1. TIE2hi/VEGFAhi TEMs are present in the TMEM
TIE2hi/VEGFAhi TEMs are present in the TMEM. A, immunofluorescence imaging of the TMEM. Macrophages (CD68; gray), blood vessels (CD31; green), tumor cells (MENA; red), and DAPI (blue).
TIE2hi/VEGFAhi TEMs are present in the TMEM. A, immunofluorescence imaging of the TMEM. Macrophages (CD68; gray), blood vessels (CD31; green), tumor cells (MENA; red), and DAPI (blue). TMEM in white box (right). Scale bar, 15 μm. B, immunofluorescence imaging of VEGFAhi macrophages in the TMEM in sequential sections. Scale bar, 10 μm. Tumor cell, spotted line; macrophages, solid line; and blood vessels, dashed line. Left, macrophages (CD68; gray), tumor cells (MENA; red), blood vessels (CD31; green), and DAPI (blue). Sequential section (center), VEGFA (red), TIE2 (gray), blood vessels (CD31; green), and DAPI (blue). Schematic representation (right) of protein expression in the TMEM; tumor cells with MENAhi (red); endothelial cells CD31 (green); and macrophages CD68, VEGFAhi, and TIE2hi (gray). M, macrophage; TC, tumor cell; and EC, endothelial cell. C, immunofluorescence images of TIE2, VEGFA, and CD31. Yellow lines, regions of intensity profiling of VEGFA intensity for CD31 (EC), macrophage (M), and tumor cells (TC). Scale bar, 25 μm. D, fluorescence intensity profile of VEGFA from C of macrophage, endothelial cell, and tumor cells. E, immunofluorescence imaging of sequential PyMT tumor sections for TEM markers. VEGFAhi TMEM macrophages express F4/80, MRC1, CD11b, and CD68 as indicated by a yellow arrowhead in sequential sections. CD31+ endothelium is indicated by a white arrowhead. F, VEGFAhi TMEM macrophages express TIE2, MRC1, and CD68 but not CD11c as indicated by a yellow arrowhead. CD31+/TIE2+ endothelium is indicated by a white arrowhead. Scale bar, 25 μm.
Allison S. Harney et al. Cancer Discovery 2015;5:
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