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Peter van t Erve Project Manager International Service Particle Measurement & Validation bv Erkenningsnummer 3445-00.

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Presentation on theme: "Peter van t Erve Project Manager International Service Particle Measurement & Validation bv Erkenningsnummer 3445-00."— Presentation transcript:

1 Peter van t Erve Project Manager International Service Particle Measurement & Validation bv Erkenningsnummer

2 Particle Measurement & Validation bv zIntroduction zBasic stuff zHEPA-filtration zTypes of cabinets zMaintenance zCriteria zDisinfection zHEPA-filter change zMiscellaneous Erkenningsnummer

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4 Basic stuff Basically a cabinet consists of : zThe Body : Stainless Steel Epoxy coated steel zPrefilter : Eff. 80 – 90% acc. EN 779 zHEPA-filters : Eff. 99,99% acc. EN zFan: To create the velocity zElectronics: To control the velocity and the alarm features zAccessories: on customer requirement

5 Basic stuff Pre-filters zCheap glassfiber filter-material, to protect the expensive HEPA- filters zTo be changed every year zLow cost exchange zLifetime of a HEPA: without Prefilter appr. 6 years with Prefilter : appr. 12 years Filter classification according EN 779 Filter typePre-filters & Fine-filters Filter classG1G2G3G4F5F6F6F7F7F8F9 Am %>6565<8080< Em %----40<6060<8080<9090<9595

6 Basic stuff H.E.P.A. filters zHEPA = High Efficiency Particulate Air zULPA = Ultra Low Particulate Air The purpose of a HEPA-filter is to catch small particles. Construction of a HEPA-filter: yFilter media (Glass fiber or similar) The media is pleated to a package of a certain size. The pleated media is glued together in a frame

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8 HEPA-Filtration: Efficiency zThe efficiency of a HEPA-filter according EN 12469: 99,99% when tested by means of a photometer particles 10 Particles

9 HEPA-Filtration Sieve operation of a HEPA

10 HEPA-Filtration Interception/impaction

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12 zProduct Protection zPersonal protection zProduct and personal protection Types of cabinets: General

13 zDownflow unit zCrossflow Types of cabinets: P roduct protection zLaminar Downflow

14 Class II A (Double exhaust) Class II B Class II A HEPA-Filter Pre-filter Types of cabinets: Biological safety cabinets

15 Inflow Recirculation Pressure plenum Downflow Exhaust Flow pattern 70% 30% Types of cabinets: Biological safety cabinets

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18 zCheck on Pre-filter zAirflow velocitys downflow & inflow zFilter efficiency test by means of a photometer or particle counter zCheck on alarmsettings Maintenance: General

19 zProduct Protection zPersonal protection zProduct and personal protection When do we need to change it? Turn over one edge of the pre-filter, If the down side is becoming grey, replace it. Take care of contaminated areas !! Maintenance : Control of Pre-filter

20 Maintenance: Control of air flow and velocity

21 Airflow velocity measurements Downflow Measurements have to be taken at - 8 positions minimum, - 50 to 100 mm above the top edge of the front aperture. Criteria: - All values have to be >0,25 and < 0,50m/s; - According manufacturers specifications; - No individual measurement should deviate more than 20% from the mean. Maintenance : Airflow measurements (EN 12469)

22 Inflow According Annex G you have to measure the velocity in the exhaust duct and calculate the volumetric rate of discharged exhaust air. With this air volume you can calculate the mean inflow velocity. In practice it is much more easier to measure the inflow velocity in the front aperture. Criteria: Mean inflow velocity > 0,40 m/s and, according manufacturers specifications. Maintenance : Airflow measurements (EN 12469) Airflow velocity measurements

23 Flow pattern Inflow: The direction of the inflow should be inward over the whole area of the front aperture. Downflow: The direction of the flow should be downwards without turbulence as a result of options or other obstructions in the working area. Maintenance : Airflow measurements (EN 12469) Tested using smoke

24 Procedure: Option 1. Filtertest with a Particle Counter and a test aërosol. Option 2. Efficiency test with a Photometer and a test aerosol. Criteria: Tested with a Photometer a local penetration should be not more than 0,01%. Related Standards: IES-RP-CC006.2 ISO/CD EUROVENT 4/8 Maintenance : Control of HEPA filter efficiency

25 Referential measurement ( µgr / L) 100% measurement Photometer Generating the test aerosol (DOP, Emery 3004, DEHS) Scanning probe Downflow / Crossflow unit Maintenance : Control of HEPA filter efficiency

26 Generating the test aerosol (DOP, Emery 3004, DEHS) 100% 100% measurement ( µgr / L) Photometer Probe Scanningprobe Maintenance : Control of HEPA filter efficiency

27 Photometer Generating the test aerosol (DOP, Emery 3004, DEHS) 100% measurement ( µgr / L) 100% Probe Scanningprobe Maintenance : Control of HEPA filter efficiency

28 Inflow zHigh Alarm yCreate a high alarm by increasing the voltage of the fan and check whether there is a high alarm. zLow Alarm yCreate a low alarm by de-creasing the voltage of the fan and check whether there is a high alarm. Downflow zHigh Alarm yCreate a high alarm by increasing the voltage of the fan and check whether there is a high alarm. zLow Alarm yCreate a low alarm by de-creasing the voltage of the fan and check whether there is a high alarm. Window Alarm (If applicable) Check whether there is an alarm if the window is not in a calibrated position. Other alarmpoints zCheck all other alarmpoints Maintenance: Alarm Settings (EN 12469)

29 Adjust alarmwindow to calculated alarmsettings Make your alarm window as big as possible Maintenance: Setting the alarms High alarm Determine High alarm (V av + 20%) Adjust alarmwindow to calculated alarmsettings First High alarm Adjust alarmwindow to calculated alarmsettings First High alarm Then Low alarm V av Adjust velocity Low alarm Determine Low alarm (V av - 20%)

30 Maintenance: Testpoints CA(B) / x and EF(B) / x cabinets A: Aerosol supply B: 100% measuring point (and test point for overall efficiency test 1st HEPA-filters on a CB and EFB) This point might also be on top of the cabinet for a CA(B) C: Scanning downflow HEPA-filter D: Scanning exhaust HEPA-filter (overall efficiency test on a CA(B)

31 There are three ways to connect a safety cabinet to a duct! 1.Direct connection! 2.Direct connection with a by-pass! 3.Connection via a thimble! Ducting: Ways of ducting

32 Both cabinet and the external ventilator have to be switched on at the same time, this is done by a potential free contact (optional accessory) coming from the cabinet! The anti blow-back-valve assures that no contaminated air can travel back into the cabinet! The room pressure will fluctuate depending if the cabinet is switched on or off. However it is possible to have the air inflow adjusted to the amount of air that is extracted from the room! Advantage: Higher level of safety! Externalfan Anti Blow Back Valve Airsupply Ducting: Direct connection

33 Externalfan Anti Blow Back Valve Airsupply By-pass valve The external ventilator works 24hrs a day! When the cabinet is switched on the by-pass valve in the duct will open in order to extract the exhausted air from the cabinet. When the cabinet is switched off the valve will close and will extract the same amount of air from the room! The valve is also controlled by a potential free contact coming from the cabinet. The anti blow-back-valve assures that no contaminated air can travel back into the cabinet! Advantage: Higher level of safety and the room pressure will be constant at all time! Ducting: Bypass connection

34 Effects on the roompressure: When the cabinet is switched on the exhausted air will be extracted from the cabinet! When the cabinet is switched off the exhausted air will be extracted from the room! Advantage: No adjustments have to be made to the cabinet and the pressure in the room will be constant! Externalfan Airsupply Ducting: Via a Thimble

35 There are three possibilities: z There is no extra fan in the exhaust system. z There is an extra fan in the exhaust system, but there is no link between the status (Day- or Stand-by position) of the cabinet and extra exhaust fan. z There is an extra fan in the exhaust system, with a link between the status (Day- or Stand-by position) of the cabinet and extra exhaust fan. Adjusting the alarms can be done by increasing or decreasing the downflow and inflow air velocity by means of the fan of the cabinet. What will be the effect on the alarm settings if the cabinet is connected on an exhaust duct work?

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37 z Why desinfection: - Microbiological Safety Cabinet is a Biohazard. - All parts in the cabinet are a possible risk for both personnel and environment when opened. z When desinfection: - If repair needs to be done inside the cabinet. z With what can we desinfect a cabinet Air mixed with fresh air and product HEPA-filtered air mixed with product Contaminated air HEPA-filtered air - That depends on the product which was handled in the cabinet, responsible for desinfection is the head of the laboratory. Disinfection

38 Air mixed with fresh air and product HEPA-filtered air mixed with product Contaminated air HEPA-filtered air z Clean Air techniek offers a so called FAS 2000 which is using Formalin for desinfection and Ammonia to neutralize the Formalin afterwards. FAS 2000 Disinfection Using Formalin

39 z Depending on the size (volume) of the cabinet, Formalin is put in the can with the red cap and Ammonia is put in the can with the blue cap. FAS 2000 Disinfection Using Formalin

40 z In order to prevent Formaldehyde of getting into the laboratory, the cabinet has to be sealed off. z You can do this by sealing the cabinet with a plastic bag. Make sure that the bag is sealed to the floor. Disinfection Using Formalin

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42 z First HEPA-filter on a Class IIB cabinet: HEPA-filter change 1st HEPA

43 z Take of the pressurebar. (On older models you have to cut the silicon along the outer edge of the filter.) HEPA-filter change 1st HEPA

44 z Seal the opening of the HEPA-filter in order to prevent parts to come out of the HEPA-filter HEPA-filter change 1st HEPA

45 z Replace the HEPA by a new one and put the pressure bar back in its place. HEPA-filter change 1st HEPA

46 z Take of the backpanel zDisconnect the fan and take it out HEPA-filter change Downflow HEPA

47 z Unloosen the pressure bars of the downflow plenum and the exhaust HEPA and take them out. z Lift the pressure plenum and take it out. z Take out the HEPA-filter. Pressure bars Plenum HEPA-filter HEPA-filter change Downflow HEPA

48 z Put in a new HEPA-filter with the seal downwards and put everything back in its place. HEPA-filter change Downflow HEPA

49 z Put in a new HEPA-filter with an extra seal on one end. z Put everything back in its place z Lift the exhaustduct z Unloosen the pressurebars and take them out z Lift the pressure plenum and take it out z Take out the HEPA-filter HEPA-filter change Exhaust HEPA

50 Criteria

51 Criteria Cabinet type EF / x (EN 12469) - HEPA-filter efficiency downflow > 99,99% - HEPA-filter efficiency exhaust > 99,99% - Vaverage downflow velocity: 0,35 m/s. -Alarm for downflow velocity: Vaverage +/- 20% as where all individual measurements should be > 0,25m/s and < 0,50m/s. - Vaverage inflow velocity : > 0,30 m/s at the top of the working opening. - Alarm for inflow velocity: < 0,28 m/s at the top of the working opening.

52 - HEPA-filter efficiency downflow > 99,99% - HEPA-filter efficiency exhaust > 99,99% - Vaverage downflow velocity: 0,35 m/s. -Alarm for downflow velocity: Vaverage +/- 20% as where all individual measurements should be > 0,25m/s and > 0,50m/s. - Vaverage inflow velocity : > 0,30 m/s at the top of the working opening. - Alarm for inflow velocity: < 0,28 m/s at the top of the working opening. - HEPA-filter efficiency 1st HEPA > 99,99% Criteria Cabinet type EFB/ x (EN 12469)

53 Criteria CLF x and DLF x - According Federal Standard 209B - HEPA-filter efficiency > 99,99% - Vaverage downflow velocity: 0,35 m/s. - Alarm : Vaverage - 20% and Vaverage +20% if applicable. - Vaverage horizontal velocity: 0,45 m/s. zDownflow unit zCrossflow

54 Criteria Standards

55 Video

56 Test equipment

57 VACUUM UP STREAM DOWN STREAM FILTER 3 WAY VALVE LIGHT STOP CONE OF LIGHT CONE OF DARKNESS LIGHT SOURCE PHOTO- MULTIPLIER TUBE Test equipment Photometer

58 Test equipment Aerosolgenerator, generating a testaerosol DOP, EMERY 3004 or DEHS SIGHT GLASS AEROSOL RESERVOIR PRESSURE REGULATOR ADJUSTING KNOB NITROGEN INLET FILTER ORIFICE AND VALVE HEATER BLOCK


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