1. Secretory phospholipase A2 is required to produce histologic changes associated with gastroduodenal reflux in a murine model 
Ashok Babu, MD, Xianzhong Meng, MD, PhD, Anirban M. Banerjee, PhD, Fabia Gamboni-Robertson, PhD, Joseph C. Cleveland, MD, Sagar Damle, MD, David A. Fullerton, MD, Michael J. Weyant, MD 
The Journal of Thoracic and Cardiovascular Surgery 
Volume 135, Issue 6, Pages (June 2008)
DOI: /j.jtcvs
Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

2. Figure 1
Schematic representation of the surgical model of creating mixed gastroduodenal (DGEA) reflux.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

3. Figure 2
Diagram demonstrating the role of secretory phospholipase A2 (sPLA2) in the arachidonic acid pathway.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

4. Figure 3
Immunofluorescence analysis of DGEA esophageal tissue in study animals demonstrating no identifiable protein in C57BL/6 mice and identifiable secretory phospholipase A2 (sPLA2) protein (red staining) in BALB/c mice (A) compared with that seen in negative control animals (B).
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

5. Figure 4
Hematoxylin and eosin analysis of mucosal thickness at 20× magnification. Significant increase in mucosal thickness in response to DGEA in BALB/c secretory phospholipase A2 (sPLA2) +/+ mice (n = 5; A) compared with C57BL/6 sPLA2−/− mice (n = 5; B) is shown.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

6. Figure 5
Ki67 immunohistochemistry demonstrating a significant increase in proliferating cells in response to DGEA in BALB/c secretory phospholipase A2 (sPLA2) +/+ mice (n = 5; A) compared with C57BL/6 sPLA2−/− mice (n = 5; B). Black arrowheads point to some of the positive nuclei as an example. HPF, High-powered field.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions