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Salt and Water Homeostasis 1. Human 2. Toad Bladder 3. Water Pore (red cell) Module C 10 June 2013 Elyse Sadow, Pouya Jamshidi, Liza Lichtenfeld Keais.

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Presentation on theme: "Salt and Water Homeostasis 1. Human 2. Toad Bladder 3. Water Pore (red cell) Module C 10 June 2013 Elyse Sadow, Pouya Jamshidi, Liza Lichtenfeld Keais."— Presentation transcript:

1 Salt and Water Homeostasis 1. Human 2. Toad Bladder 3. Water Pore (red cell) Module C 10 June 2013 Elyse Sadow, Pouya Jamshidi, Liza Lichtenfeld Keais Pope, Samantha Harrington

2 Experiment Purpose: To determine the Kidneys response to Water Load 1)9 subjects. Two x 3hr Urine collections. 2)Intervention: 5 Subjects Water-Loaded (4% TBW) 3)Control: 4 subjects, 1 cup of water 4)Measured Urine Volume, Osm, Chloride 5)Calculated Excretion of Free Water, Salt, and Urea

3 44mL/kg=~4% tbw

4 Control before vs. after

5

6 Intervention Before vs. After

7 Intervention Before vs. after

8 Excretion equations Free Water Clearance per Hour= [UVol-(UVol*UOsm/300)]/3hr UVol actual *UOsm actual =UVol 300 *UOsm 300 Salt Excretion=(2*Cl)*UVol Urea=UOsm-(2*Cl) Urea Excretion=Urea* UVol

9 Free water excretion

10 Salt excretion

11 Urea excretion

12 Experimental Set-Up

13 Time (min) % weight change ADH Experimental Data

14 Data Average Time (min) % weight change ADH

15 ADH in Action ATP cAMP AC AVP Basolateral Apical V2 receptor

16 ADH in Action ATP cAMP AC AVP Basolateral Apical AQP-2

17 Examining Water Pores in Red Blood Cells

18 Beforemixing Before mixing Aftermixing After mixing Duringmixing During mixing H2OH2OH2OH2O H2OH2OH2OH2O

19 Schematic of Stopped- flow Fluorimeter

20 Isotonic vs Hypertonic

21 Inhibition of Water Transport

22 Glycerol Flow

23 Question?


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