Presentation is loading. Please wait.

Presentation is loading. Please wait.

Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies  H. Pereira, S.G. Caridade,

Published byIlhami Eriş Modified over 5 years ago

Similar presentations

Presentation on theme: "Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies  H. Pereira, S.G. Caridade,"— Presentation transcript:

1 Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies  H. Pereira, S.G. Caridade, A.M. Frias, J. Silva-Correia, D.R. Pereira, I.F. Cengiz, J.F. Mano, J.M. Oliveira, J. Espregueira-Mendes, R.L. Reis  Osteoarthritis and Cartilage  Volume 22, Issue 9, Pages (September 2014) DOI: /j.joca Copyright © Terms and Conditions

2 Fig. 1 Lateral meniscus obtained from live donor that undergone a TKA for unicompartmental OA (A), meniscus division in anterior, mid body and posterior segments (B), and harvesting of 4 mm cylinders (C). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © Terms and Conditions

3 Fig. 2 DMA of fresh lateral and medial meniscus studied at several frequencies (mean with 95% confidence interval (CI), n = 108 samples from 15 donors): segmental evaluation of E′ values for lateral (A) and medial menisci (B); segmental evaluation of E′′ values for lateral (C), and medial (D) menisci; and segmental evaluation of tan δ for lateral (E) and medial (F) menisci. Numeric data are presented on Table 2s of Supplementary data. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © Terms and Conditions

4 Fig. 3 3D reconstruction from micro-CT images of freeze-dried lateral meniscus of left knee (A); 2D micro-CT images of a part of freeze-dried medial meniscus of left knee (B, C), change of porosity (%) across the menisci from the bottom part (tibial surface) to top part (femoral surface) (D). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © Terms and Conditions

5 Fig. 4 Microscopy image of the HMC's in culture after isolation using an enzymatic digestion method. It is possible to observe meniscus cells depicting rounded and fusiform-like morphologies (A). The mean value (n = 5 patients) of percentage of positive cells for markers CD73, CD90, and CD105, is 97.2 (95.6–98.9)%, and for the marker CD44 the mean percentage value is 96.7 (95.1–98.2)%. The same densities were also analyzed for the expression of CD31, CD34 and CD45, and the obtained mean percentage values are 2.1 (1.8–2.4)%, 3.1 (2.9–3.3)% and 0.2 (0.1–0.2)%, respectively. Besides, two different passages (P2, P7) were analyzed (n = 2); and it was found that the analyzed markers and their percentages of expression were maintained. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © Terms and Conditions

Download ppt "Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies  H. Pereira, S.G. Caridade,"

Similar presentations

Efficacy of ultrasound therapy for the management of knee osteoarthritis: a systematic review with meta-analysis A. Loyola-Sánchez, J. Richardson, N.J.

Efficacy of ultrasound therapy for the management of knee osteoarthritis: a systematic review with meta-analysis A. Loyola-Sánchez, J. Richardson, N.J.
B. J. Kim, D. -W. Kim, S. H. Kim, J. H. Cho, H. J. Lee, D. Y. Park, S

B. J. Kim, D. -W. Kim, S. H. Kim, J. H. Cho, H. J. Lee, D. Y. Park, S
Advances in combining gene therapy with cell and tissue engineering-based approaches to enhance healing of the meniscus  M. Cucchiarini, A.L. McNulty,

Advances in combining gene therapy with cell and tissue engineering-based approaches to enhance healing of the meniscus  M. Cucchiarini, A.L. McNulty,
X. I. Gu, P. E. Palacio-Mancheno, D. J. Leong, Y. A. Borisov, E

X. I. Gu, P. E. Palacio-Mancheno, D. J. Leong, Y. A. Borisov, E
Effect of interval-training exercise on subchondral bone in a chemically-induced osteoarthritis model  A. Boudenot, N. Presle, R. Uzbekov, H. Toumi, S.

Effect of interval-training exercise on subchondral bone in a chemically-induced osteoarthritis model  A. Boudenot, N. Presle, R. Uzbekov, H. Toumi, S.
T1ρ relaxation time of the meniscus and its relationship with T1ρ of adjacent cartilage in knees with acute ACL injuries at 3T  R.I. Bolbos, Ph.D., T.M.

T1ρ relaxation time of the meniscus and its relationship with T1ρ of adjacent cartilage in knees with acute ACL injuries at 3T  R.I. Bolbos, Ph.D., T.M.
J. K. Meckes, B. Caramés, M. Olmer, W. B. Kiosses, S. P. Grogan, M. K

J. K. Meckes, B. Caramés, M. Olmer, W. B. Kiosses, S. P. Grogan, M. K
Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis  H. Iijima,

Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis  H. Iijima,
Knee osteoarthritis patients with severe nocturnal pain have altered proximal tibial subchondral bone mineral density  W.D. Burnett, S.A. Kontulainen,

Knee osteoarthritis patients with severe nocturnal pain have altered proximal tibial subchondral bone mineral density  W.D. Burnett, S.A. Kontulainen,
Two year longitudinal change and test–retest-precision of knee cartilage morphology in a pilot study for the osteoarthritis initiative  F. Eckstein, M.D.,

Two year longitudinal change and test–retest-precision of knee cartilage morphology in a pilot study for the osteoarthritis initiative  F. Eckstein, M.D.,
A. Hennerbichler, M. D. , F. T. Moutos, M. S. , D. Hennerbichler, M. D

A. Hennerbichler, M. D. , F. T. Moutos, M. S. , D. Hennerbichler, M. D
The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in sheep and goats  C.B. Little, M.M. Smith, M.A.

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in sheep and goats  C.B. Little, M.M. Smith, M.A.
Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis  H. Iijima,

Subchondral plate porosity colocalizes with the point of mechanical load during ambulation in a rat knee model of post-traumatic osteoarthritis  H. Iijima,
Mice null for the alternatively spliced EDA segment of fibronectin exhibit reduced knee inflammation after tibial compression injury  J. Peters, J. Fong,

Mice null for the alternatively spliced EDA segment of fibronectin exhibit reduced knee inflammation after tibial compression injury  J. Peters, J. Fong,
Determining collagen distribution in articular cartilage using contrast-enhanced micro- computed tomography  H.J. Nieminen, T. Ylitalo, S. Karhula, J.-P.

Determining collagen distribution in articular cartilage using contrast-enhanced micro- computed tomography  H.J. Nieminen, T. Ylitalo, S. Karhula, J.-P.
Synovial changes detected by ultrasound in people with knee osteoarthritis – a meta- analysis of observational studies  A. Sarmanova, M. Hall, J. Moses,

Synovial changes detected by ultrasound in people with knee osteoarthritis – a meta- analysis of observational studies  A. Sarmanova, M. Hall, J. Moses,
C. Pauli, S. P. Grogan, S. Patil, S. Otsuki, A. Hasegawa, J. Koziol, M

C. Pauli, S. P. Grogan, S. Patil, S. Otsuki, A. Hasegawa, J. Koziol, M
Osteoarthritic changes in vervet monkey knees correlate with meniscus degradation and increased matrix metalloproteinase and cytokine secretion  A.V.

Osteoarthritic changes in vervet monkey knees correlate with meniscus degradation and increased matrix metalloproteinase and cytokine secretion  A.V.
Bone marrow lesions, subchondral bone cysts and subchondral bone attrition are associated with histological synovitis in patients with end-stage knee.

Bone marrow lesions, subchondral bone cysts and subchondral bone attrition are associated with histological synovitis in patients with end-stage knee.
The relationship between meniscal pathology and osteoarthritis depends on the type of meniscal damage visible on magnetic resonance images: data from.

The relationship between meniscal pathology and osteoarthritis depends on the type of meniscal damage visible on magnetic resonance images: data from.

Similar presentations

Ads by Google