1. 1 Next Generation Sequencing Data Processing How to use the Virtual Machine (VM) Andreas Gisel Institute for Biomedical Technologies CNR Bari - Italy

2. 2 Virtual Machine (VM) A virtual machine is a tightly isolated software container that can run its own operating systems and applications as if it were a physical computer. A virtual machine behaves exactly like a physical computer and contains it own virtual (ie, software-based) CPU, RAM hard disk and network interface card (NIC). Benefits In general, VMware virtual machines possess four key characteristics that benefit the user: C ompatibility: Virtual machines are compatible with all standard x86 computer I solation: Virtual machines are isolated from each other as if physically separated E ncapsulation: Virtual machines encapsulate a complete computing environment H ardware independency: Virtual machines run independently of the underlying hardware

3. 3 Installing the VM Player

4. 4 Starting the VM

5. 5 set virtul RAM not bigger than half of your real RAM Starting the VM about 15min.

6. 6 Starting the VM user: embnet password: embnet2012

7. 7 Starting the VM Ubuntu Manual short description of the Virtual Machine

8. 8 Setting up the VM In case you have no connectivity set up a new wired connection adding ONLY a name and save

9. 9 Setting up the VM Connect your USB stick Connect USB stick unmount from host machine

10. 10 Setting up the VM Get started for the Tutorial

11. 11 Setting up the VM Stop the Virtual Machine

12. 12 THANKS and enjoy your Virtual Machine