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Paola Romagnani, Benjamin D. Humphreys  Kidney International 

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Presentation on theme: "Paola Romagnani, Benjamin D. Humphreys  Kidney International "— Presentation transcript:

1 Report on ISN Forefronts, Florence, Italy, 12–15 September 2013: stem cells and kidney regeneration 
Paola Romagnani, Benjamin D. Humphreys  Kidney International  Volume 86, Issue 1, Pages (July 2014) DOI: /ki Copyright © 2014 International Society of Nephrology Terms and Conditions

2 Figure 1 Different mechanisms and strategies for kidney regeneration. (a) Genetically labeled proximal tubule cells from SLC34a1-CreERt2; R26RtdTomato mouse (red). Lotus tetragonolobus lectin (LTA, green) defines proximal tubule. Merged signal is yellow. (b) During development, inducible enhanced green fluorescent protein-labeled parietal epithelial cells can differentiate into fully equipped podocytes forming foot process interdigitations (green) with resident Tomato-labeled podocytes (red). In addition, interdigitations of parietal podocytes are visible at the vascular pole. Hoechst staining for cell nuclei, × 63 original magnification. (c) Parietal epithelial cells that are responding to retinoic acid and differentiating into podocytes (white arrows) are evidenced by colocalization of immunofluorescence for β-gal (green) and claudin1 (red) in the kidney of RARE-lacZ transgenic mice with adriamycin nephropathy treated with retinoic acid, × 40 original magnification. (d) PAX2+LHX1+ intermediate mesoderm cells differentiated from human pluripotent stem cells. Pax2 is green and Lhx1 is red. Merged signal is yellow. (e) Formation of a self-organizing kidney in culture. Ureteric compartment is visible by phase contrast (left) surrounded by WT1+ mesenchyme (red). (f) A structure reminiscent of a glomerulus and a tubule formed in vitro from single-cell suspension of metanephric mesenchyme. × 20 original magnification. Kidney International  , 23-27DOI: ( /ki ) Copyright © 2014 International Society of Nephrology Terms and Conditions

3 Figure 2 Schematic representation of the main strategies for stem cell–based kidney regeneration. ESC, embryonic stem cells; iPSC, induced pluripotent stem cells. Kidney International  , 23-27DOI: ( /ki ) Copyright © 2014 International Society of Nephrology Terms and Conditions


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