1. Tumor Necrosis Factor α Increases and α-Melanocyte-Stimulating Hormone Reduces Uveal Melanoma Invasion Through Fibronectin 
Irene Cantón, Paula C. Eves, Sheila MacNeil 
Journal of Investigative Dermatology 
Volume 121, Issue 3, Pages (September 2003)
DOI: /j x
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Effect of TNF-α on invasion of three uveal melanoma cell lines through human fibronectin. Triplicate wells of cells were treated with TNF-α (100–300 units per ml) for 20 h during the fibronectin invasion experiment. TNF-α significantly increased the invasion in all three cell lines at 200 and 300 units per ml. Results are expressed as a mean percentage of the control±SEM. |, SOM 196b (n=4 experiments);?, 177w7B7 (n=4 experiments);?, VUP (n=5 experiments). *p<0.05; **p<0.01.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Western immunoblotting for the MC-1R μg total cellular protein was loaded per gel track from three uveal melanoma cell lines (196b, VUP, and 177w7B7, as indicated at the top of each track). M is the SDS-7B molecular weight marker. A single prominent immunoreactive band was identified migrating at approximately 37 kDa (indicated) for each uveal melanoma cell type.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Effect of α-MSH on invasion of three uveal melanoma cell lines through human fibronectin. Triplicate wells of cells were incubated with α-MSH (10-11 M-10-7 M) for 20 h during the fibronectin invasion experiment. α-MSH significantly decreased uveal melanoma invasion levels in all three lines, with maximum inhibition at 10-9 M for all lines. Results are expressed as a mean percentage of the control±SEM. ¦, SOM 196b (n=5 experiments);?, 177w7B7 (n=4 experiments);?, VUP (n=6 experiments). *p<0.05; **p<0.01.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Comparison between α-MSH and MSH 11–13 KP-D-V on 196b uveal melanoma invasion through fibronectin. Both peptides significantly inhibited invasion (triplicate wells of cells were examined in all cases), but no significant differences between α-MSH (n=5 experiments) and KP-D-V (n=4 experiments) were observed at the concentrations studied. Results are expressed as a mean percentage of the control±SEM. *p<0.05; **p<0.01.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Effect of TNF-α, α-MSH, and KP-D-V on uveal melanoma invasion. TNF-α (200 units per ml) increased invasion of all three uveal melanoma cell lines, whereas α-MSH and KP-D-V (at 10-9 M) decreased invasion. The presence of α-MSH together with TNF-α significantly opposed the ability of the TNF-α to increase invasion in two out of three cell lines. KP-D-V opposed the effect of TNF-α in all three cell lines. All experiments were conducted using triplicate wells of cells. Mean values from these wells were compared to control untreated wells using Student's paired t test based on a minimum of three experiments. Values differing significantly from control values are indicated by *p<0.05, **p<0.01, ***p< Values differing significantly from TNF-α-stimulated values are indicated by #p<0.05, ##p<0.01. The total number of experiments is illustrated in each histogram and all data shown are normalized with respect to the control value for ease of presentation.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions