in use Contaminated materials or equipment leaving the lab must be appropriately decontaminated first
Supervisor responsibilities for BSL 1 All materials must be contained so that they will not be released to the environment. Disinfectants effective against the material in use, must be available at all times. Have available leak proof containers for transporting materials between labs.
Supervisor responsibilities for BSL 1 Maintain an effective insect and rodent control program. Investigate spills and accidents, keep records, use results in future training.
Supervisor responsibilities for BSL 1 Biological safety information, such as a manual, must be available - with procedures for the lab - it must be read and followed by all - it must be updated regularly
Supervisor responsibilities for BSL 1 Provide MSDSs or equivalent. Ensure compliance within lab. Any use of animals must comply with the CCAC and any training requirements of the CAF and follow the SOP.
Additional biosafety lab information 1) Bunsen burners 2) Ultraviolet light 3) Causes of contamination 4) airflow diagram of a biological cabinet 5) airflow diagram of a fume hood 6) airflow diagram of a clean air bench
Bunsen Burners Do not use combustibles or flammables in a biological cabinet – 70% recirculated will allow buildup of leaking gases Upward hot air flow disrupts downward laminar flow May create holes in the HEPA filters Splashes may occur (when using loops) and could cause contamination Substitute: Micro incinerator Alcohol burner
Ultra Violet Light UV lights are not recommended: they provide a false sense of security - not giving off UV light (just prior to the end life of the bulb) only disinfects the surface – could contact a layer of media protein covering the pathogen casts a shadow, leaving areas that remain unexposed to the UV wavelengths could cause burns / damage eyes – turn off when working in the room
Causes of Contamination 1) YOU! hands not clean, bacteria/spore under your nails gloves – not sterile from the box dirty lab coats lab coat sleeves should be taped place all materials (cleaned) required into hood BEFORE work 2) Incubator Avoid dip in line from CO2 tank Clean humidity source regularly Clean all surfaces after spill, including holes on trays If at ground level – tape bench paper onto floor
Class II Biological Safety Cabinet use with BSL 2 agents yes - personnel protection yes - product protection yes - environmental protection
Chemical Fume Hood do not use with biological agents yes - personnel protection no - product protection no - environmental protection
Clean Air Bench use only with BSL 1 no - personnel protection yes - product protection no - environmental protection
Information for McMaster staff/students Do not ship any samples, or take samples with you on a trip. Check with Mike McGuire to ensure it does not require Transportation of Dangerous Goods compliance File a Materials Transfer Agreement with Bertha Monrose before you share material with another institution
Information for McMaster staff/students Do not transform your own body cells. The immune protection you would normally have has now been bypassed Obtain a biosafety approval number from the Biosafety office, HSC 1J11 ext 24956 before importing material or working with biological material
Information for McMaster staff/students Cell lines can be BSL 1 or BSL 2. Check with your supplier or your supervisor. If you are isolating primary cell lines from ill humans or animals, they may be BSL 2 until they are well characterized. Check with the Biosafety office first.
Use of a biological cabinet Some labs may chose to use Class II biological cabinets to maintain sterility for their BSL 1 agents. The following slides cover information for the use of biological cabinets.
Before you begin Check to ensure you are using the right cabinet for your work. Check to see it has been certified in the last 12 months. Ensure the UV light is off anytime anyone is in the room. To start, let the cabinet run for 3 minutes to purge air.
Before you begin Use a disinfectant that will kill any organism in the cabinet Check to see that the window is in the correct position Do not place pens or paper inside the cabinet Never block the grilles Segregate clean items from dirty ones
Before you begin Wear a lab coat and tape the coat at the wrist Pull the gloves over the taped coat Sterilize the gloves with a 70% alcohol wash As you place items into the hood, spray them down with alcohol to sterilize
Before you begin Place sterile items on the left side Place items from the incubator at center back You may place a work pad soaked in disinfectant on the center front work area On the right side, place a waste bucket for fluids, one for sharps, one for solids
Working in the cabinet Keep lab doors closed and keep others out of the room Only one person working in the cabinet Use good aseptic technique Slow movements directly in or out Keep sterile and non-sterile items separated Do not use Bunsen burners in cabinet
Working in the cabinet Have posted spill protocol nearby No gum, food or drink See the two powerpoint presentations on Good Culture Practices and Misidentification and Contamination, to learn more about good practices and why they are important.
When you are finished Spray each item with a disinfectant as you take it out of the cabinet Fill the liquid waste to the max fill line to achieve appropriate concentration. Mark on the time when it can be disposed Seal the top of the solid waste bag, spray it, put it into the biohazard cardboard box Close the top of the sharps container, leave it in the hood
When you are finished Spray all interior surfaces with the disinfectant, remember to spray the plastic window facing you. If possible, leave the cabinet running. If not, let it purge for 3-4 minutes. If there is anything unusual, tell your supervisor and leave a note for the next person who will be using the hood.
Clean ----------------------- Dirty A new materials B materials from incubator C garbage D materials to go back into incubator E working surface F wet material solution (bleach) Suggested layout for working in a Class II biohood
Contact information For additional training videos, biosafety approval, import permits, reporting of spills or lab acquired infections, or general questions FHSc. Safety Office Health Sciences Center 1J11, ext. 24956 Carol Carte or Karin Cassidy