1. Volume 10, Issue 1, Pages 27-39 (July 2009)
Histone Methylation Regulator PTIP Is Required for PPARγ and C/EBPα Expression and Adipogenesis 
Young-Wook Cho, SunHwa Hong, Qihuang Jin, Lifeng Wang, Ji-Eun Lee, Oksana Gavrilova, Kai Ge 
Cell Metabolism 
Volume 10, Issue 1, Pages (July 2009)
DOI: /j.cmet
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2. Figure 1 PTIP Is Required for PPARγ Expression in MEFs
(A and B) Immortalized PTIPflox/flox MEFs were infected with retrovirus MSCVpuro expressing Cre or vector (Vec) alone. Total RNA was extracted for confirmation of putative PTIP-regulated genes identified in microarray analysis (Table S1). Shown are northern blot (A) and qRT-PCR (B). PPARγ2 mRNA level is below detection limit in qRT-PCR. AK5 and Flt1 stand for adenylate kinase 5 and FMS-like tyrosine kinase 1, respectively. 36B4 is the loading control.
(C) Ectopic PTIP can rescue PPARγ expression in PTIP-deficient cells. PTIPflox/flox MEFs were sequentially infected with retroviruses MSCVhygro, expressing Cre, and MSCVpuro, expressing FLAG-tagged PTIP (F-PTIP). PPARγ expression was analyzed by qRT-PCR.
(D) ChIP assay of binding of the PTIP-associated HMT complex subunits to PPARγ1 and PPARγ2 promoters in PTIPflox/flox MEFs. Top panel, schematic of mouse PPARγ1 and PPARγ2 promoters. The black bars indicate locations of Taqman probes used for PCR quantitation of ChIP assays. Pol II, RNA polymerase II.
(E) ChIP assay of H3K4me3 on PPARγ1 and PPARγ2 promoters in PTIPflox/flox MEFs. me3, H3K4me3.
(F) ChIP assay of H3K4me3 and H3K27me3 on PPARγ1 promoter in PTIPflox/flox MEFs. All results are representative of 2–4 independent experiments. Quantitative PCR data in all figures are presented as means ± SD.
Cell Metabolism  , 27-39DOI: ( /j.cmet )
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3. Figure 2
PTIP Is Required for Adipogenesis of Primary MEFs and Preadipocytes
(A–C) PTIP is required for adipogenesis of primary MEFs. Primary PTIPflox/flox MEFs were infected with adenovirus expressing Cre (Ad-Cre) or control virus expressing GFP alone, followed by adipogenesis assay. To determine Ad-Cre-mediated gene deletion efficiency, PTIP expression was analyzed by qRT-PCR before induction of adipogenesis (A). Morphological differentiation under the phase-contrast microscope is shown with oil red O staining (B). qRT-PCR analysis of gene expression after adipogenesis is also shown (C).
(D–F) PTIP is required for differentiation of 3T3-L1 white preadipocyte cell line. 3T3-L1 cells were infected with pSUPER.retro-based retroviral shRNA construct targeting PTIP or vector alone. After puromycin selection, cells were induced to undergo adipogenesis for 8 days. Western blot of PTIP expression before induction of differentiation is shown (D). The asterisk indicates a nonspecific band. Also shown are morphological differentiation (E) and qRT-PCR analysis of gene expression 8 days after induction of differentiation (F).
(G–L) PTIP is required for differentiation of primary white (G–I) and brown (J–L) preadipocytes. Primary white and brown preadipocytes isolated from PTIPflox/flox mice were infected with Ad-Cre or control virus expressing GFP, followed by adipogenesis assay. qRT-PCR analysis of Cre-mediated PTIP gene deletion before differentiation is shown in (G) and (J). Morphological differentiation is shown in (H) and (K) (top panels, stained dishes; lower panels, representative fields under the microscope). After differentiation, total RNA was isolated and subjected to qRT-PCR analysis of expression of genes common to white and brown adipocytes (PPARγ, PPARγ1, PPARγ2, C/EBPα, aP2) and genes specific for brown adipocytes (PRDM16, cidea, eva1, UCP1, PGC-1α), as shown in (I) and (L) (Seale et al., 2007). All results are representative of 2–4 independent experiments. Quantitative PCR data in all figures are presented as means ± SD.
Cell Metabolism  , 27-39DOI: ( /j.cmet )
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4. Figure 3
Both PTIP-Deficient and C/EBPα-Deficient MEFs Show Defects in PPARγ-Stimulated Adipogenesis
(A–D) PTIP-deficient MEFs show significant defects in PPARγ-stimulated adipogenesis. Immortalized PTIPflox/flox MEFs expressing ectopic PPARγ were infected with MSCVpuro expressing Cre or vector alone, followed by adipogenesis assay in the presence or absence of synthetic PPARγ ligand Rosi. Shown are: schematic of the PPARγ-stimulated adipogenesis assay (A), western blot of endogenous PTIP and ectopic PPARγ expression before adipogenesis (B), morphological differentiation (oil red O staining) (C; top panels, stained dishes; lower panels, representative fields under the microscope), and northern blot analysis of gene expression after adipogenesis (D).
(E–G) C/EBPα-deficient MEFs show significant defects in PPARγ-stimulated adipogenesis. Immortalized C/EBPα+/− and C/EBPα−/− MEFs infected with MSCVpuro-PPARγ2 were then infected with MSCVhygro expressing C/EBPα, followed by adipogenesis assay for 8 days in the presence or absence of Rosi. Shown are: western blot of PPARγ and C/EBPα expression before adipogenesis (E), morphological differentiation (F), and qRT-PCR analysis of gene expression after adipogenesis (G). PGAR is also known as Angiopoietin-like 4 (Angptl4). FASN, fatty acid synthase; LPL, lipoprotein lipase. All results are representative of 2–4 independent experiments. Quantitative PCR data in all figures are presented as means ± SD.
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5. Figure 4 PTIP Directly Regulates C/EBPα Expression in MEFs
(A and B) PTIP-deficient MEFs show over 5-fold decrease of PPARγ- and ligand-stimulated expression of endogenous C/EBPα. MEFs described in Figures 3A–3D were treated with 0.5 μM Rosi or DMSO for 24 hr, followed by northern blot (A) and qRT-PCR analysis (B). When RNA samples were collected, cell density was less than 80%–90%.
(C) ChIP assay of PTIP recruitment to the C/EBPα promoter in PTIPflox/flox MEFs.
(D) ChIP assay of H3K4me3 enrichment on the C/EBPα promoter in PTIPflox/flox MEFs. All results are representative of three independent experiments. Quantitative PCR data in all figures are presented as means ± SD.
Cell Metabolism  , 27-39DOI: ( /j.cmet )
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6. Figure 5
Rescue of the Adipogenesis Defect in PTIP−/− MEFs Requires Coexpression of PPARγ and C/EBPα
(A–D) PTIP−/− MEFs were established by infecting immortalized PTIPflox/flox MEFs with Ad-Cre, followed by isolation of single colonies. PTIP−/− MEFs were infected with MSCVpuro expressing PPARγ, followed by infection with MSCVhygro expressing C/EBPα or F-PTIP. Western blot analysis of ectopic PPARγ, C/EBPα, and PTIP expression before induction of adipogenesis is shown (A). Cells were cultured in the absence of Rosi. Ectopic PTIP rescues PPARγ- and ligand-stimulated expression of endogenous C/EBPα in subconfluent, undifferentiated PTIP−/− MEFs. Cells were treated with 0.5 μM Rosi or DMSO for 24 hr. C/EBPα expression was analyzed by qRT-PCR (B). Cells were induced to undergo adipogenesis for 8 days and were stained with oil red O (C). Finally, qRT-PCR analysis of gene expression after adipogenesis is shown (D). All results are representative of 2–4 independent experiments. Quantitative PCR data in all figures are presented as means ± SD.
Cell Metabolism  , 27-39DOI: ( /j.cmet )
Copyright © 2009 Elsevier Inc. Terms and Conditions

7. Figure 6
PTIP Is Required for Induction of PPARγ and C/EBPα during Adipogenesis
(A and B) PTIP is essential for induction of PPARγ and C/EBPα expression during preadipocyte differentiation. Adipogenesis of primary PTIPflox/flox brown preadipocytes was performed as in Figures 2J–2L. The basal level of PPARγ and C/EBPα expression in undifferentiated preadipocytes was determined by qRT-PCR (A). PPARγ and C/EBPα protein expression during preadipocyte differentiation was analyzed by western blot (B). Whole-cell extracts were collected every 24 hr following induction of differentiation with IBMX, DEX, and indomethacin at day 0. The 54.5 kDa PPARγ1 and 57.5 kDa PPARγ2 bands as well as the 30 kDa and 42 kDa C/EBPα isoforms are indicated by arrows. The asterisk indicates a nonspecific band.
(C) qRT-PCR analysis of C/EBPβ expression during differentiation of PTIPflox/flox brown preadipocytes. RNA samples were collected every 24 hr starting from 3 days before induction of adipogenesis (Day −3).
(D–G) ChIP assays of the recruitment of PTIP, MLL4, Pol II, and H3K4me3 signal on PPARγ1, PPARγ2, and C/EBPα promoters in differentiating (Day 4) PTIPflox/flox brown preadipocytes.
(H) Model depicting the role of PTIP in transcriptional regulation of preadipocyte differentiation (adipogenesis). PTIP regulates adipogenesis through direct modulation of PPARγ and C/EBPα expression. All results are representative of 2–4 independent experiments. Quantitative PCR data in all figures are presented as means ± SD.
Cell Metabolism  , 27-39DOI: ( /j.cmet )
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8. Figure 7
Deletion of PTIP in BAT Reduces Tissue Weight and Leads to Cold Intolerance in Mice
(A–D) PTIP deletion in BAT decreases both tissue weight and expression of brown adipocyte-enriched genes. Adipose-specific PTIP knockout mice (KO) carried the genotype of PTIPflox/−;Cre. Other littermates were used as control (Con) (see Experimental Procedures). All data were from 12- to 16-week-old mice. Representative pictures of interscapular BAT, inguinal WAT (ingWAT), and epididymal WAT (epiWAT) isolated from Con and KO mice are shown in (A). Quantitation of BAT and WAT weights in Con (n = 22) and KO (n = 8) mice is shown in (B). No significant differences in body weight, BMI, and adipose tissue weights were found among groups of control mice (Figure S4). Lack of aP2-Cre-mediated deletion of PTIP gene in WAT of KO mice was determined by quantitative PCR (n = 8) (C). Also shown: qRT-PCR analysis of gene expression in BAT of Con (n = 12) and KO (n = 8) mice (D).
(E and F) PTIP deletion in BAT leads to cold intolerance in mice. The body temperatures of mice housed in 4°C were measured every hour for 6 hr (n = 7–9) (E). qRT-PCR analysis of cold-induced gene expression in BAT of mice after cold treatment for 6 hr (n = 6) is shown (F). All values are the mean ± SEM. ∗p < 0.05; ∗∗p < 0.01. Similar results were obtained from male and female mice, but only the male mice data are shown here.
Cell Metabolism  , 27-39DOI: ( /j.cmet )
Copyright © 2009 Elsevier Inc. Terms and Conditions