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The analytical laboratory School of Biological Sciences Dr Bowater Prof. Richardson Interactions of proteins with DNA The bacterial nitrogen cycle & anaerobic.

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Presentation on theme: "The analytical laboratory School of Biological Sciences Dr Bowater Prof. Richardson Interactions of proteins with DNA The bacterial nitrogen cycle & anaerobic."— Presentation transcript:

1 The analytical laboratory School of Biological Sciences Dr Bowater Prof. Richardson Interactions of proteins with DNA The bacterial nitrogen cycle & anaerobic metabolism of bacterial pathogens Dr Brearley Dr Watmough Plant metabolic biochemistry Enzymology of Nitric oxide generation/metabolism Oxidases and peroxidases in aerobic metabolism Dr Clarke Biophysical/spectroscopic analysis of protein-protein interactions Dr Hemmings X-ray structural and mechanistic studies of enzymes Catalytic properties of metalloproteins involved in bacterial respiration which enable bacteria to exploit host cell environments in symbiosis and pathogenesis

2 The analytical laboratory School of Biological Sciences Facilities & expertise Protein expression. Protein purification Protein characterization Chromatographic analysis/purification of small molecules

3 The analytical laboratory School of Biological Sciences Protein purification Under aerobic and anaerobic conditions from Category 2 microorganisms License/authority from Defra, HSE Protein purification on analytical, pilot and larger scales

4 Protein structure determination X-ray crystallography with access to beam time on synchrotons Robotic crystallization trials Protein characterization - capabilities Structure-informed protein characterization and rationale design is available

5 Protein structure prediction and modelling Enzymology, especially of metalloproteins Variety of spectroscopic techniques Time-resolved spectroscopy of metalloproteins Fast reaction kinetics Analytical ultra centrifugation Hydrodynamic properties of proteins Protein characterization - capabilities In absence of crystal structures, protein structures are modelled

6 Isothermal calorimetry to study protein-ligand interactions True thermodynamic parameters for any reaction are obtained by ITC

7 Metabolic and small molecule analysis Gas Chromatography with liquid and headspace sampling Electron Impact GC-Mass Spectrometry Flame Ionization Detection Electron Capture Detection Fermentation products, gases, natural oils are analyzed by GC

8 Metabolic and small molecule analysis Tracer analysis Stable isotopes and radioisotopes Licensed by Env. Agency High Pressure Liquid Chromatograhy on analytical and semi-prep scale UV, DAD, suppressed ion conductivity, fluorescence & radiochemical detection HPLC-Mass Spectrometry HPLC method development is available

9 Contact details & sponsors Contacts


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