1. Fertility and Sterility
Analysis of apoptotic cell death, Bcl-2, and p53 protein expression in freshly fixed and cryopreserved ovarian tissue after exposure to warm ischemia 
Mahmoud R. Hussein, M.Sc.Path, M.D., Ph.D., Mohamed A. Bedaiwy, M.D., Tommaso Falcone, M.D. 
Fertility and Sterility 
Volume 85, Pages (April 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Apoptosis in healthy (A–D), and atretic (E–H) ovarian follicles and HL60 promylocytic leukemia cells (I–L, control). A, B, E, F, I, and J: hematoxylin-eosin stained; C, D, G, H, K, and L: TUNEL stained. Black arrow = TUNEL signal–positive cell; red arrow = TUNEL signal–negative cell.
Hussein. p53 and Bcl-2 proteins in cryopreserved ovaries. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Bcl-2 protein expression (cytolasmic) in the primary (A, 400×), secondary (B, 400×), graafian (C, 400×), and atretic (D, 100×) follicles.
Hussein. p53 and Bcl-2 proteins in cryopreserved ovaries. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Bcl-2 protein expression in the different types of the ovarian follicles (freshly fixed and cryopreserved). The data are presented as a function of time as a continuous variable. The letters A, B, C, and D correspond to 0, 10, 20, and 30 min, respectively. Dark blue = primordial follicles; pink = primary follicles; yellow = secondary follicles; light blue = atretic follicles.
Hussein. p53 and Bcl-2 proteins in cryopreserved ovaries. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Lack of p53 protein expression (cytoplasmic) in the primary (A), secondary (B), graafian (C), and atretic (D) follicles (400×).
Hussein. p53 and Bcl-2 proteins in cryopreserved ovaries. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

6. FIGURE 5
Apoptotic index (AI) and Bcl-2 protein expression in the healthy follicles (freshly fixed and cryopreserved ovarian tissues). The data are presented as a function of time as a continuous variable. The letters A, B, C, and D correspond to 0, 10, 20, and 30 min, respectively. Dark blue = AI in freshly fixed tissue; pink = AI in cryopreserved-thawed tissue; yellow = Bcl-2 in freshly fixed–thawed tissue; light blue = Bcl-2 in cryopreserved-thawed tissue.
Hussein. p53 and Bcl-2 proteins in cryopreserved ovaries. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

7. FIGURE 6
Apoptotic index (AI) and Bcl-2 protein expression in the atretic follicles (freshly fixed and cryopreserved ovarian tissues). The data are presented as a function of time as a continuous variable. The letters A, B, C, and D correspond to 0, 10, 20, and 30 min, respectively. Dark blue = AI in freshly fixed tissue; pink = AI in cryopreserved-thawed tissue; yellow = Bcl-2 in freshly fixed–thawed tissue; light blue = Bcl-2 in cryopreserved-thawed tissue.
Hussein. p53 and Bcl-2 proteins in cryopreserved ovaries. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions