2 Aseptic Transfer Techniques 1. Protect yourself2. Protect the environment3. Maintain pure cultures
3 Aseptic Transfer Techniques Aseptic – absence of organisms that may cause contamination or diseaseSterile – absence of all life
4 Aseptic Transfer Techniques 1. Sterile Pipetting2. Aseptic Transfer with Inoculating Needle and Inoculating Loop3. Alcohol Flaming Technique
5 TSB - Trypticase Soy Broth TSA Slant - Trypticase Soy Agar Slant Turbid – cloudy (10 million bacterial cells per ml)Sterilize Inoculating Needle and LoopAlways Handle to Tip - never tip to HandlePrevents dangerous aerosols
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