2Sharps Safety http://web.mit.edu/cohengroup/safety/sharps.pdf A high degree of precaution must always be taken with any sharp items used in the laboratory, including needles and syringes, glass slides and cover slips, Pasteur pipettes, capillary tubes, as well as broken glass, and scalpels, blades, and knives. Sharps must be disposed of in an approved sharps container. Sharps containers may never be placed in the normal waste stream or used for any purpose other than sharps disposal.Sharps containers have to be:Puncture ResistantClearly markedWithin easy reach of the work stationFilled to no more than 3/4 capacitySealed (i.e. capped or taped) prior to transport
3Sharps Safety CHEMICALLY CONTAMINATED SHARPS: Sharps contaminated with hazardous materials must be placed in a puncture proof container and sealed with a screw-on cap. The container must be labeled as hazardous waste, with the associated chemicals identified. Contact Phil Dennicort in the Chemical Stock Room (x 2338) for disposal.In case you poke yourself with sharps:Squeeze out blood;If necessary, seek medical attention immediately (know what was the contaminants of the sharps/what was in the needle).
4Safety Topic – Chemical Hood http://web. mit General purpose: prevent exposure to toxic, irritating, or noxious chemical vapors and gases. A face velocity of 100 feet per minute (fpm) provides efficient vapor capture while reducing hood turbulence.Baffles -- keep the airflow uniform across the hood opening, thus eliminating dead spots and optimizing capture efficiency.Sash --Airflow across the hood can be adjusted by sash height to the point where capture of contaminants is maximized.Airfoil -- Preventing the creation of turbulent eddies that can carry vapors out of the hood. The space below the bottom airfoil provides source of room air for the hood to exhaust when the sash is fully closed.Exhaust plenum -- An important engineering feature, the exhaust plenum helps to distribute airflow evenly across the hood face.Face -- The imaginary plane running between the bottom of the sash to the work surface. Hood face velocity is measured across this plane.
5Common Chemical Hood Misconception Myth - When working with highly hazardous materials, the higher the face velocity the betterWhile it is important to have a face velocity between 100 and 125 fpm, velocities higher than this are actually harmful. When face velocity exceeds 125 fpm eddy currents are created which allow contaminants to be drawn out of the hood, increasing worker exposures.Myth - A chemical hood can be used for storage of volatile, flammable, or odiferous materials when an appropriate storage cabinet is not available.Hoods are not designed for permanent chemical storage. Each item placed on the work surface interferes with the directional airflow, causing turbulence and eddy currents that allow contaminants to be drawn out of the hood.Myth - The airfoil on the front of a hood is of minor importance. It can safely be removed if it interferes with my experimental apparatus.Airfoils are critical to efficient operation of a chemical hood. With the sash open an airfoil smoothes flow over the hood edges. Without an airfoil eddy currents form, causing contaminates to be drawn out of the hood. With the sash closed, the opening beneath the bottom airfoil provides for a source of exhaust air.
6Safe Hood Operating Procedure Constant volume hood – the volume of air exhausted is constant, regardless ofsash height.Proper positioning of the sash is vital to maintaining the optimum face velocity (100 or 125 fpm).Too high: lowers face velocity, allowing contaminants to escape from the hoodToo low: results in very high face velocity, excessive turbulence and loss of containmentConfirm that the hood is operational: switch ‘on’, airflow gauge or ‘flow check ribbon’ hood test data and optimum sash height - yellow label affixed to the hood faceMaintain operations at least 6" inside the hood face.Lower sash to optimum height: maximized airflow without turbulence (17” in accordance to the rules in Colgate UniversityKeep head out of hoodKeep hood storage to an absolute minimumMinimize foot traffic around the chemical hoodUse extreme caution with ignitionReplace hood components prior to use
7Protect Your Eyes http://www. sterlingschools Appropriate eye protection must be worn at all times!
8Wear appropriate protective clothing Your clothing should cover your legs to the knees – shorts are not appropriate for the laboratoryLab aprons can be used to protect good clothingLoose clothing should not be worn because it may dip into chemicals or fall into a flame and catch fire
9Wear shoes that cover your feet. Sandals and open-toed shoes do not protect your feet from broken glass that is frequently found in the labAlso, leather shoes protect your feet from chemical spills – canvas shoes do not.
10Do not apply cosmetics, eat, or drink in the lab. These activities are ways by which you can accidentally ingest harmful chemicals
20Do not smell any chemicals directly! If absoluteley necessary to smell, use your hand to fan the vapor to your nose.
21Do not pipet solutions by mouth! Use a rubber suction bulb or other device to fill a pipet.
22Wash your hands with soap and water before leaving. This rule applies even if you have been wearing gloves!
23Know the hazards of the materials being used. Read and reread labels carefully to make sure that you are using the right chemical.Know how to interpret data from a MSDS.
24Tie Back Loose HairDangling hair can fall into the Bunsen burner and catch fire or can fall into a chemical solutionP.S. Burning hair REALLY STINKS!
25Know the safety equipment Eye wash fountainSafety showerFire extinguisherEmergency exits
26Know how to use the safety equipment Eye wash FountainSafety ShowerFire extinguisherEmergency Exit
27Know how to respond to an emergency Clarkson UniversityEmergensy Number (after working hours)Campus SafetyFire DepHospital /3304/5720Police /2122Rescue Squad(numbers are located near the each lab exit door)
28Never remove chemicals from the laboratory This guy put chemicals in his locker!
29Don’t work alone in the lab In case of a problem, you may need another person to prevent injury or even save your life!
30Remember that the lab is a place for serious work! Careless behavior may endanger yourself and others and will not be tolerated!
31Demonstrate safe behavior Obey all safety instructions.Clean up spills immediately; IF you know how. If you are uncertain how to clean up a spill or if a large spill occurs, notify persons in accordance to the emergency procedure.
32Demonstrate safe behavior Before leaving the lab:
33Demonstrate safe behavior Before leaving the lab:Return equipment and chemicals to their proper places
34Demonstrate safe behavior Before leaving the lab:Return equipment and chemicals to their proper placesBe sure to replace the lids to all containers
35Demonstrate safe behavior Before leaving the lab:Return equipment and chemicals to their proper placesBe sure to replace the lids to all containersClean up your work area
36Know how to dispose of waste Dispose of all waste materials according to your instructional? ? ? ?
37Report any accidents or unsafe conditions immediately!