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Aseptic Transfer Techniques 1. Protect yourself 2. Protect the environment 3. Maintain pure cultures.

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Presentation on theme: "Aseptic Transfer Techniques 1. Protect yourself 2. Protect the environment 3. Maintain pure cultures."— Presentation transcript:

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2 Aseptic Transfer Techniques 1. Protect yourself 2. Protect the environment 3. Maintain pure cultures

3 Aseptic Transfer Techniques Aseptic – absence of organisms that may cause contamination or disease Sterile – absence of all life

4 Aseptic Transfer Techniques 1. Sterile Pipetting 2. Aseptic Transfer with Inoculating Needle and Inoculating Loop 3. Alcohol Flaming Technique

5 TSB - Trypticase Soy Broth TSA Slant - Trypticase Soy Agar Slant Turbid – cloudy (10 million bacterial cells per ml) Sterilize Inoculating Needle and Loop Always Handle to Tip - never tip to Handle Prevents dangerous aerosols


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