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Influence of endothelial cell seeding on platelet deposition and patency in small- diameter Dacron arterial grafts Brent T. Allen, M.D., Julie A. Long, M.D., Richard E. Clark, M.D., Gregorio A. Sicard, M.D., Kevin T. Hopkins, B.S., Michael J. Welch, Ph.D. Journal of Vascular Surgery Volume 1, Issue 1, Pages (January 1984) DOI: / (84) Copyright © 1984 Mosby, Inc. Terms and Conditions
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Fig. 1 Scanning electron micrograph of luminal surface of a nondistended external jugular vein after endothelial cell harvesting. All but one (arrow) of the endothelial cells were removed, which left a surface composed of collagen and smooth muscle. (×640.) Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Mosby, Inc. Terms and Conditions
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Fig. 2 Scanning electron micrograph of luminal surface of a freshly seeded Dacron graft. A cluster of endothelial cells (arrow) is seen among Dacron fibers, red cells, platelets, and fibrin. (×640.) Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Mosby, Inc. Terms and Conditions
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Fig. 3 Serial indium 111—labeled platelet scintigrams from femoral region of a dog studied at 24 hours, 2 weeks, 1 month, and 2 months postoperatively. Platelet deposition on control grafts (large arrows) decreased from 24 hours to 2 weeks but markedly increased after withdrawal of aspirin therapy. Platelet accumulation on endothelial cell—seeded grafts (small arrows) appeared less than that on controls by 2 weeks and continued to decrease after withdrawal of aspirin therapy. Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Mosby, Inc. Terms and Conditions
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Fig. 4 There was no significant difference between carotid and femoral regions in %InExe for similar grafts (control or seeded). Analysis of %InExe data for each type of graft was performed after results from carotid and femoral regions were combined. Statistical comparisons (p values) between control and seeded grafts at each time interval are indicated above values for n. Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Mosby, Inc. Terms and Conditions
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Fig. 5 Patent control graft (A) explanted at 1 month from right carotid artery (RC) demonstrates white areas of neointimal pannus ingrowth from each anastomosis. Neointima was not complete, however, as demonstrated by a scanning electron micrograph (B) taken from dark midportion (squared area in A) of a control graft, which revealed a surface composed of fibrin red cells and platelet aggregates. An endothelial cell—seeded graft (C) explanted at 3 months from left carotid artery (LC) exhibits a complete neointimal lining. A smooth layer of endothelial cells is seen in a scanning electron micrograph (D) taken from squared area of seeded graft in C. (B and D, Original magnification ×1250.) Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Mosby, Inc. Terms and Conditions
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Fig. 6 A control graft that thrombosed between 2 and 3 months. Blood flow is toward RF. Areas of neointimal pannus ingrowth are indicated by small arrows. Graft occlusion appeared to result from subintimal thickening at distal anastomosis (large arrow). Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Mosby, Inc. Terms and Conditions
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